Over-expression of hsa-miR-150 induces re-differentiation of diffuse large B-cell lymphoma cell line OCI-Ly10

AIM:To investigate the mechanism that over-expression of hsa-miR-150 induces the re-differentiation of diffuse large B-cell lymphoma cell line OCI-Ly10. METHODS:The expression level of hsa-miR-150 in CD19+B and OCI-Ly10 cell lines was detected by real-time PCR. The expression level of c-Myb was detected by Western blotting and immunofluorescence cytochemistry methods. Lentiviral supernatant containing recombinant plasmids was transfected into OCI-Ly10 cells by LipofectamineTM 2000 and named Ly10-control and Ly10-miR-150. The biological functions of the 2 cell sublines were identified by MTT assay. The cell cycle and apoptotic rates were detected by flow cytometry. The expression levels of B-lymphocyte differentiation-related genes and c-myb in Ly10-control and Ly10-miR-150 cells were detected by real-time PCR and Western blotting. When c-myb was interfered in by interference fragment in OCI-Ly10 cells, the interference efficiency and the expression levels of BCL6 and PRDM1 were detected by real-time PCR and Western blotting. RESULTS:The expression level of hsa-miR-150 in CD19+ B cells was significantly higher than that in OCI-Ly10 cells. The expression level of c-Myb in OCI-Ly10 cells was higher than that in CD19+ B cells. The expression levels of B-lymphocyte differentiation-related genes were changed significantly in OCI-Ly10 cells after transfected with hsa-miR-150. The expression levels of PAX5, BCL6 and c-Myb in Ly10-miR-150 cells were lower than those in Ly10-control cells, but the expression levels of IRF4, PRDM1 and XBP1 were higher than those in Ly10-control cells. The expression level of BCL6 was lower and PRDM1 was higher after interference. CONCLUSION:Hsa-miR-150 plays a significant role in inhibiting proliferation and inducing apoptosis of OCI-Ly10 cells. The mechanism that over-expression of hsa-miR-150 induces OCI-Ly10 cell differentiation toward terminal B cells may be related to the down-regulation of c-myb.     

温度对牛蛙腓肠肌收缩性的影响

采用LMS-2B型二道生理纪录仪,分别记录分析浸于不同温度(20℃、25℃、30℃)任氏液中的牛蛙腓肠肌接受连续电刺激时的单收缩曲线。观察不同温度对接受连续刺激的腓肠肌收缩能力的影响。温度为20℃、25℃、30℃时对牛蛙腓肠肌最大收缩力的影响差异不显著(P>0.05),而对疲劳时间T0.5的影响差异显著(P<0.05)。温度在20～30℃之间变化时,对腓肠肌收缩性能的影响主要是影响其疲劳时间T0.5。     

一种测定原油特征温度的新方法

原油的升温过程和降温过程有着本质区别，单凭某一过程的剪切应力－温度曲线来确定析蜡点等特征温度必然存在误差。针对这一问题提出了连续升降温过程平均粘温曲线法，该方法可经在较高的温度范围内同时测定油样的析蜡点、异常点和凝点等特征温度。介绍了该方法的操作过程，利用常规方法证实了其可靠性。     

两步进样法克服高盐浓度对小分子物质分析的影响

建立一种有效克服样品中高盐浓度对毛细管区带电泳分析小分子物质影响并提高分析灵敏度的方法。方法：用两步压力增样，先进水后进样再分析，以柱效为标准优化进水及进样量。结果在盐浓度２００－６００ｍｍｏｌ／Ｌ时，优化的进水及进样量均为３７．９ｋＰａ．ｓ。     

汕优63水旱秧秧苗素质与叶蘖发展动态调查

调查结果表明：旱秧秧苗具有秧体形体小，叶蘖发生早，秧苗素质高等特点。本田叶蘖发生早而快，中后期增减趋势平稳。据此认为，在大力倡导和推广水稻旱育稀植技术的同时，应对其本田的肥水运筹作相应的改革     

新型粮食水分快速测定仪的研究

在研究粮食的阻抗－频率特性和阻抗－水分特性的基础上，设计了适用于粮食收购的新型水分快速测定仪器，它具有稳定性好，灵敏度高，响应速度快，使用寿命长等特点，已在我国粮食行业普及推广使用。     

理化因素对新疆紫草培养细胞紫草宁形成的影响

研究了在促进剂Ｃｕ２＋和抑制剂２,４－Ｄ及光的作用下,对产色素培养细胞紫草宁形成的影响。对４,１５,２５,３５和４５ｄ的紫草培养细胞的电镜观察和细胞超微结构分析的结果表明,１５ｄ加促进剂Ｃｕ２＋的新疆紫草培养细胞形成的色素颗粒（紫草宁）最多,４ｄ和加抑制剂２,４－Ｄ的培养细胞中均无明显的色素颗粒。     

耕牛四种寄生虫病的联合诊断技术应用报告

该文报告了在湖北省武穴、蕲春、浠水、团风、黄梅、黄州６ 县血吸虫病流行区,采用斑点酶联技术（Ｄｏｔ－ＥＬＩＳＡ）检测耕牛的血吸虫、捻转血矛线虫、伊氏锥虫、肝片吸虫,并与病原学检查法比较,结果阳性符合率分别为９７．８％ 、９５．５％ 、９４．３％ 和９７．３％ 。说明Ｄｏｔ－ＥＬＩＳＡ 有实际应用意义和价值,且方法快速,简便。     

锦鸡儿种子劣变过程中生理生化特性的研究

采用人工老化方法,在高温高湿(40℃、RH100%)条件下,对小叶锦鸡儿(Caragana microphlla Lam.)和柠条锦鸡儿(Caragana korshinskii Kom.)的种子进行劣变处理.同时重点测定和分析生理生化指标.结果表明:高温高湿(40℃,100%RH)人工老化法的最佳处理时间梯度为0、8、16、24、36、48 h.两种供试种子发芽率和发芽指数显著下降(P＜0.05);酸性磷酸酯酶活性显著降低(P＜0.05),与种子发芽指教呈极显著正相关,是反映其种子质量的敏感指标;可溶性糖泄漏和脂质过氧化,是种子劣变的重要原因.     

石羊河流域气候变化对参考作物蒸发蒸腾量的影响

根据甘肃省气象局石羊河流域的6个气象站近50年的观测资料，应用1998年FAO最新推荐的Penman-Monteith公式计算了50年各月参考作物蒸发蒸腾量ET₀，分析了ET₀的月际变化和年际变化特征，除武威与肃南站ET₀呈逐年显著减少趋势外，其他各站的ET₀值均表现为逐年增加趋势，各个站ET₀ 20世纪90年代较80年代均有明显增加，说明气候变化对ET₀的影响较大；并分析了平均气温、平均最高气温、年日照时数、平均风速、平均相对湿度、年降水量、年蒸发量、海拔高度与ET₀的相关性，各站ET₀与平均相对湿度相关性最好；石羊河流域ET₀空间变化也较大，从山区到绿洲平原ET₀多年平均值呈递增趋势。