中品95-5117抗大豆花叶病毒基因源分析

中品95-5117和中品95-5383是以中品661为亲本选育的抗东北花叶病毒病3号株系(SMV3)的大豆新品系。中品95-5383抗病基因的SCAR标记已被定位于大豆F连锁群(Chr.13),与抗病基因Rsv1紧密连锁。利用大豆F连锁群的34个对SSR标记引物及与抗病基因紧密连锁的SCAR标记SCN11及Rsv1候选基因标记Rsv1-f/r,对中品95-5117系谱亲本进行检测,结合对SMV3的抗性鉴定结果进行分析,旨在明确抗SMV3基因在系谱中的传递规律,为利用分子标记辅助选择培育抗SMV3新品种提供依据。通过SSR标记分析发现,中品95-5117和中品95-5383与亲本中品661的相似性最高,而与另外一个亲本鲁豆4号关系较远。SCAR标记SCN11检测表明,只有1份材料Mangnolid(F-53)B为感病基因型。系谱的Rsv1-f/r标记分析表明,Williams82是中品95-5117中Rsv1基因的供体亲本。抗病性鉴定发现鲁豆4号高抗SMV3,但它并不携带Rsv1基因。据上述结果推测中品95-5117中不仅含有Rsv1,还具有来自鲁豆4号的抗病基因,证明该品系比其亲本中品661具有对SMV3更强的抗性。     

入世后我国农机化的环境与发展

加入WTO对于我国农业及其生产力水平重要标志的农业机械化来说，是风险与利益并存、挑战与机遇同在。因此把握好“入世”的机遇，努力创造有利于农业机械化发展的环境条件，迅速提高我国农业牛产力水平，是我国农机化发展的重要基础，也是奠定我国农业国际竞争力的重要基础．     

Analysis of Causes and Impacts of Soft Budget Constraints of Enterprises Based on Dynamic Game for Three Players

This paper researches the behavior difference among government,banks and enterprises and how it affects soft budget constraints of enterprises and non-performing loans of banks based with dynamic game.The game model suggests that government will give banks some assistance and let them refinance enterprises,especially government has strong(incentive) to sustain SOEs in China's transitional economy.Banks will do it if the assistance from government is more than the loss from refinance enterprises.In addition the value of liquidation of enterprises and the new loans affect banks' refinancing old enterprises.     

利用“永久F2”群体剖析玉米产量及其相关性状的遗传机制

利用RIL群体构建了一套包含441个杂交组合的玉米“永久F₂”群体,通过253个SSR标记的等位基因频率分析,发现“永久F₂”群体的遗传组成与基因频率与F₂群体相似,理论上可以代替F₂群体进行相关遗传研究。通过两年一点的田间试验,利用复合区间作图法对玉米产量及其3个主要构成因子进行了遗传分析。结果表明,玉米产量与穗长、穗行数及百粒重呈显著或极显著正相关,而穗长与穗行数和百粒重显著负相关。共定位3个产量QTL、8个穗长QTL,11个穗行数QTL和5个百粒重QTL。     

同轴膜片加载圆波导色散特性研究

用场论的方法分析了同轴膜片加载圆波导系统中的场,利用加载区的场匹配条件以及中心互作用区与加载区的场匹配条件,获得同轴膜片加载圆波导结构的色散方程.利用该色散方程,得到色散特性与HFSS仿真软件模拟结果良好符合.     

Hydrolysis and Condensation of Silane Agent for Metallic Surface Treatment

Novel surface treatment technique by silane film is developing for metallic anti corrosion and coat improvement. The key points, hydrolysis and condensation, for the stability of the Silane agent(SA) solution is investigated, and the effective factors of stability for silane agent solution and the relative rule are discussed. It is found that stability of SA solution is related to the temperature, pH, solvents, SA concentration and additive by studying the state of the solution and the conductivity-time curve of SA solution. Meanwhile, controlling factors and improving methods for SA stability are proposed to guide the practical application of metallic surface anti-corrosion treatment by SA.     

抗谷胱甘肽-S-转移酶(GST)单克隆抗体的研制及初步应用

以纯化的谷胱甘肽转移酶标签蛋白(GST)免疫Balb/C小鼠,取免疫鼠脾细胞与NSO骨髓瘤细胞按常规方法融合,用纯化的GST经间接ELISA筛选,阳性孔经3次有限稀释法亚克隆,最终获得2株抗GST的单克隆杂交瘤细胞株,分别命名为4B7-F4和4B7-F4.间接ELISA检测4B7-E4、4B7-F4细胞培养上清的效价分...     

吉林省鲜食玉米主推品种的耐低温性鉴定

吉林省春季播种时期土壤低温持续时间长,春播时节低温冷害是制约吉林省春播出苗的因素之一.在相同田块以及早播的条件下,不同玉米品种表现出低于适播条件下的平均出苗率,同时早播条件下平均出苗率的标准差和变异系数均高于适播条件,说明采用提前播期的方法作为筛选玉米品种种子质量的田间筛选与鉴定方法是可行的.利用此方法实验并分析数据,筛选出7份在吉林省地区早播出苗率表现较好的鲜食玉米品种,分别为京科甜126,京花糯2008,万甜1826,京科甜183,吉甜6号,京科甜2000,吉甜3号.     

冷链物流中果蔬产品包装工艺

冷链物流是新型的储运模式,由于冷藏能最大限度地保持食品的新鲜程度、色泽风味和营养成分,冷链物流成为公认最好的食品储运形式。冷链物流中的果蔬包装,必须保护产品克服外部侵蚀,如:氧化、水蒸气、微生物侵扰、灰尘污染等。为保持冷气流通,包装箱开有一些圆孔。介绍了果蔬冷链物流适用的包装工艺方法。     

Effects of apelin-13 on transient sodium currents in rat ischemic ventricular myocardial cells

AIM：To investigate the effects of apelin on ventricular arrythmias and cardiac functions in rat Langendorff perfusion-simulated myocardial ischemia model by observing the changes of transient sodium currents (INa) in normal cells and the simulated ischemic cells in rat left ventricle. METHODS：Ventricular cells were enzymatically isolated by the Langendorff perfusion system. INa was recorded by the technique of whole-cell patch-clamp. Some elements in the extracellular fluid were changed to simulate the normal or ischemic status. Forty Wistar rats were divided into 4 groups：normal group, ischemic group, normal with apelin group and ischemic with apelin group. The effect of apelin-13 on INa was observed. The method of rat Langendorff perfusion was used to simulate the ischemic heart model. The ventricular arrhythmia scores and heart functional parameters were compared. The expression level of sodium channel protein,type V,alpha subunit (SCN5A) in ventricular ischemic cells was measured by Western blotting. RESULTS： Apelin-13 increased INa amplitude in both normal myocardial cells ［(-86±13） pA/pF］ and ischemic myocardial cells ［(-52±15） pA/pF］. The results of current-voltage curve analysis indicated that apelin-13 did not change the conduction velocity of INa in the 4 groups ［(3.2±0.2） pS/pF, （3.1±0.3） pS/pF,（2.9±0.1）pS/pF and （2.8±0.4） pS/pF,respectively, P>0.05］. The membrane potentials at 50% maximal activation in the 4 groups were （-21.9±0.6） mV, （-28.7±0.3） mV, （-30.5±0.7） mV and （-36.8±0.2） mV, respectively, and the slope of activation curves was 5.6±0.3, 5.1±0.4, 4.3±0.3 and 4.9±0.6 (P>0.05), respectively. No difference of ventricular arrhythmia scores between normal group and normal with apelin group, as well as between ischemic group and ischemic with apelin group was observed. LVEDP in normal with apelin group was lower than that in normal group.The dp/dtmax and dp/dtmin in normal with apelin group were higher than those in normal group. Apelin improved cardiac function parameters in the ischemic hearts. The expression of SCN5A was not affected by apelin (28.8±3.6, 29.4±4.1, 30.1±2.9 and 31.3±3.8,respectively,P>0.05). CONCLUSION：Apelin-13 changes the gating properties of sodium channel, enhances the peak INa and facilitates the opening of sodium channel without inducing ventricular arrhythmias. Apelin-13 has a positive inotropic effect on both normal and ischemic hearts.