一串红温室撒播栽培技术研究

在大通植物园苟圃进行一串红温室撒播栽培试验,结果表明,撒播一串红的栽培方式,比容器培育的一串红粗壮高大,而且操作技术简单易行.     

狂犬病毒RT-PCR检测方法的建立

根据狂犬病毒保守序列设计引物,利用反转录聚合酶链式反应(RT-PCR)建立了一种检测狂犬病毒的方法,以期实现对严重危及人和动物生命安全的狂犬病毒直接检测。     

发挥资源优势，开发特色产业—阳城县演礼乡仁用杏资源开发的调查与思考

根据自然条件、资源禀赋，开发适应市场需要的绿色优质产品，可以将资源优势转化为经济优势，为农业和农村经济发展开拓新的空间．为农民收入增长开辟新的来源。阳城县演礼乡开发种植仁用杏的探索。不失为晋城市新农村建设和农村产业结构调整的一个亮点。通过对演礼乡仁用杏种植、加工和销售情况的专题调研，提出了具体对策建议。     

鹅副粘病毒感染鸡试验

本室分离的鹅副粘病毒是一侏对鹅具有料强致病毒，它也能实验感染雏鸡，且雏鸡感染后发病率均为１００％，死亡率５０％以上。人工感染的鸡其症状和病理变化均与鹅自然发病的病变相类似。从感染途径看，注射接触（混养）均能感染发病，并且在感染发病及死亡时间上也与鹅感染情况相似，因此该病毒感染鹅同时又能实验感染雏鸡，而且传播之迅速，危害之严重必须引起养禽户的重视。     

TCP/IP协议脆弱性分析

TCP/IP协议是网络发展的一个基础，但是该协议确存在着致命的弱点，盲目的使用与该协议相关的服务或应用会给网络安全带来隐患，认识到TCP/IP协议的脆弱性进对我们加强网络管理和采用安全技术手段具有指导性作用。     

Antitumor effect of heat-killed lactobacilli adhered to cervical carcinoma cells

AIM: To observe the immune property of cervical carcinoma and antitumour effect of heat-killed lactobacilli adhered to HeLa cells. METHODS: Flow cytometry was used to analyze the expression of immune molecules on HeLa cells after adhered with heat-killed lactobacilli. MTT was used to analyze the cytotoxity of lymphocytes on cervical carcinoma cells. RESULTS: Adherence of heat-killed lactobacilli to HeLa cells did not affect the expression of MHC-I molecules (P>0.05), but enhanced the expression of CD80, CD86 significantly (P<0.05) in HeLa cells. Adherence of heat-killed lactobacilli to HeLa cells also enhanced the cytotoxity of NK cells. Compared to control, the cytotoxity of CTL to HeLa carcinoma cells was significantly enhanced by the adherence of heat-killed lactobacilli to HeLa cells (P<0.05). CONCLUSION: Heat-killed lactobacilli strengthen the antitumor effect on HeLa cells.     

我国市售虾酱产品品质分析与评价

为了解我国市售虾酱产品的品质现状，探究不同品种虾酱之间的品质差异，采集了32种市售虾酱，进行了感官评价和水分、盐分、蛋白质、氨基酸态氮等理化指标的检测，对品质相关数据进行了统计分析和主成分分析，并进一步采用电子鼻和气相–离子迁移谱(GC-IMS)对气味进行了分析。结果表明，市售虾酱色泽和质地差异相对较小，而气味差异较大。理化指标中，市售虾酱的氨基酸态氮含量变异系数为33.91%，离散程度最大。在主成分分析中，提取到3个主成分，累计方差贡献率达82.32%，表明能够涵盖虾酱品质的基本信息，水分、氨基酸态氮、气味是影响虾酱综合品质的关键指标。电子鼻聚类热图表明，在欧式距离4.03处可将32种虾酱聚为4类，原料是影响聚类的主要因素。对海银虾、蜢子虾和白虾3种原料的虾酱进行GC-IMS分析，共鉴定出63种挥发性成分，主要是醇类。研究结果可为虾酱产品品质评价与生产工艺改进提供参考。     

2003-2005年SCI收录中国疾病预防控制中心论文的计量分析

通过对2003-2005年SCI收录中国疾病预防控制中心发表论文情况进行统计分析，了解该中心近几年的科研论文状况和水平，并针对分析结果提出一些建议。     

Expression and Bioinformatic Analysis of ADIPOQ Gene in Hanper Sheep

The aim of this study was to investigate the expression and biological characteristics of adiponectin (ADIPOQ) gene in longissimus dorsi muscle of Hanper sheep at different months of age.The ADIPOQ gene mRNA expression was detected in longissimus dorsi muscle of Hanper sheep at 3 different growth periods (1,7 and 13 months old) by Real-time quantitative PCR.The bioinformatics method was used to analyze the nucleotide and amino acid sequences of ADIPOQ gene,protein characteristics and network interaction protein.The results showed that ADIPOQ gene was expressed in longissimus dorsi muscle of Hanper sheep,which showed an increasing trend with the growth of the months age without significant difference (P>0.05).There was higher homology of ADIPOQ gene with Capra hircus (98.60%,99.00%) and Bos taurus (98.60%,87.00%) in nucleotide and amino acid sequences.The results of physical and chemical properties analysis showed that the molecular formula of ADIPOQ protein was C₁₁₇₂H₁₇₇₆N₃₁₄O₃₄₉S₅,the molecular mass was 26.00 ku,and the theoretical isoelectric point (pI) was 5.88,which suggested that the protein was acidic.The average value of ADIPOQ hydrophilic protein (GRAVY) was －0.403,which indicated that the protein was hydrophilic and didn’t belong to transmembrane protein.In addition,the signal peptide sequence was Met¹-Ala¹⁷,and the cleavage site was between Ala¹⁷ and Arg¹⁸.ADIPOQ existed collagen structure domain and C1Q conserved structure domain at position 45－101 and 101－237,respectively.The secondary structure mainly composed of random coil (66.95%).The construction of protein interaction network indicated that ADIPOQ might interact with ADIPOR1,ADIPOR2,CDH13 and LEP.The results provided a theoretical basis for further exploring the molecular biological functions of ADIPOQ gene in the process of muscle development in Hanper sheep.