Nitrous oxide evolution from structurally intact soil as influenced by tillage and soil water content

Among farmers there is a growing interest for adoption of reduced tillage practices, which has accentuated the need to understand the consequences for soil nutrient dynamics and losses. A laboratory study was conducted with structurally intact soil cores collected from two depths, 0–4 and 14–18 cm, within tillage experiments on contrasting soil types, both experiments with soil under mouldboard ploughing (MP) or shallow tillage (ST). The soil cores were adjusted to one of seven matric potentials ranging from ?1500 to ?15 hPa. The extent and regulation of nitrous oxide (N₂O) evolution as a function of tillage, depth and soil characteristics was studied by measurement of N₂O and CO₂ evolution rates, as well as nitrifying and denitrifying potentials, and subsequent data analysis by multiple linear regression models. At both sites, compaction of ST soil below the depth of tillage was significant. The vertical distribution of N₂O evolution was different in MP and ST soil, but no main effect of tillage on N₂O evolution was observed. Effects of soil variables on N₂O evolution were analysed using volumetric water content, water-filled pore space, or relative gas diffusivity (RD) to represent the effect of soil water. Using RD weakened interactions with tillage and C availability and strengthened main effects, suggesting that RD may provide a more general representation of the water effect. At 0–4 cm depth, N₂O evolution was related to NO₃^? availability in the soil with 5.1% C, but to C availability in the soil with 1.5% C. The contrasting patterns of dependencies in the different environments support the interpretation of reduced tillage and soil water content as indirect controls, via diffusional constraints, of N₂O evolution.     

Effects of interleukin-1 alpha on migration of canine neutrophils in vitro and in vivo

Inflammatory cell activation by interleukin-1 (IL-1) has been the subject of studies in laboratory animals and man, and was the aim of the present study. At physiological concentrations of 0.01-100 units/ml, human recombinant IL-1 alpha was shown to be a chemokinetic and chemotactic agent for canine neutrophils in vitro and to cause dose-dependent and selective neutrophil infiltration following intradermal injection of 1 or 250 units. The findings support a putative role for IL-1, and IL-1-induced products such as IL-8, as mediator of immunoinflammatory reactions in which neutrophil infiltration is prominent.     

A vaccine strain of pseudorabies virus with deletions in the thymidine kinase and glycoprotein X genes

A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein X (gX) and thymidine kinase, designated delta GX delta TK, was constructed and evaluated as a vaccine for protecting swine against PRV-induced mortality. Doses greater than or equal to 10(3) plaque-forming units (PFU) of this strain given to mice provided protection from challenge exposure with virulent PRV. Sera tested from mice inoculated with delta GX delta TK had high titers of neutralizing antibody to PRV, but reactivity in the same sera was not significantly different from that in sera from noninoculated mice (controls) when sera from both groups were evaluated by use of an ELISA with gX antigen produced in Escherichia coli. Compared with noninoculated pigs (controls), those given delta GX delta TK (greater than or equal to 10(2) PFU) were protected completely from lethal challenge exposure, without experiencing adverse effects on weight gain and with reduction of shedding of virulent challenge virus. Serotest results indicated that, although inoculated pigs responded with strong neutralizing antibody titers, the response of delta GX delta TK-inoculated pigs to gX, as determined by ELISA before challenge exposure, was not significantly greater than the ELISA values obtained from control pigs. The ELISA values from a group of pigs inoculated with a commercially available vaccine were significantly (P less than 0.05) higher than those of control pigs. The experimental vaccine, delta GX delta TK, was avirulent for mice, swine, and sheep, but was mildly virulent for calves (mortality, 1 of 12) and more virulent for dogs (mortality, 3 of 6) and cats (mortality, 2 of 6).(ABSTRACT TRUNCATED AT 250 WORDS)     

Multilocus Genetic Typing of Bovine Single Cells by Multiplex Polymerase Chain Reaction *

A method of simultaneous amplification at three different loci (Y-specific DNA, kappa-casein gene, growth hormone gene) in cattle is presented. The use of this procedure for genetic typing on bovine crude DNA samples from less than ten diploid cells is shown. The application of this method for embryo genotyping and animal breeding is discussed.     

Investigation of the effect of Equivac® HeV Hendra virus vaccination on Thoroughbred racing performance

Objective

To evaluate the effect of Equivac® HeV Hendra virus vaccine on Thoroughbred racing performance.

Design

Retrospective pre‐post intervention study.

Methods

Thoroughbreds with at least one start at one of six major south‐eastern Queensland race tracks between 1 July 2012 and 31 December 2016 and with starts in the 3‐month periods before and after Hendra virus vaccinations were identified. Piecewise linear mixed models compared the trends in ‘Timeform rating’ and ‘margin to winner’ before and after initial Hendra virus vaccination. Generalised linear mixed models similarly compared the odds of ‘winning’, ‘placing’ (1st–3rd) and ‘winning any prize money’. Timeform rating trends were also compared before and after the second and subsequent vaccinations.

Results

Analysis of data from 4208 race starts by 755 horses revealed no significant difference in performance in the 3 months before versus 3 months after initial Hendra vaccination for Timeform rating (P = 0.32), ‘Margin to winner’ (P = 0.45), prize money won (P = 0.25), wins (P = 0.64) or placings (P = 0.77). Further analysis for Timeform rating for 7844 race starts by 928 horses failed to identify any significant change in Timeform rating trends before versus after the second and subsequent vaccinations (P = 0.16) or any evidence of a cumulative effect for the number of vaccines received (P = 0.22).

Conclusion

No evidence of an effect of Hendra virus vaccination on racing performance was found. The findings allow owners, trainers, industry regulators and animal health authorities to make informed decisions about vaccination.     

Vesicles Cytoplasmic Injection: An Efficient Technique to Produce Porcine Transgene‐Expressing Embryos

The use of vesicles co‐incubated with plasmids showed to improve the efficiency of cytoplasmic injection of transgenes in cattle. Here, this technique was tested as a simplified alternative for transgenes delivery in porcine zygotes. To this aim, cytoplasmic injection of the plasmid alone was compared to the injection with plasmids co‐incubated with vesicles both in diploid parthenogenic and IVF zygotes. The plasmid pcx‐egfp was injected circular (CP) at 3, 30 and 300 ng/μl and linear (LP) at 30 ng/μl. The experimental groups using parthenogenetic zygotes were as follows: CP naked at 3 ng/μl (N = 105), 30 ng/μl (N = 95) and 300 ng/μl (N = 65); Sham (N = 105); control not injected (N = 223); LP naked at 30 ng/μl (N = 78); LP vesicles (N = 115) and Sham vesicles (N = 59). For IVF zygotes: LP naked (N = 44) LP vesicles (N = 94), Sham (N = 59) and control (N = 79). Cleavage, blastocyst and GFP+ rates were analysed by Fisher's test (p < 0.05). The parthenogenic CP naked group showed lower cleavage respect to control (p < 0.05). The highest concentration of plasmids to allow development to blastocyst stage was 30 ng/μl. There were no differences in DNA fragmentation between groups. The parthenogenic LP naked group resulted in high GFP rates (46%) and also allowed the production of GFP blastocysts (33%). The cytoplasmic injection with LP vesicles into parthenogenic zygotes allowed 100% GFP blastocysts. Injected IVF showed higher cleavage rates than control (p < 0.05). In IVF zygotes, only the use of vesicles produced GFP blastocysts. The use of vesicles co‐incubated with plasmids improves the transgene expression efficiency for cytoplasmic injection in porcine zygotes and constitutes a simple technique for easy delivery of plasmids.     

Predicted Concentrations for Pesticides in Drainage Dominated Catchments

Regulators need a reliable, precise and easy to use tool for predicting the occurrence of pesticides in drain water and catchments in agricultural areas. Occurrence depends on a wide range of substance and site specific factors and this study presents a simple model built on the results from simulations of a detailed model system that does not neglect or omit any of these factors. A drainage dominated sub-catchment (0.03 km²) of the Lillebaek catchment (4.4 km²) on Funen, Denmark, represented by the catchment model MIKE SHE is considered. Detailed analyses have been made with respect to geological and hydrodynamic conditions as well as measurements of pesticide concentrations in ground and surface waters. Maximum concentrations in drain water, the time for reaching this concentration and the time interval for exceeding the limit value have been derived empirically from MIKE SHE simulations using degradation rates and sorption coefficients values for 37 pesticides included in the Danish PATE database. The relatively hydrophilic bentazon and hydrophobic pendimethalin are used as model pesticides for illustration. A simple tool applicable for a wide range of pesticides has thus been designed based on detailed analyses of a limited number of pesticides. The user requirements are degradation rates, sorption coefficients, application rates and regulatory limit values for the pesticides of interest.     

Algorithms for sensor-based redistribution of nitrogen fertilizer in winter wheat

Several methods were developed for the redistribution of nitrogen (N) fertilizer within fields with winter wheat (Triticum aestivum L.) based on plant and soil sensors, and topographical information. The methods were based on data from nine field experiments in nine different fields for a 3-year period. Each field was divided into 80 or more subplots fertilized with 60, 120, 180 or 240 kg N ha⁻¹. The relationships between plot yield, N application rate, sensor measurements and the interaction between N application and sensor measurements were investigated. Based on the established relations, several sensor-based methods for within-field redistribution of N were developed. It was shown that plant sensors predicted yield at harvest better than soil sensors and topographical indices. The methods based on plant sensors showed that N fertilizer should be moved from areas with low and high sensor measurements to areas with medium values. The theoretical increase in yield and N uptake, and the reduced variation in grain protein content resulting from the application of the above methods were estimated. However, the estimated increases in crop yield, N-uptake and reduced variation in grain protein content were small.     

Risk factors associated with on-farm mortality in Swedish dairy cows

Dairy cow mortality (unassisted death and euthanasia) has increased, worldwide and in Sweden. On-farm mortality indicates suboptimal herd health or welfare and causes financial loss for the dairy producer. The objective of this study was to identify cow-level risk factors associated with on-farm cow mortality. Cows with at least one calving between 1 July 2008 and 30 June 2009 from herds enrolled in the Swedish official milk recording scheme with >40 cow-years were included. Each cow was followed from the day of calving until she calved again or left the herd (died, slaughtered or sold). The effects of potential risk factors on on-farm cow mortality were analysed using a Weibull proportional hazard model with a gamma distributed frailty effect common to cows within herd. The event of interest (failure) was euthanasia or unassisted death. An observation was right censored if the cow was slaughtered, sold, calved again or had an on-going lactation at 500 days after calving. The lactations were split into seasons (January to April, May to August and September to December) and at 30 and 100 days in milk in order to evaluate seasonal effects and the effect of disease in different lactation stages. Primiparous and multiparous cows were analysed separately. The highest hazards for both primiparous and multiparous cows were found for traumatic events and diseases, both in the lactation stage in which the cow died and in the preceding stage. The hazard was higher in early lactation and lower in 2nd parity compared to higher parities. Increased age at first calving (for primiparous cows), calving between January and April, dystocia and stillbirth also increased the mortality hazard. Differences were also found between breeds, between milk production parameters at first test milking and between management types. The results from this study show the importance of good management and preventive health actions, especially around calving, to avoid mortality in dairy cows.