丹东银杏不同品种的生长比较

银杏引种丹东地区以后,经长期栽培驯化,已经能完全适应该地区的气候条件,且生长稳定。在丹东地区栽培的银杏品种有10个。调查表明,生长表现的排序依次为大梅核、小梅核、佛指、大园铃、大金坠、大马铃、大白果、龙眼、卵果佛手、洞庭皇。     

滇楸半同胞家系子代苗期性状的遗传变异

利用贵州不同气候区域13个县、市151个半同胞家系种子千粒重及1年生播种苗苗期性状高、地径进行分析研究,结果表明:滇楸种子千粒重、苗高和地径不仅在气候、地理种群、而且在各家系间均存在明显差异.种子千粒重及苗期性状具有较高的遗传力.种子千粒重和苗高遗传力分别为0.906 1和0.855,其遗传变异较丰富,遗传特性表现突出.     

Leaf stoichiometry of Leontopodium lentopodioides at high altitudes on the northeastern Qinghai-Tibetan Plateau,China

Altitude affects leaf stoichiometry by regulating temperature and precipitation, and influencing soil properties in mountain ecosystems. Leaf carbon concentration(C), leaf nitrogen concentration(N),leaf phosphorous concentration(P), and their stoichiometric ratios of Leontopodium lentopodioides(Willd.)Beauv., a widespread species in degraded grasslands, were investigated to explore its response and adaptation strategy to environmental changes along four altitude gradients(2500, 3000, 3500, and 3...     

我国营林机械的发展(四)——森林保护机械

介绍了森林保护机械设备的主要种类及其发展,分析了各种机械的特点及适用场合.     

Kan浓度对无菌苗生根影响的研究

在未加激素的MS培养基中添加不同浓度的Kan,研究其对桑树陕305无菌苗的生根抑制程度,确定转基因植株延迟筛选的适宜浓度。培养基中添加Kan6mg/L是延迟筛选的适宜浓度。     

一株基因Ⅶ型新城疫病毒的分离及分子鉴定

从一起新城疫暴发的病例中分离出1株新城疫病毒（NDV），命名为铜山分离株（TS）。运用RT-PCR技术，克隆了该分离株F基因的重要功能片段（约0.5kb)，并进行了序列测定。序列分析表明，TS分离株在F蛋白裂解位点的氨基酸顺序为^112-R-R-Q-K-R-F^117，与NDV强毒株特征相符，且具有101外的K（赖氨酸）和121处V（缬氨酸）2个特征性氨基酸，与基因Ⅶ型NDV的特性完全相符。     

Association of tumor budding with tumor-infiltrating lymphocytes and prognosis in breast cancer patients

AIM: To investigate the relationship of tumor budding with clinicopathologic parameters, tumor-infiltrating lymphocytes (TILs) of tumor microenvironment and the prognosis in breast cancer patients.METHODS: A total of 178 HE section samples were collected from the breast cancer patients treated with surgery in the First Affilated Hospital of Jinan University during Jan. 2012 to Dec. 2016. The tumor budding and stromal tumor-infiltrating lymphocytes were observed under light microscope. The correlation of tumor budding with the clinicopathologic status and TILs were analyzed by χ² test. Kaplan-Meier survival analysis and Log-rank test were used to estimate the disease-free survival (DFS) and overall survival (OS).RESULTS: High tumor budding level was associated with more positive lymph nodes, higher grade, and more lymphovascular invasion. In addition, the patients with higher tumor budding level showed fewer TILs, while the patients with lower tumor budding level had more TILs. Furthermore, the patients with higher tumor budding level had a worse disease-free survival and overall survival than those with lower tumor budding level.CONCLUSION: Tumor budding is significantly associated with adverse clinicopathological characteristics of breast cancer and negatively correlated with TILs. Therefore, tumor budding may serve as a potential biomarker to predict the prognosis of breast cancer.     

Cu2O掺杂技术研究进展

氧化亚铜(Cu2O)作为理想的低成本太阳电池材料,因其表面阻抗过大导致在实际应用中转换效率偏低.如何提高Cu2O电导性已经成为Cu2O光伏应用领域研究的关键.掺杂技术作为一种控制半导体导电类型和电阻率常见的做法,被越来越多的应用于Cu2O光伏材料制备的研究.文章综述了近年来Cu2O掺杂技术的研究进展,为高效率Cu2O基太阳电池的研究提供了参考.     

响应面法优化Xenorhabdus nematophila发酵培养基的研究

【目的】提高Xenorhabdus nematophilaYL001的抗菌活性,为该菌株杀菌活性成分的提取分离及生物农药的开发应用奠定基础。【方法】以YSG培养基为基础,采用单因子试验方法,对最佳碳源和氮源进行筛选,并采用全因子中心组合试验设计和响应面法对其最佳配比进行优化。【结果】YL001菌株的最佳碳源为玉米粉,氮源为豆饼粉,培养基的最佳组成为:玉米粉9.69 g/L,豆饼粉76.50 g/L,MgSO41.07 g/L,(NH4)2SO41.79 g/L,KH2PO40.63 g/L,K2HPO40.80 g/L,Na2SO41.25 g/L,在此条件下,YL001菌株抗菌活性达268.9 U/mL。【结论】培养基优化后YL001菌株的抗菌活性与试验的预测值接近,表明响应面法在培养基优化中十分有效,相对简单,且节省时间和材料。     

金黄色葡萄球菌α-溶血素的研究进展

目的α-溶血素在金黄色葡萄球菌(金葡菌)肺炎的感染过程中起着非常重要的作用,可作为治疗金葡菌肺炎的新靶标。因此,研究百里香酚对金葡菌α-溶血素表达的影响具有重要意义。方法测定最小抑菌质量浓度(MIC)和生长曲线并进行了溶血试验、蛋白免疫印迹分析、荧光定量PCR以及细胞毒性试验。结果百里香酚对金葡菌USA300的MIC为256 μg/mL,亚抑菌质量浓度的百里香酚(32 μg/mL)可下调金葡菌hla和RNAIII的转录水平,降低α-溶血素的表达,对溶血素介导的肺癌上皮细胞A549损伤具有保护作用。结论百里香酚具有成为新型抗金葡菌感染药物的潜力。