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排序方式: 共有521条查询结果,搜索用时 31 毫秒
71.
Yamanaka T Bannai H Nemoto M Tsujimura K Kondo T Muranaka M Hobo S Minamijima YH Yamada M Matsumura T 《Veterinary journal (London, England : 1997)》2012,193(2):358-362
Equine influenza A virus (EIV) of the H3N8 subtype is an important pathogen causing acute respiratory disease in horses. Peramivir is a selective inhibitor of the influenza virus neuraminidase (NA). The characteristics of peramivir are not only its capacity for parenteral administration, but also its strong affinity for NA and slow off-rate from the NA-peramivir complex, suggesting that it could lead to a prolonged inhibitory effect and thus allow a lower dosing frequency. The aims of this study were to evaluate the inhibitory efficacy of peramivir against the NA activities of EIV in vitro and the treatment efficacy of a single intravenous dose of peramivir in horses experimentally infected with EIV. Peramivir inhibited the activities of NA from the seven contemporary EIV strains in vitro, with 50% inhibitory concentrations ranging from 0.10 to 0.20nmol/L. Horses treated with a single IV dose of peramivir (3000mg/600mL/animal, 7.8-9.3mg/kg of bodyweight) showed significantly milder clinical signs (pyrexia, nasal discharge and cough) with a shorter duration than control horses injected with normal saline. Moreover, the mean duration of virus shedding for the horses treated with peramivir was significantly shorter than for the control horses. These findings suggested that a single IV administration of peramivir had good potential for the treatment of equine influenza, and may help to limit the spread of the disease in the horse population. 相似文献
72.
Shinji Hiyama Toshie Sugiyama Seiji Kusuhara Takashi Uchida 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(3):223-227
The temporal expression of estrogen receptor (ER)-α and ER-β mRNA was examined in male Japanese quails. Femurs of quails receiving 17β-estradiol underwent RTPCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-α was already observed on day 0 and increased slightly during bone formation whereas ER-β was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7~15). ER-α expression simultaneously decreased slightly and ER-β levels remained very low. These results suggest that estrogen activity mediated by ER-α not only affects medullary bone formation but also bone resorption. 相似文献
73.
Selection logging is a principal management scheme in natural teak-bearing forests in Myanmar. Monitoring the spatial extent
and intensity of selection logging is important for sustainable forest management. This study applied the normalized difference
vegetation index (NDVI) image differencing method using two SPOT-5 pan-sharpened images (2.5 m spatial resolution) taken in
October 2007 and January 2009 to analyze canopy changes associated with damage from forest harvesting. According to the pixel-based
analysis, NDVI changes were larger in most logging road/log landing points whereas smaller NDVI changes were seen in most
unlogged points. NDVI changes in teak stump areas were related to distance from a logging road and the number of stumps within
the estimated crown area (a circular area with a 10 m radius from the center of each stump). A Fisher’s exact test showed that one of the main factors
causing the high NDVI change in teak stump areas was the effect of road construction. The distribution pattern of teak stumps
indicated that teak stumps with estimated crown areas that contained more than one stump had high mean change in NDVI. The spectral difference between before and after logging
revealed that logging roads had a greater effect on canopy changes than teak stumps. 相似文献
74.
Takeo Sakaigaichi Yoshifumi Terajima Makoto Matsuoka Shin Irei Seiji Fukuhara Takayuki Mitsunaga 《Plant Production Science》2013,16(2):327-332
ABSTRACT Sugarcane smut, caused by Sporisorium scitamineum, is one of the most important sugarcane diseases in Japan. Wild sugarcane, Saccharum spontaneum, is known to be a key breeding material to obtain high-yielding clones. In this study, we sought to identify Japanese wild sugarcane accessions with high resistance to smut. Thirty wild sugarcanes and three sugarcane cultivars were tested by the pinprick method. The results of the inoculation tests aided in identifying wild sugarcanes with high resistance to smut disease, namely JW90, Iriomote8, and Iriomote15. After screening the germplasm, progeny distribution of smut resistance from the inoculation test and dry matter productivity in the smut disease-free field were compared. The highly resistant wild sugarcane accession had a much better impact on progeny distribution of smut resistance compared with the susceptible accession. No relationship was found between smut resistance and dry matter productivity in both populations. 相似文献
75.
Matsuura R Moriyama H Takeda N Yamamoto K Morita Y Shimamura T Ukeda H 《Journal of agricultural and food chemistry》2008,56(2):544-549
Sakura-cha (salted cherry blossom tea) is a Japanese tea that is traditionally served at celebrations such as wedding ceremonies. The production of Sakura-cha includes the immersion of cherry blossom flowers in Japanese plum vinegar, and through this process, the byproduct (plum vinegar extract of cherry blossom) is obtained. In this study, the antioxidant activity of the plum vinegar extract of cherry blossom was examined. The plum vinegar extract of cherry blossom had a greater superoxide anion scavenging activity compared with red wine, which is a well-known strong antioxidant. Liquid chromatography-mass spectrometry analysis showed that cyanidin-3-glucoside, cyanidin-3-rutinoside, and caffeic acid were the major components in the phenolic extract prepared from plum vinegar extract of cherry blossom, and they possessed superoxide anion scavenging activity. Caffeic acid is mainly responsible for the scavenging activity of phenolic extract; the contributions of cyanidin-3-glucoside and cyanidin-3-rutinoside were minor. 相似文献
76.
Iimure T Takoi K Kaneko T Kihara M Hayashi K Ito K Sato K Takeda K 《Journal of agricultural and food chemistry》2008,56(18):8664-8671
Foam stability is an important quality trait of beer. Our previous results of two-dimensional gel electrophoresis (2DE) analyses of beer proteins implied a relationship between barley dimeric alpha-amylase inhibitor-1 (BDAI-1) and beer foam stability as judged by the NIBEM-T analyzer. To develop a novel prediction method of beer foam stability under different conditions of barley cultivar and malt modification, multiple linear regression analysis was applied. The spot intensities of major beer proteins on 2DE gel were quantified and used as explanatory variables. The foam stabilities of 25 beer samples each brewed from malt with different malt modification in one of the three cultivars (cultivars A, B, and C) were explained by the spot intensities of BDAI-1 at the 5% significance level ( r = 0.421). Furthermore, two other major protein spots (b0 and b5) were observed on the 2DE gels of Japanese commercial beer samples with different foam stability. Then, multiple regression for foam stability was calculated using these three spot intensities as explanatory variables. As a result, 72.1% of the beer foam stability in 25 beer samples was explained by a novel multiple regression equation calculated using spot b0 and BDAI-1 as positive explanatory variables and spot b5 as a negative variable. To verify the validity of the multiple regression equation and the explanatory variables, the beer foam stability in practical beer samples was analyzed. As a result, 81.5% of the beer foam stability in 10 Japanese commercial beer samples was also explained by using spot b0 and BDAI-1 as positive explanatory variables and spot b5 as a negative variable. Mass spectrometry analyses followed by database searches revealed that protein spots b0 and b5 were identified as protein Z originated from barley and thioredoxin originated from yeast, respectively. These results confirm that BDAI-1 and protein Z are foam-positive factors and identify yeast thioredoxin as a possible novel foam-negative factor. 相似文献
77.
78.
Kenichiro Sakaguchi Excel Rio S. Maylem Ramesh C. Tilwani Yojiro Yanagawa Seiji Katagiri Edwin C. Atabay Eufrocina P. Atabay Masashi Nagano 《Animal Science Journal》2019,90(5):690-695
In this study, we examined the effects of superstimulation using follicle‐stimulating hormone (FSH) followed by gonadotropin‐releasing hormone (GnRH) on buffalo embryo production by ultrasound‐guided ovum pick‐up (OPU) and in vitro fertilization (IVF). Nine Murrah buffaloes were subjected to OPU‐IVF without superstimulation (control). The morphologies of the oocytes collected were evaluated, and oocytes were then submitted to in vitro maturation (IVM). Two days after OPU, same nine buffaloes were treated with twice‐daily injections of FSH for 3 days for superstimulation followed by a GnRH injection. Oocytes were collected by OPU 23–24 hr after the GnRH injection and submitted to IVM (the superstimulated group). The total number of follicles, number of follicles with a diameter > 8 mm, and number of oocytes surrounded by multi‐layered cumulus cells were higher in the superstimulated group than in the control group (p ≤ 0.05). After IVF, the percentages of cleavage and development to blastocysts were higher in the superstimulated group than in the control group (p < 0.05). In conclusion, superstimulation improved the quality of oocytes and the embryo productivity of OPU‐IVF in river buffaloes. 相似文献
79.
To assess the effect of human interferon-alpha (IFNα) on shipping fever of Thoroughbred
racehorses subjected to long-distance transportation, an IFNα preparation was orally
administered to 48 horses three times (once daily, 3 successive days) before
transportation (IFNα group). In the control group (25 horses), maltose was administered in
the same way. These treatments induced no abnormal findings in Thoroughbred racehorses
before transportation. Immediately after transportation, significant increases in rectal
temperature were observed in both treatment groups, whereas the rectal temperature of the
IFNα group tended to be lower than that of the control group. Although WBC, Fbg, and SAA
immediately after transportation were significantly increased due to transportation in
both groups, the extent of the increases in the IFNα group was significantly smaller than
in the control group. Long-distance transportation had a relatively profound impact on
Thoroughbred racehorses, which was mitigated by IFNα treatment. 相似文献
80.
Cytology of tracheal aspirates, tracheal endoscopic and blood tests were carried out to
86 Thoroughbred racehorses presenting coughs or poor performance which were suspected to
have lower respiratory tract disease (LRTD) to assess the conditions of the disorders.
Racehorses were classified into coughing (66 horses) and non-coughing (20 horses) groups
based on clinical symptoms. Nine Thoroughbred racehorses without respiratory abnormality
were used as controls. Assessment of grades of airway mucus, cytology of tracheal
aspirates and serum amyloid A (SAA), fibrinogen (Fbg) and pulmonary surfactant protein D
(SP-D) measurements were performed. Relationships between age, gender and racing careers
were also investigated to understand the characteristics of LRTD in racehorses. Mean age
was significantly higher in non-coughing group compared to coughing group. Existence of
racing career and number of starts were significantly greater in non-coughing group
compared to coughing group. On the other hand, grades of airway mucus were significantly
higher in coughing group compared to control group. Percentages of neutrophils in tracheal
aspirates were significantly higher in coughing group compared to non-coughing and control
groups. SAA, Fbg and SP-D were higher in coughing group compared to non-coughing and
control groups indicating that condition of coughing group is in the acute phase. Positive
rate of inflammatory airway disease was significantly higher in coughing and non-coughing
groups compared to control group. It was concluded that carrying out comprehensive
evaluation including investigation on SAA, Fbg and SP-D analysis with airway assessment to
Thoroughbred racehorses which were suspected to have LRTD are useful procedure to
understand the pathological condition which aid to initiate appropriate treatment,
prognosis judgment or to advise trainers to consider altering training regimen. 相似文献