东亚飞蝗蝗蝻抽样的研究

自五十年代以来,应用于东亚飞蝗蝗区估计蝗蝻种群密度的抽样方法,是样方为10平方尺的筒单随机抽样。本文是以抽样数、抽样消耗、相对精确度为标准,对三种抽样模型、三个抽样单位进行了比较。根据这些结果,我们提出了在当前蝗蝻密度较低的情况下,应采用样方为100平方尺的最适配额分层抽样。这种抽样方法在精确度方面可以高出现用的抽样方法2倍以上。而抽样时间消耗可相对节约一半以上。     

棉籽壳对黄萎病菌的抑菌作用和抑菌物质的分析

根据过去观察,棉籽壳有明显抑制黄萎病菌的作用。本试验初步证明这一抑菌物质具有丹宁类物质的某些特性。不同铃龄各部位的抑菌强度总趋势,与用高锰酸钾滴定法测得的丹宁类物质含量趋势一致,即不论大、中、小铃,感病及抗病品种,种壳的抑菌作用均大于胎座。初步认为种壳中较高浓度的丹宁类物质是种子内部带菌率极低的主要原因之一。纯丹宁在棉籽饼粉酒精洋莱培养基中的抑菌作用较供试的其它培养基小,说明此培养基用于种子带菌分离的优越性。棉花枯萎病菌对丹宁的耐受力显著高于黄萎病菌。     

新春祝辞

值此农历乙酉年新春佳节之际,我谨代表中国兽医药品监察所,向工作在全国兽药科研、生产、检验、销售一线的全体同仁,以及所有关心、支持和帮助我们工作的各位领导和朋友们拜年,并致以新春的祝贺!衷心地祝愿大家在新的一年里,工作顺利,身体健康,阖家幸福,万事如意!     

The Blood-Vessel System of the Equine Periodontal Ligament: a Mesoscopic Study on Corrosion Casts

Several investigators have suggested that the blood-vessel system in the equine periodontal ligament does not only serve nutritional needs, but also specific functional needs, e.g. in terms of a shock absorbing system. In order to supplement the previous data, corrosion casts of the periodontal blood vessels were studied with special attention to the spatial arrangement and connections of the vessels. The heads of eight warm-blooded horses (age 2.5–23 years, seven female, one male), which had been killed for medical reasons, were dissected. The arteria alveolaris inferior and the a. infraorbitalis were perfused with 100 ml Heparin (20 000 IU) followed by 100 ml tap water. After storage at 4°C for 24–96 h, 10–200 ml methacrylate resin (Biodur^TM E20) were injected by use of a cartridge pistol for approx. 1 h. After polymerization for 24 h at 60°C, the jaws were frozen and cut with a steel band saw in a horizontal, sagittal or transversal plane to obtain segments that exposed the periodontal space. The specimens were macerated in detergent at 60°C until the soft tissues had vanished. Care was taken to keep the periodontal vessels complete and in topographical integrity. The vascular casts were mesoscopically examined by use of a dissection microscope (magnification 5.8×–40×). The blood vessels in the periodontal space connected with vessels in three other sites:

• (1)

  occlusal – with the blood vessels of the gingiva;

       

浅谈HACCP在机制针形名茶中的应用

对HACCP作简要介绍，根据HACCP体系的要求，分析了机制针形名茶过程中各个环节可能出现的危害因素，用HACCP的原理确立了关键控制点，并且建立了相应的控制措施。     

红星苹果和肥城桃果实软化的酶学基础

以红星苹果穴Malusdomesticacv.Starking雪和肥城桃穴Prunuspersicacv.Feicheng雪果实为试材,结合乙烯利和1-甲基环丙烯(1-MCP)处理,分别研究了2者软化的酶学基础,并就其差别作了探讨。结果表明:红星果实软化中起主要作用的酶为内切和外切多聚半乳糖醛酸酶(endo-和exo-PG);肥城桃果实软化的前20d则以纤维素酶(EGase)为主,20d后软化以endo-PG为主。研究同时发现,脂氧合酶(LOX)在这2种果实软化的启动阶段都起了明显的促进作用。1-MCP通过对红星果实中PG、肥城桃果实贮藏前期EGase和后期endo-PG活性的抑制,以及对2者软化启动阶段LOX活性的抑制,延缓了果实硬度的下降。     

新疆石河子地区沙漠增温效应对绿洲农业影响的研究

由于沙漠 ,绿洲 ,空气的比热等热性能不同 ,形成沙漠夏季温度明显高于绿洲 ,进而对绿洲地区增温 ,称沙漠增温效应。石河子地区 1 0 0 km范围内沙漠对绿洲的增温平均 1 .76℃ ( 1～ 2℃ ) ;根据绿洲距沙漠的远近与气温间存在的线性关系 ,回归方程为 :y=2 7.5 2 97- 0 .0 335 x。 r=- 0 .881 3*。沙漠增温效应对绿洲农业有多方面作用 ,形成夏季富照高温、秋季大日较差以及促成棉花在夏季的“冷害”等 ,深刻地影响绿洲特色农业、栽培技术及人们生活     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

Bone marrow-derived stromal cells and cartilage tissue engineering

Articular cartilage repair is one of the most challenging issues which remains to be resolved in clinic work. Discovering of bone marrow stromal cells (BMSC) and its application in tissue engineering provide new methods for the treatment of cartilage defects. High seeding density, appropriate cytokines and three-dimensional culture play important roles in the process of inducing BMSC differentiating into chondrocytes, suitable scaffold is also essential in reconstructing cartilage in vitro by methods of tissue engineering.