An intranuclear bacilliform virus associated with near extirpation of Austropotamobius pallipes Lereboullet from the Nant watershed in Ardéche, France

White-clawed crayfish, Austropotamobius pallipes, were endemic to the Nant watershed, Ardéche, France, until they were extirpated by epizootic mortality at the beginning of the twentieth century. A. pallipes were successfully reintroduced to the Nant watershed in the middle of the twentieth century. However, epizootic mortality was observed in the Nant watershed in the summer of 2000 during which time A. pallipes was extirpated from downstream regions. Dead and moribund crayfish were again detected in several episodes in summer 2001 and by October the range of A. pallipes was reduced to the headwaters of just one of the three streams in the watershed. Water quality for the watershed in summer 2001 was appropriate for crayfish habitation. Bacteriology and mycology on A. pallipes collected during several of the mortality episodes in 2001 failed to reveal a cause. However, histopathology revealed a high occurrence of intranuclear eosinophilic inclusions in hepatopancreatocytes of A. pallipes . The nuclei were hypertrophic and contained bacilliform virions consisting of a cylindrical nucleocapsid surrounded by a trilaminar envelope. Virions in section were approximately 63 × 258 nm and nucleocapsids were approximately 52 × 225 nm. It is unclear whether the intranuclear bacilliform virus was the cause of the mortality episodes or was a contributor to a disease complex involving one or several other undetected pathogens.     

Chlamydia psittaci: a suspected cause of reproductive loss in three Victorian horses

Chlamydia psittaci was detected by PCR in the lung and equine foetal membranes of two aborted equine foetuses and one weak foal from two different studs in Victoria, Australia. The abortions occurred in September 2019 in two mares sharing a paddock northeast of Melbourne. The weak foal was born in October 2019 in a similar geographical region and died soon after birth despite receiving veterinary care. The detection of C. psittaci DNA in the lung and equine foetal membranes of the aborted or weak foals and the absence of any other factors that are commonly associated with abortion or neonatal death suggest that this pathogen may be the cause of the reproductive loss. The detection of C. psittaci in these cases is consistent with the recent detection of C. psittaci in association with equine abortion in New South Wales. These cases in Victoria show that C. psittaci, and the zoonotic risk it poses, should be considered in association with equine reproductive loss in other areas of Australia.     

The effect of the grain aphid,Sitobion avenae (F.), on winter wheat in England: an analysis of the economics of control practice and forecasting systems

In 1975, 1976 and 1977 counts were made of cereal aphid populations in winter wheat fields in West Sussex and Norfolk. Data were also obtained on the area of cereals treated with insecticides to control aphids in different years, dates of application and the types of insecticide used. Most crops were treated when aphid populations were at their peak or had already started to decline. A model which simulates the damage done by Sitobion avenae (F.) to winter wheat was used to analyse the survey data and revealed a large difference between potential and achieved profits. It also showed how the value of insecticide treatment varies throughout the course of an infestation and how the value of a forecasting system depends on its timing and accuracy, the size of the aphid outbreak and its probability of occurrence.     

Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis

We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH) provides the sensitivity and spatial three-dimensional resolution to resolve metabolic signatures in processes of astrocytes and neurons deep in highly scattering brain tissue slices. This functional imaging reveals spatiotemporal partitioning of glycolytic and oxidative metabolism between astrocytes and neurons during focal neural activity that establishes a unifying hypothesis for neurometabolic coupling in which early oxidative metabolism in neurons is eventually sustained by late activation of the astrocyte-neuron lactate shuttle. Our model integrates existing views of brain energy metabolism and is in accord with known macroscopic physiological changes in vivo.     

Physiological traits and cereal germplasm for sustainable agricultural systems

Plant breeding is not a discipline that readily comes to mind when agricultural sustainability is being considered. Sustainability is normally associated with farming practices such as stubble retention, direct-drilling, or amelioration practices such as contour farming or liming, or rotation practices for nutrient management and disease control. The contribution of plant breeding will be in providing germplasm for these changed practices and devising new methods of selection. This paper reviews opportunities where plant breeding can contribute to improvements in sustainable farming practices. The emphasis is on rainfed cropping systems and cereal improvement. The main contribution for breeding is to (i) increase crop water and nutrient use so that less escapes from the root profile; and (ii) preserve the soil resource with conservation farming systems by developing cultivars specifically adapted to changed farming systems and competitive cultivars that reduce herbicide use. To achieve these outcomes identification of desirable traits, suitable selection methods and development of appropriate germplasm are discussed.     

Microinjected c-myc as a competence factor

While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggests that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells.     

Using the age shift method to determine gains from weed management for Pinus radiata in New Zealand

Understanding the effects of interspecific competition on long‐term growth of plantation forestry crops is critical to forest management decisions around methods used for weed control. A series of permanent sample plots (31) incorporating trees maintained either weedy or weed‐free until canopy closure were established across New Zealand between 2000 and 2002. We used the age shift method to characterise growth responses of Pinus radiata, in New Zealand, to interspecific competition over the first third of a typical rotation. Eight years after planting, age shifts between treatments ranged between ?0.60 years and +3.07 years. On average, the age shift due to weed control increased from 0.62 to 0.96 years between 4 and 8 years of age. This was due to divergent tree growth between weedy and weed‐free plots at 55% of the sites, while at 32% and 13% of sites, respectively, differences between treatments remained constant or decreased beyond age 4 years. The magnitude of the age shift was found to be related to the type of weed dominating early interspecific competition, with woody weeds associated with significantly greater age shifts. Continued divergence in tree growth between weedy and weed‐free treatments up to 8 years after planting at the majority of sites was an unexpected result and contrasts with previous literature. In situations where growth divergence continues beyond 2 years, managers should consider continued treatment of weeds to mitigate the detrimental impacts of on‐going competition.