Digital mapping of soil carbon sequestration potential with enhanced vegetation cover over New South Wales,Australia

Digital soil maps of soil organic carbon (SOC) sequestration potential resulting from a hypothetical 10% relative increase in long-term vegetation cover are presented at 100-m resolution across the state of New South Wales (NSW) in southeast Australia. This land management outcome is considered realistically achievable for many land managers, using strategies such as revegetation, grazing management or crop residue management. A mean state-wide potential increase of 5.4 Mg ha⁻¹ over the 0- to 30-cm depth interval was derived. Assuming a 20-year period of re-equilibration, this equates to an average SOC increase of 0.27 Mg ha⁻¹ year⁻¹. Sequestration potential is systematically influenced by a combination of climate, soil parent material and current vegetation cover, for example only 1.6 Mg ha⁻¹ SOC under dry conditions in sandy, infertile soil material with sparse vegetation cover, compared with 15.9 Mg ha⁻¹ under wet conditions in clay-rich, fertile soil material with moderate–high vegetation cover. The outputs could be used to identify locations of highest sequestration potential and thereby help prioritize areas and inform decisions on sequestration programmes. Future application of the method at field scale with high levels of accuracy, together with strategic sampling, may provide statistically reliable estimates of carbon sequestration, for application in carbon trading schemes such as Australia's Emissions Reduction Fund. The modelling involved a conceptually transparent ‘space-for-time substitution’ process. Multiple linear regression (MLR) and random forest (RF) modelling techniques were applied, but only MLR gave consistently meaningful results. The apparent failing of RF in this application warrants further examination.     

Spatio-temporal heterogeneity effects on seedling growth and establishment in four <Emphasis Type="Italic">Quercus</Emphasis> species

Introduction   

The great spatial and temporal heterogeneity of Mediterranean ecosystems can influence establishment success in woody species, whose natural regeneration occurs to a very small extent. In this work, the effect of the spatial pattern of environmental variables (light availability, soil moisture and herbaceous production) on seedling emergence, growth, survival and establishment success was examined by using a spatially explicit approach.     

Use of poultry manure and plant cultivation for the reclamation of burnt soils

Annual (Pisum sativum L. and Vicia sativa L.) and perennial (Trifolium repens L. and Lotus corniculatus L.) leguminous species were grown in pots containing samples from the ash layers of two Cambisols under Pinus sylvestris L., which has been affected by high-intensity wildfires 3 and 15 days before the sampling. The gramineous Lolium perenne L. was cultivated as a second plant after Trifolium and Lotus harvesting. Three treatments were compared: soils without fertilization and soils fertilized with two doses of poultry manure (1 and 2 g total N kg^-1 dry soil). The aim of the work was to study the capacity of the ash layer to sustain vegetation and the influence of plants and organic manure on the recovery of vegetation cover, ash layer fixation and soil structure formation to avoid erosion. The ash samples were able to sustain vegetation without fertilization. The organic manure increased the yields of all the plants tested, the lower dose being the optimal for the first crop whereas the higher dose was beneficial for the second crop. The annual legumes grew very quickly. The mixture of Trifolium and Lotus seemed very suitable for reclamation of soil degraded by wildfires because Trifolium produced more phytomass than Lotus in the first growing stages whereas the development of Lotus was higher in the later growing stages. Ash layer conditions did not inhibit nodulation, which was, however, stimulated by the organic manure, particularly in the case of Lotus. Lolium after perennial legumes was the best plant combination because it produced the highest phytomass, particularly root phytomass, and thus improved vegetation cover and ash layer fixation. All the plants tested improved the formation of soil aggregates, particularly the combination of perennial legumes and Lolium. However, wet aggregate stability was higher when plants were grown on soils fertilized with poultry manure than when plants were cropped on unmanured soils, which points to the favourable influence of the organic manure on soil aggregation.     

Transgenic fertile soybean plants derived from somatic embryos transformed via the combined DNA-free particle bombardment and <Emphasis Type="Italic">Agrobacterium</Emphasis> system

An Agrobacterium-mediated transformation procedure for soybean [Glycine max L. Merrill] proliferating somatic embryos is here described. The Agrobacterium tumefaciens LBA4404 strain harboring pTOK233, pCAMBIA1390-olp or pH7WG2Dwrky plasmids was used to mediate gene transfer into the plant genome. Prior to Agrobacterium inoculation, proliferative soybean embryogenic clusters were microwounded by DNA-free tungsten particle bombardment. Three independent transformation experiments were performed. In Experiment I, 26 transgenic plants were obtained from a unique clone of cv Bragg, while 580 plants were recovered from 105 clones of cv IAS5. In Experiment II, a single hygromycin-resistant clone of cv BRSMG68 Vencedora was recovered and gave rise to five plants. In Experiment III, 19 plants of cv Bragg and 48 plants of IAS5 were recovered, representing five and 14 independent transformation events, respectively. PCR and Southern analyses confirmed the transgenes’ integration into plant genomes. Transgenic plants were fertile. They flowered, set pods and seeds. Transgene segregation in two T₁ progenies fits the Mendelian pattern (3:1 transgenic:non-transgenic plants). This is the first report of transgenic fertile soybean plants obtained from somatic embryogenic tissues transformed by the system that combines DNA-free particle bombardment and Agrobacterium.     

A haptoglobin-hemoglobin receptor conveys innate immunity to Trypanosoma brucei in humans

The protozoan parasite Trypanosoma brucei is lysed by apolipoprotein L-I, a component of human high-density lipoprotein (HDL) particles that are also characterized by the presence of haptoglobin-related protein. We report that this process is mediated by a parasite glycoprotein receptor, which binds the haptoglobin-hemoglobin complex with high affinity for the uptake and incorporation of heme into intracellular hemoproteins. In mice, this receptor was required for optimal parasite growth and the resistance of parasites to the oxidative burst by host macrophages. In humans, the trypanosome receptor also recognized the complex between hemoglobin and haptoglobin-related protein, which explains its ability to capture trypanolytic HDLs. Thus, in humans the presence of haptoglobin-related protein has diverted the function of the trypanosome haptoglobin-hemoglobin receptor to elicit innate host immunity against the parasite.     

Expression of beta-nerve growth factor receptor mRNA in Sertoli cells downregulated by testosterone

Nerve growth factor (NGF) is synthesized in male germ cells. The NGF receptor (NGFR) mRNA was found in the Sertoli cells of rat testis. Hypophysectomy increased both NGFR mRNA in testis and the number of NGFR hybridizing cells in seminiferous tubules. This was suppressed by treatment with chorionic gonadotropin or testosterone, but not with follicle-stimulating hormone. The NGFR mRNA also increased after destruction of Leydig cells or blocking of the androgen receptor. This suggests that NGF produced by male germ cells regulates testicular function in an androgen-modulated fashion by mediating an interaction germ and Sertoli cells.     

Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1

The corticotropin-releasing hormone receptor 1 (CRHR1) critically controls behavioral adaptation to stress and is causally linked to emotional disorders. Using neurochemical and genetic tools, we determined that CRHR1 is expressed in forebrain glutamatergic and γ-aminobutyric acid-containing (GABAergic) neurons as well as in midbrain dopaminergic neurons. Via specific CRHR1 deletions in glutamatergic, GABAergic, dopaminergic, and serotonergic cells, we found that the lack of CRHR1 in forebrain glutamatergic circuits reduces anxiety and impairs neurotransmission in the amygdala and hippocampus. Selective deletion of CRHR1 in midbrain dopaminergic neurons increases anxiety-like behavior and reduces dopamine release in the prefrontal cortex. These results define a bidirectional model for the role of CRHR1 in anxiety and suggest that an imbalance between CRHR1-controlled anxiogenic glutamatergic and anxiolytic dopaminergic systems might lead to emotional disorders.     

Dorsal spinous process impingement syndrome ('kissing spine') in a cat: imaging appearance and surgical management

Spinal pain is an important clinical presentation in feline patients, but the underlying causes can often be difficult to elucidate. Dorsal spinous process impingement syndrome ('kissing spine' or in human patients 'Baastrup syndrome') is a significant cause of spinal pain in equine and human patients and radiographically is characterised by a close approximation of adjacent spinous processes with reactive bone sclerosis affecting these spinous processes. In this report we describe the first reported case of dorsal spinous process impingement syndrome in a cat causing spinal pain, and successful surgical management of the syndrome. The affected cat presented at 5 years of age for evaluation of a 7-month history of progressive thoracolumbar pain. Radiographs revealed close approximation of the dorsal spinous processes of the seventh, eighth and ninth thoracic vertebrae (T7, T8 and T9), with associated reactive bone sclerosis. Surgical resection of the T8 dorsal spinous process resulted in complete resolution of the clinical signs with no evidence of recurrence 9 months after surgery.