Manipulation of vertical fence wake using passive vortex generators

The effect of streamwise vortices generated from passive vortex generators was investigated to manipulate the separation bubble behind the vertical fence. The experiments were carried out in a circulating water channel and the velocity fields were measured using 2D and stereoscopic PIV method.The distance between the vortex generator and fence and the effect of the Reynolds number were investigated. In addition,the effect of boundary layer thickness was also investigated. The averaged recirculation lengths were compared with that of uncontrolled fence flow. The results showed the oscillatory variation of recirculation region appeared under the existence of vortex generators. The reduction of the separation bubble became larger when the fence was submerged in the thick boundary layer with increasing the distance between the generator and fence. When the boundary layer is thin,vortex generator can only suppress the separation bubble under the specific condition.     

不同工况柴油机排气余热回收系统试验与仿真

基于有机朗肯循环(Organic rankine cycle,ORC)设计了柴油机排气余热回收系统。建立了ORC热力学仿真模型预测系统回收性能,并对某款柴油发动机在有、无ORC作用下分别进行试验,对比了试验数据与仿真结果,验证了模型的有效性。将模型应用于不同工况下,观察不同工质流量对ORC净功率及热效率的影响,结果表明ORC净功率随着转速的增加而增加,不同工况下最大热效率均为12.1%,且对应的工质流量选择区间随着转速的提高而扩大,此区间的确定可为ORC试验时工质流量范围的选择提供参考依据。     

水貂细胞色素b基因(Cytb)克隆、序列分析及与鼬属动物的进化关系

参照水貂(Mustela vison)近缘物种线粒体基因组序列设计引物,通过克隆、测序、再拼接的方法获得6个水貂品种和左家型水貂线粒体Cytb基因全序列.用DNAstar v6.13和DnaSP4.0分析显示,水貂Cytb基因全长为1140 bp,平均碱基含量为29.8%A、13.0%G、30.3%T及26.9%C,检测到12个多态位点,全为转换.用Clusta1X1.8进行多序列比较分析表明,水貂个体间Cytb基因相似性非常高.结合GenBank检索到的14种鼬属动物同源序列,用MEGA4.0基于邻接法分别构建鼬属动物核苷酸序列和氨基酸序列系统进化树,两种进化树得出相似的拓扑结构.结果表明:我国水貂与美洲水貂亲缘较近,与欧洲水貂(M.lutreola)亲缘较远,推算美洲水貂与欧洲水貂分歧时间在中新世.     

独立学院生物技术综合实验教学改革的探索与实践

对独立学院生物技术综合实验课程的教学内容体系、教学模式、实验考核体系等方面存在的问题进行了分析,采取了相关的改革措施,并取得了一定的成效,为独立学院的实验教学提供有益参考。     

竹醋液对田间防治斜纹夜蛾和茶小绿叶蝉的增效研究

 分别在棉田和茶园开展了添加不同浓度的竹醋液对5%氟铃尿防治斜纹夜蛾1~2龄幼虫和10%吡虫啉防治茶小绿叶蝉的增效研究，结果表明：5%氟铃尿添加稀释400倍竹醋液防治斜纹夜蛾1~2龄幼虫，施药后7d增效作用显著，施用农药推荐剂量的一半可达到推荐剂量的防效。10%吡虫啉添加稀释400倍竹醋液防治茶园茶小绿叶蝉，施药后1d、3d和7d、11d增效作用均显著，施药后1d、3d施用农药推荐剂量的一半可达到推荐剂量的防效，但添加稀释600倍竹醋液，施药后1d增效作用显著，施药后3d、7d和11d增效作用不显著。     

猕猴桃全光喷雾扦插快速育苗研究

用中华猕猴桃(Actindia chinensis Planch.)‘江西79—1’、‘79—3’、‘奉新79—2’于6月中旬进行嫩枝全光喷雾扦插,苗床温度为25—30℃,叶面相对湿度为85%以上,以蛭石为扦插基质,插条基部用1000ppm吲哚丁酸进行速蘸(30秒)处理。试验结果插条发根率77.1%。插后5天产生愈伤组织,20天生根 40天移栽时单株平均根数为11.8条,平均根长6.9cm,最多根数35条,最长根17.9cm;移栽成活率85%以上。移栽苗当年新梢长2—10cm,第2年枝蔓平均长49.7cm,最长蔓137cm。     

滁州职业技术学院园林树木种类调查与分析

通过对滁州职业技术学院校园绿化树木调查整理，确定该学院校园园林树木种类约有35科、55属、74种。校园总体绿化效果较好，但植物的配置和设计还不尽合理，需进一步兼顾常绿树与落叶树、乔木与灌木等的搭配，营造多树种的合理、美观的校园。     

芝麻人工构建雄性不育基因的转化研究初报

用基因枪将人工构建的植物雄性不育基因ＴＡ２９－Ｂａｒｎａｓｅ及其标记基因ｂａｒ导入芝麻豫芝４号的离体子叶，在ＭＳ＋５ｍｇ·Ｌ－１６－ＢＡ＋１ｍｇ·Ｌ－１ＩＡＡ＋１ｍｇ·Ｌ－１ＡＢＡ＋５ｍｇ·Ｌ－１ＡｇＮＯ３的培养基上交培养１２天，转入光、暗光替培养，诱导出不定芽。经添加２ｍｇ·Ｌ－１除草剂（ＢＡＳＴＡ）的上述培养基筛选，获得了１１个ＢＡＳＴＡ抗性株系，对其中３个株系进行Ｓｏｕｔｈｅｒｎ杂交鉴定分析，证实其中２个为转基因株系，转化率为１．４６％     

Anti-3AB antibodies in the Chinese yellow cattle infected by the O/Taiwan/99 foot-and-mouth disease virus.

The O/Taiwan/99 foot-and-mouth disease virus (FMDV), a South Asian topotype of serotype O, was introduced into Taiwan in 1999. The Chinese yellow cattle infected by the virus did not develop clinical lesions under experimental and field conditions. A blocking enzyme-linked immunosorbent assay (ELISA) kit with the 3AB antigen, a polypeptide of FMDV non-structural (NS) proteins, was used to evaluate the development and duration of anti-3AB antibodies, proving active viral replication, in the Chinese yellow cattle. The specificity of the assay was 99%, as was established with negative sera from regularly vaccinated and from na?ve cattle. The sensitivity tested with sera from naturally infected animals was approximately 64% and it was lower than that obtained by serum neutralization (SN) test. Under experimental infection, the Chinese yellow cattle developed lower anti-3AB antibodies than that developed in other species. Duration of anti-3AB antibodies was traced in two herds of naturally infected animals, indicating that anti-3AB antibodies persisted for approximately 6 months after outbreaks. On the basis of this study, we propose that the Chinese yellow cattle may have natural resistance, which limits viral replication and reduces the development of anti-3AB antibodies.     

谷秆两用稻201对硒的生物富集作用研究

硒（Se）是人体必须的微量元素，采用40 mg/kg的亚硒酸钠在齐穗期开始喷施谷秆两用稻201，使大米有机硒含量达0.27 mg/kg左右（P<0.05）；同时米糠和高蛋白稻草的有机硒富集水平分别提高3到4倍（P<0.05）；为生产富硒优质米提供依据，为生产富硒食用菌提供了优质原料。