Use of bovine somatotropin for milk production: the effect of time of treatment initiation on milk production of Holstein/Friesian dairy cows placed on a high-forage, low-concentrate feeding system

Sixty multiparous Holstein/Friesian cows were used in a 2 × 2 factorial design trial to determine the effect of the administration of bovine somatotropin (BST), at either 60 or 120 ± 3 d post partum, on milk production. All cows were offered ad libitum a forage mixture containing, on a dry-mailer (DM) basis, grass and maize silage in a 1:1 ratio and 4 kg d⁻¹ fresh weight of concentrate, which contained 115 and 495 g kg⁻¹ DM of neutral detergent fibre (NDF) and crude protein (CP), respectively, and 13–5 MJ kg⁻¹ DM metabolizable energy (ME). BST-treated cows received subcutaneous injections of a prolonged-release formulation of BST (sometribove). Injections were administered into the ischiorectal fossa at 14-d intervals for the remainder of the lactation. This schedule resulted in sixteen and twelve injections cow⁻¹ when treatment w as in it rate d at 60 and 120 + 3 d post partum respectively. This corresponded to treatment periods of 32 and 24 weeks, in which the production responses were measured. When compared with control cows, the administration of BST from 60 and 120 ± 3 d post partum increased mean milk yield by 1–2 and 3.3 kg day⁻¹ respectively, although only the latter milk-yield response was significant ( P <0.001). The administration of BST produced a small nonsignificant increase in DM intake, no effect on milk composition and a small but significant reduction in body condition In conclusion a significant milk-yield response can be obtained from the use of BST in high-forage, low-concentrate feeding systems if forage quality is high and treatment initiation is delayed until mid-lactation. This is illustrated by the fact that the lactation yield increased from 6054 to 6842 kg. and was obtained when using only 1.2 t of concentrate.     

西藏地区地面温度的时空分布及其异常类型研究

利用1980-2004年地面温度月平均资料,分析了近25年西藏地区地面温度空间分布的基本气候特征。同时,依据EOF(REOF)经验正交函数展开,讨论了全年及各季节地温异常空间特征和时间演变,并对地温异常类型进行了区域划分。     

Solidworks在机床夹具设计中的应用

有别与传统的CAD二维绘图,利用Solidworks绘制三维机床夹具可以非常直观、形象的显示设计结构与结果.本文介绍如何利用Solidworks软件绘制机床夹具三维图形.     

Occurrence of viral haemorrhagic septicaemia in rainbow trout Salmo gairdneri Richardson reared in sea-water

Abstract. Viral haemorrhagic septicaemia (VHS) is reported for the first time in sea-water cultured rainbow trout. Heavy mortalities with typical signs and lesions A VHS virus (serotype 1) was isolated from the diseased fish. The mortalities were caused only by the VHS virus and 80 days post transfer of trout to sea-water the mortalities reached 85%, of the initial population.
The disease was experimentally transmitted to rainbow trout, both in sea-water 3·10⁴ pfu/ml of virus or by intramuscular injection of various doses of VHS₁ (7·10¹ 7·10⁴ or 7·10⁴ pfu per fish). Death occurred in all infected groups and started earlier in sea-water. Typical signs of VHS were observed in moribund fish. Viral multiplication was demonstrated to have occurred in fish organs.     

嗜热链球菌产酸特性及其发酵乳中有机酸组分分析

选取实验室分离自自然发酵乳制品中的5株嗜热链球菌菌株为研究对象,进行单菌株发酵酸乳,在发酵期间和酸乳4℃贮藏504 h阶段,分别测定菌株发酵时间、滴定酸度、pH值、活菌数以及采用反相高效液相色谱法测定有机酸含量变化,研究嗜热链球菌发酵产酸特性及其酸乳贮藏期间后酸化及其有机酸组分的变化特性。结果表明：在42℃发酵期间,5株菌在发酵2h后pH值迅速下降,达到发酵终点时间在5～9h,发酵时间最短的是菌株IMAU10632和IMAU20772。在4℃贮藏504h期间,5株菌的pH值下降幅度在0.5左右,最终pH值稳定在4.2左右,产酸速度均较缓慢,均表现出嗜热链球菌后酸化能力弱的特点,且酸度值均在90oT以下。初步筛选出一株菌株IMAU10632,凝乳时间短、产酸速率快、后酸化能力较弱,在4℃贮藏期间活菌数维持在108CFU/mL以上。     

多年生人工草地矮生嵩草(Kobresia humilis)种群空间格局与种内关联性

植物种群空间分布格局不仅反映植物的空间分布特点,还有助于研究者了解植物利用资源的现状和生存能力。本研究以矮生嵩草(Kobresia humilis)为研究对象,按株丛斑块面积将矮生嵩草分为小斑块级株丛(0～30 cm²)、中斑块级株丛(30～80 cm²)和大斑块级株丛(>80 cm²)3个株丛级,采用点格局分析的g函数统计方法,分析了矮生嵩草各级株丛的空间格局及关联性。结果表明：矮生嵩草种群中小斑块级株丛占比高于中斑块、大斑块级株丛占比,种群中幼苗充足,正处于稳定的增长阶段;矮生嵩草小斑块、中斑块、大斑块级株丛在小尺度范围内表现为聚集分布,随着尺度的增大,所有株丛斑块的聚集程度减弱,逐渐趋向于随机分布;小斑块与中斑块、大斑块的矮生嵩草株丛在小尺度内表现为正关联。斑块聚集分布和相互共存是矮生嵩草的一种生存策略。     

Biological diversity of citrus tristeza virus (CTV) isolates in Spain

A survey of citrus tristeza virus (CTV) isolates was carried out in most citrus-growing areas in Spain. Twenty-two isolates were selected by geographical origin, cultivar of source tree, and symptoms observed on the host or in preliminary tests, and were biologically characterized.
A wide range of variation in transmissibility by aphids and symptom intensity on nine different indicator species or scion-rootstock combinations was observed among CTV isolates. Mexican lime. Citrus macrophylla , and to a lesser extent citron were the most useful hosts for characterizing these isolates, and leaf symptoms and stem pitting were the most discriminating traits. Positive correlation was observed between symptoms induced on Mexican lime and C. macrophylla , but not between the symptoms induced on these indicators under greenhouse conditions and the homologous symptoms on plants grown in the screenhouse. Some of the traits studied enabled us to establish relatively well-defined groups of isolates, but in most cases a continuous range of variation was obtained and no clear group could be defined.