3,5-二硝基水杨酸法与酶法测定甜玉米还原糖和蔗糖含量的比较

针对目前测定甜玉米还原糖和蔗糖含量的3,5-二硝基水杨酸法和酶法进行比较研究,分析两者在育种中的应用。结果显示:酶法的测量准确度比3,5-二硝基水杨酸法高,且其稳定性和重现性都优于3,5-二硝基水杨酸法。通过两种方法对10个甜玉米杂交种种子的还原糖和蔗糖含量的测定,证实了两种方法在甜玉米还原糖和蔗糖含量的检测上是一致的。从育种实践考虑,酶法适于样品数较小的检测,3,5-二硝基水杨酸法更适合于大批量样品的检测。     

非人灵长类动物慢性肾病模型研究进展

慢性肾病(Chronic kindey disease,CKD)作为高发病率、低防治率的常见疾病之一,目前尚无非人灵长类动物在CKD模型中应用的进展报告。建立CKD模型的非人灵长类动物主要是猕猴属中恒河猴、食蟹猴和豚尾猴3种。从造模方式看,主要采用的方法有诱导性模型和自发性模型两种。诱导型造模可分为药物诱导、免疫诱导、重金属化合物诱导、辐射损伤、生物诱导等不同方法。从肾脏病理改变看,有以肾小球病理改变为主,如猪尾猴疟原虫、牛丙种球蛋白等诱导者;有以肾小管病理改变为主,如氯化镉、顺铂等诱导者;有兼顾肾小球和肾小管损伤,如猴免疫缺陷病毒(SHIV)诱导者。为利用非人灵长类动物进行CKD相关研究提供了方法学汇总,具有一定参考价值。     

立体栽培月季品种筛选试验

通过对15个月季品种立体栽培试验,筛选出最适宜立体栽培的品种8个,较适宜立体栽培的品种3个,最不适宜立体栽培的品种4个。     

施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响

【目的】明确氨基酸硒和6-BA对葡萄叶片衰老与活性氧代谢的影响,为延缓叶片衰老技术的提出提供理论依据。【方法】以设施延迟栽培条件下叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施氨基酸硒和6-BA处理,以喷施等量清水作为对照,研究施硒及6-BA对叶片衰老期间各功能叶片的叶绿素含量、净光合速率(Pn)、丙二醛(MDA)含量和超氧阴离子自由基(O2·)、过氧化氢(H2O2)含量及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)抗氧化酶活性的影响。【结果】与对照相比,外源氨基酸硒和6-BA处理显著延缓了叶片的叶绿素含量和净光合速率(Pn)的下降,提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,从而减慢了丙二醛(MDA)、超氧阴离子自由基(O2·)、过氧化氢(H2O2)的上升速率,延缓了叶片衰老。2个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。【结论】氨基酸硒和6-BA处理可有效提高叶片的抗衰老能力,延长叶片的功能期。     

阅读辅导服务在现代图书馆建设中的作用

本文主要阐明了阅读辅导的含义,论述了阅读辅导服务对现代图书馆建设的作用。     

Genetic Diversity Analysis of Cervus elaphus Using AMELY Gene in Y Chromosome

In order to determine the genetic diversity in Cervus elaphus using AMELY gene in Y chromosome,200 blood samples from Cervus elaphus yarkandensis,Cervus elaphus asiaticus,Cervus elaphus xanthopygus,Cervus elaphus songaricus and Cervus elaphus kansuensis populations were collected and AMELY genes were sequenced in this study.The haplotype diversity of Y chromosome was analyed,phylogenetic tree was built to explore the genetic diversity and the paternal origins about Cervus elaphus.The results showed that:Cervus elaphus yarkandensis had the most variation sites and the highest nucleotide diversity.The genetic distance between Cervus elaphus yarkandensis and other Cervus elaphus were far.6 haplotypes were identified in this study,named as A1,A2,A3,A4,A5 and A6,respectively.Cervu elaphus yarkandensis,Cervus elaphus asiaticus and Cervus elaphus kansuensis had separate haplotype.The NJ and ML phylogenetic trees showed that Cervus elaphus songaricus,Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus kansuensis clustered together which Cervus elaphus yarkandensis and Cervus elaphus kansuensis were form a department,separately.Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus yarkandensis clustered into one branches and there might be gene exchange among Cervus elaphus yarkandensis,Cervus elaphus kansuensis and other Cervus elaphus.     

Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms

By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.     

小白菜自交不亲和性相关基因的分子标记开发

以自交亲和系WS-199和自交不亲和系WS-85为亲本杂交获得的F_2分离群体为供试材料,采用BSA法结合SNP芯片技术,筛选出与自交不亲和性相关的SNP标记,并将SNP差异位点序列信息与白菜基因组序列进行比对分析,并进一步开发SSR标记,共获得了与自交不亲和性相关的SSR分子标记BrA1-2、BrA1-3和BrA1-14,为分子标记辅助自交不亲和性杂交种生产提供技术支持。     

广东新农村建设的现状分析与发展对策

针对广东新农村建设存在的规划滞后、资源浪费、污染严重、社会管理水平低、环保意识薄弱等问题，提出用生态文明理念引领新农村建设以及建设广东生态文明新农村的对策。     

不同营养条件对羊肚菌菌核形成的影响

菌核形成与羊肚菌(Morchella esculenta)子实体的形成有密切的关系。以PDA培养基为基础培养基,通过添加其他物质培养羊肚菌菌丝,观察菌核生长情况,从而初步判断其形成菌核的培养基限定条件。结果表明,碳源营养添加物为淀粉和乳糖,浓度为10 g·L^-1;氮源添加物为NH4HCO3、KNO3和甘氨酸,浓度为250 mg·L^-1;添加KH2PO4,浓度为1 g·L^-1,均能较好地促进羊肚菌菌核的形成。