Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

中国西藏小反刍兽疫的发生状况与防控

2007年7月,我国首次在西藏自治区阿里地区发生了小反刍兽疫疫病流行.从2007年7月9日到11月15日,先后在阿里地区革吉、日土、札达、改则4个县出现疫情,共20个疫点,羊发病6122只,死亡1888只.2008年6月,在那曲地区尼玛县双湖区再次发现小反刍兽疫疫情,病羊及同群羊合计6690只.为消灭该病,采取了扑杀、...     

青海藏羚羊保护浅议

通过对藏羚羊的生物学特性的陈述，分析了青海省藏羚羊的资源状况，提出了保护藏羚羊的措施和建议。     

爱与恨:农民对土地感情的两个维度

土地是农民财富的重要来源,是农民生存的重要保障,是农民精神的重要寄托,农民真诚地爱着土地.但由于土地束缚了农民的发展空间,成了农民的负担,土地的经营收入不断下降, 土地调整频繁,土地产权模糊, 农民又没有真正意义上的土地使用权,土地权利屡遭侵害,对土地的保护软弱无力,农民对土地的感情又有恨的一面.     

保润剂对烟草叶表面微结构的影响

\t\t\t\t\t目的\t\t\t\t\t考察金钱草多糖保润剂对片烟原料干燥过程的影响规律,为从动力学角度理解保润剂对片烟原料的保润效果奠定理论基础。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t测试施加金钱草多糖保润剂的许昌片烟(X2F)原料在不同干燥温度的水分含量变化,确定原料水分散失的薄层干燥动力学方程,获得内部水分有效扩散系数及干燥活化能。\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t金钱草多糖可增强片烟原料的持水能力,显著降低原料的干燥速率常数和水分有效扩散系数,添加纯水和施加保润剂原料的水分有效扩散系数分别在1.286 8×10⁻¹²~1.671 8×10⁻¹² m²/s和1.074 0×10⁻¹²~1.611 0×10⁻¹² m²/s之间,干燥活化能分别为8.42 kJ/mol和13.9 kJ/mol。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结论\t\t\t\t\t金钱草多糖可提高片烟原料的保润性能,二项分布指数函数模型和Page模型可较好地描述片烟原料水分散失历程,为从动力学角度理解保润剂对片烟干燥过程中水分散失的影响提供了思路。\t\t\t\t     

东莞市封山育林规划设计及措施的探讨

封山育林是培育森林资源,提高森林质量和覆盖度,改善生态环境的重要途径。文章分析了东莞市封山育林基础条件,提出了封山育林技术方案及实施的保障措施。     

蒙古栎秋播种育苗技术的研究

自１９９３年起对本局大趟子苗圃进行了蒙古栎秋播育苗试验,并对蒙古栎从采种、种子调制、田间管理及起苗、留床、造林等环节上形成了一套完整的科学育苗方法,避免了春播育苗种子储藏和鼠害等一系列不利问题,研究探索了最佳播种量和最佳经营密度。     

做强蔬菜产业,推进新农村建设——璧山县新堰村新农村建设的实践与思考

璧山县新堰村在推进新农村建设过程中,把蔬菜作为支撑产业千方百计做大做强,以完善基础设施夯实发展基础,以优化产业结构挖掘发展潜力,以完善服务体系提供发展支持,推动了经济社会全面发展,为纯农业村推进新农村建设找到了一条可值得借鉴的成功之路.并就建设社会主义新农村总结出3点经验,一是有一个好的产业作支撑,二是有坚强的领导作保证,三是有一套好的机制作保障.     

如何训练母鸡使用产蛋箱

产在地面垫料和棚架等产蛋箱外的种蛋统称地面蛋,地面蛋是脏蛋不能孵化,即使勉强上孵也会造成孵化器污染,增加实验室检测费用等.地面蛋过多使生产成绩大打折扣,无形中损失了很多利益.     

利用表面等离子共振技术检测牛奶中的朊蛋白

利用生物传感器——表面等离子共振传感器建立在活体动物中检测朊蛋白的方法,依据抗原—抗体特异性原理检测牛奶中朊蛋白。结果表明:应用表面等离子共振技术进行检测,1 h以内即可达到在缓冲液中标准最低检测限30 ng/mL的水平,牛奶样品的最低检测限是120 ng/mL。