全文获取类型
收费全文 | 3444篇 |
免费 | 233篇 |
国内免费 | 414篇 |
专业分类
林业 | 339篇 |
农学 | 389篇 |
基础科学 | 262篇 |
515篇 | |
综合类 | 1285篇 |
农作物 | 233篇 |
水产渔业 | 115篇 |
畜牧兽医 | 578篇 |
园艺 | 181篇 |
植物保护 | 194篇 |
出版年
2024年 | 22篇 |
2023年 | 60篇 |
2022年 | 146篇 |
2021年 | 181篇 |
2020年 | 173篇 |
2019年 | 180篇 |
2018年 | 111篇 |
2017年 | 146篇 |
2016年 | 118篇 |
2015年 | 147篇 |
2014年 | 160篇 |
2013年 | 203篇 |
2012年 | 236篇 |
2011年 | 211篇 |
2010年 | 222篇 |
2009年 | 202篇 |
2008年 | 161篇 |
2007年 | 181篇 |
2006年 | 190篇 |
2005年 | 168篇 |
2004年 | 88篇 |
2003年 | 75篇 |
2002年 | 83篇 |
2001年 | 52篇 |
2000年 | 82篇 |
1999年 | 60篇 |
1998年 | 60篇 |
1997年 | 50篇 |
1996年 | 41篇 |
1995年 | 50篇 |
1994年 | 30篇 |
1993年 | 43篇 |
1992年 | 35篇 |
1991年 | 21篇 |
1990年 | 31篇 |
1989年 | 19篇 |
1988年 | 26篇 |
1987年 | 13篇 |
1986年 | 5篇 |
1985年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1964年 | 2篇 |
1962年 | 2篇 |
1955年 | 2篇 |
排序方式: 共有4091条查询结果,搜索用时 80 毫秒
11.
描记滑胸茧蜂属属征和褐滑胸茧蜂形态,并提供该蜂的形态图。 相似文献
12.
13.
北疆棉花不同品种叶绿素荧光特性的研究 总被引:8,自引:3,他引:5
测定了北疆6个不同棉花品种田间叶片的净光合作用、荧光参数的变化。结果表明:晴天棉花叶片的光化学效率(Fv/Fm)随着光强上升而下降,到14∶00左右降到最低值,之后又随光强的减弱逐渐回升;非光化学猝灭系数(qN)则与此相反。棉叶在晴天易发生光抑制,可能会引发反应中心的降解等破坏反应。产量较高的新陆早8号和新陆早10号的Fv/Fm、光化学猝灭系数(qP)均高,且正午过后Fv/Fm恢复较快,不仅能较强地吸收光能,同时还具有较高的PSII的活性和光能转化效率,从而将所吸收的光能有效地转化为化学能,提高光合电子传递速度,形成更多的ATP和ENADPH,为光合碳同化提供充分的能量和还原能力。 相似文献
14.
以江苏省家禽科学研究所家禽育种中心崇仁麻鸡♂×(崇仁麻鸡♂×隐性白羽鸡新品系♀)♀(配套1)和固始鸡♂×(崇仁麻鸡♂×隐性白羽鸡新品系♀)♀(配套2)为素材,进行肉用性能试验,结果表明①两配套组合体重差异极显著(P<0.01),配套2比配套1重109.6克且饲料报酬高0.171;②两配套组合龙骨长、冠长、冠高、胸宽差异不显著(P>0.05),体斜长、胫长、胫围、胸角差异极显著(P<0.01),配套2均高于配套1.③两配套组合屠体率、半净膛率、全净膛率、胸肌率、腹脂率差异不显著(P>0.05),配套1腿肌率显著高于配套2(P<0.05).④两配套组合胸肌失水率、PH值、肉色、剪切力差异不显著(P>0.05).总体而言,配套2的肉用性能优于配套1,隐性白羽鸡新品系在优质鸡配套系中应用是可行的. 相似文献
15.
AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level. 相似文献
16.
17.
哈茨木霉几丁质酶诱导及其对水稻纹枯病菌的拮抗作用 总被引:17,自引:0,他引:17
在实验室条件下分别以几丁质和水稻纹枯病菌(Rhizoctonia solani)细胞壁作唯一碳源诱导哈茨木霉(Trichoderma harzianum)菌株NF9、TC3和P1产生几丁质酶,用硫酸铵沉淀法制备几丁质酶粗提液。上述木霉菌株内切几丁质酶活性(对胶体几丁质浑浊度的减少率)分别为79.8%、74.4%和76.0%,均显著高于非诱导的阳性对照。培养第5 d几丁质诱导的木霉菌株NF9和TC3内切几丁质酶活性显著高于由水稻纹枯病菌细胞壁诱导的酶活性。体外测定表明,通过诱导的木霉菌株TC3、NF9和P1几丁质酶粗提液对水稻纹枯病病菌的拮抗圈直径可达38、21和23 mm,与非诱导的阳性对照比较有显著性差异。对木霉几丁质酶拮抗作用的特点及生防应用进行了讨论。 相似文献
18.
19.
AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus. 相似文献
20.
JIANG Xun ZENG Yao-ying HE Xian-hui XU Li-hui DI Jing-fang FENG Zheng ZHAO Jing-xian WANG Qing WANG Tong SHI Jian-bo 《园艺学报》2004,20(6):924-928
AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage. 相似文献