花椰菜主要性状遗传规律以及性状相关性分析

总结了花椰菜的单球质量、成熟期、株高株展、花球品质等主要性状的遗传规律以及性状之间的相关性,并得出结论:花椰菜主要性状遗传规律以及性状相关性研究对育种研究具有重要指导意义,但必须注重与田间调查相结合,只有将遗传规律与田间调查有机结合才能提高育种效率,提高育种水平。     

健康家兔肠道中乳酸菌的分离鉴定

本试验选用MRS、MRS+10%家兔硬粪浸出液和MRS+10%泡菜汁3种选择培养基,对健康家兔肠道的乳酸菌群分别进行需氧和厌氧培养,根据可培养细菌菌落特征、染色特性、显微镜形态观测,共分离出7株乳酸菌株,其中需氧菌2株,厌氧菌5株。结合生化试验初步鉴定,需氧菌株分别为乳酸乳球菌和短乳杆菌,厌氧菌株分别为嗜酸乳杆菌,嗜粪乳杆菌,肠乳杆菌,乳酸乳杆菌和弯曲乳杆菌。本实验为弄清健康家兔肠道的有益菌群,以便下一步制作更易于在畜禽的肠道中定植存活的复合动物微生态制剂做准备。     

杉木叶绿体微卫星及其单倍型变异分析

杉木是我国南方重要的用材林树种,但其分子标记开发滞后于其他树种。文章利用种（属、科）间扩增法,从来源于黑松、日本柳杉等的21个叶绿体微卫星（SSR）位点中为杉木筛选适合的标记。结果表明:候选标记位点中有10个能够成功扩增,其中2个位点（CJCP1m₀04和CJCP2m₀02）具有多态性,其单倍型数量（A）分别为2和6,多样性指数（H）为0.233和0.733。多态性位点的单倍型序列分析结果表明,源于日本柳杉的CJCP2m₀02在杉木中属高变异位点,其SSR重复单元类型为（AT）n··（T）n,不同于来源物种的重复单元类型（AT）n。筛选得到的叶绿体微卫星（SSR）标记在杉木分子遗传学研究中具有重要用途。     

大学图书馆信息部门“营销导向型”组织的建立

信息服务工作是大学图书馆的核心工作,打破传统的因人设岗,建立"营销导向型"信息部门,对于提升大学图书馆的信息服务工作具有重要意义。     

新疆畜牧科学院科研项目管理信息系统的构建与实现

本文主要介绍新疆畜牧科学院科研项目管理信息系统的构建与实现,主要包括项目申请、立项、实施、检查、验收等环节的全过程管理与项目资金使用的实时管理,它的建立将有效地提升新疆畜牧科学院日常科研工作的管理效率与信息化水平。     

数字化标准色板与普通板诱杀茶园假眼小绿叶蝉比较试验研究

进行数字化标准色板与普通板诱杀茶园假眼小绿叶蝉比较试验,结果表明:数字化标准色板(以下简称黄板)比普通色板(以下简称绿板)的诱杀虫口数效果明显,试验1 d后黄板比绿板的诱杀虫口量多21.5%,试验3 d后黄板比绿板多63.0%,试验7 d后黄板比绿板多122.0%,试验14 d后黄板比绿板多134.8%,而且对茶叶没有农残。因此,建议在茶园中推广使用数字化标准色板防治假眼小绿叶蝉。     

宁夏地区荷斯坦牛成母牛淘汰情况及长寿性影响因素分析

试验通过收集出生、产犊和离群的记录,探究宁夏地区荷斯坦牛成母牛的淘汰情况,分析影响该地区奶牛长寿性的因素。对15 523头荷斯坦牛的生产寿命、在群寿命和利用胎次进行描述性统计,分析淘汰时的胎次、季节和泌乳阶段的分布情况;计算生产寿命、在群寿命和利用胎次之间的表型相关,利用固定模型分析场-出生年、出生季节、牧场规模、淘汰原因和头胎产犊月龄对长寿性的影响。结果显示,宁夏地区荷斯坦牛的平均利用胎次为2.36胎,平均生产寿命为736.59 d。荷斯坦牛成母牛在产后第1个月内的淘汰风险最高,随着泌乳阶段的延长,成母牛在每个胎次内的淘汰风险均逐渐降低;随着淘汰胎次的增加,奶牛淘汰更加集中发生在产后泌乳早期;生产寿命、在群寿命和利用胎次之间存在较高的表型相关（均>0.9）,场-出生年、出生季节、牧场规模、淘汰原因和头胎产犊月龄对长寿性有显著影响（P<0.05）,春季出生和22月龄头胎产犊的奶牛长寿性表现更好。本研究初步揭示了宁夏地区荷斯坦牛成母牛的淘汰规律和长寿性的影响因素,为宁夏地区牛群选育成母牛长寿性状奠定了基础。     

北京市规模化奶牛场运动场防雨现状及建议

运动场是保障奶牛生产力和动物福利的重要设施。2021年6—9月,频繁降雨导致北京地区多家奶牛场的运动场积水,为奶牛生产力、奶牛场生防安全、环境卫生、运动场舒适性和动物福利带来隐患。通过线上线下结合,调研了北京地区27 家规模化奶牛场的运动场防雨、排水及垫料现状。结果表明：调研奶牛场中,88.90%奶牛场配备了运动场,其中83.33%奶牛场通过设置地面倾角、设置挡雨棚、雨污分离槽及布设排水管道（沟渠）、雨后水泵排水等方式实现运动场防雨及排水。此外,针对运动场建设、运动场防雨、运动场舒适性展开讨论,以期改善运动场防雨现状,满足集约化奶牛场可持续发展需求和提升动物福利。     

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

Isolation and Identification of BPIV3 Genotype C

To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.