Development of integrated reactors for domestic garbage and town sludge

Aiming at domestic garbage aerobic compost and town sludge anaerobic treatment, on the basis of reactor TISTD (the integrated of sludge thickening and digestion)which is developed by the authors’ team, a TIDGTS (the integrated of domestic garbage and town sludge)reactor is designed.The reactor is made up of garbage and sludge chambers.Aerobic compost is adopted in the garbage chamber, while anaerobic fermentation is used in the sludge chamber, thus garbage and sludge can be treated in the same reactor.32~36 ℃ of sludge digestion can be ensured by the heat from sludge digestion, and 20 L sludge and 15 kg garbage can be treated per day.While inlet sludge moisture content is 97.2%~99.8% and VS/TS is 0.45~0.69, the discharged sludge moisture content is 92.7%~96.1% and VS/TS is 0.28~0.42.When the inlet garbage moisture content is 73.5% and VS/TS is 0.65~0.88, the discharged garbage moisture content is 48.6% and VS/TS is 0.32~0.44.The results show that the efficiency of concentration and digestion is near to TISTD which is heated by heating wire.     

Circulating current control method for inductive power transfer system

Inductive power transfer (IPT)technology allows electrical energy to be transferred between two loosely coupled inductors over relatively large air gaps based on electromagnetic induction. In current-fed IPT systems,resonant capacitor voltage distortions and current spikes on the semiconductor switches are easily caused when switches isn’t switched at zero crossing point of resonant voltage. As a result,the EMI level of the system and the switching loss are increased and thus the system transmission stability and efficiency decrease. The article analyzes the production mechanism of circulating current in load resonant high frequency inverter and points out that the resonant frequency change is the real cause. By analyzing the characters of circulating current before and after the zero-crossing points of resonant voltage and relationship between switching frequency and resonant frequency,this paper proposes a method of real-time detection for the direction of circulating current,and regulating the switching frequency in accordance with resonant frequency dynamically to ensure the switching of switch tubes at the zero-crossing points of the resonant capacitor voltage,and thus to inhibit circulating current. An experiental system with the platform of FPGA is set up to verify the effectiveness of this control method.     

福建平潭岛景观格局时空演变规律

在遥感、地理信息系统的支持下,基于2003、2011年Landsat-7卫星影像数据,运用Fragstats3.3景观格局分析软件和景观生态学的基本原理、方法,通过对比2003、2011年景观格局指数,分析研究福建省平潭岛的景观格局时空演变规律。结果表明:研究区景观各要素特征发生了较大变化,城镇、农村居民点的斑块面积由2003年的2464.08 hm2增加到2011年的4550.83 hm2;景观空间结构的变化表现为景观类型的形状指数和分维数都有不同程度的增加;邻近景观指标总体呈下降趋势,表明景观类型之间距离更远,变得更离散;景观异质性指数中的廊道斑块密度明显增大,增加量为21.96个.hm-2。8 a间平潭岛的景观格局呈现剧烈变化态势。     

不同等级普洱茶的化学成分及抗氧化活性比较

选用高、中、低三类不同等级的普洱茶为研究对象,研究比较了它们的抗氧化活性、香气成分及其主要理化成分含量的差异。结果表明,高、低两类等级的普洱茶相比,总氧化活性、清除羟自由基活性、清除DPPH自由基活性、醇类香气成分总量、杂氧化合物香气成分总量、黄酮总量以及茶色素含量等,一般都存在显著性差异(P<0.05);而中等级的普洱茶与高或低等级的普洱茶相比,除总氧化活性、清除羟自由基活性、茶多酚含量和黄酮总量存在显著性差异外(P<0.05),其他理化成分的含量一般都不存在显著性差异(P>0.05)。主成分分析表明,抑制羟自由基活性、总抗氧化活性、黄酮总量、茶褐素含量以及醇类香气成分总量等是区分普洱茶等级的重要理化指标。     

广西六堡茶“金花”菌的分离与分子鉴定

从广西银泰六堡茶厂2008年产(编号6918)的六堡茶中分离纯化得到一株特征菌株"金花"菌,用传统的形态学分离鉴定,观察菌落特征,并运用光学显微镜观察菌株形态。同时结合现代分子生物学DNA测序,在分子水平上对分离菌株进行鉴定。根据菌株的表观形态、光学显微镜和DNA序列分析,通过ITS序列同源性搜索比对及系统发育树的构建,确认该菌株为Eurotium niveoglaucum。     

茶树氧甲基转移酶基因的克隆及原核表达

获得了一类茶树氧甲基转移酶基因cDNA全长并构建了该基因的原核表达载体。以从茶树叶片中提取的总RNA为模板,结合RT-PCR与RACE克隆技术获得氧甲基转移酶(O-methyltransferase)基因cDNA全长1 280 bp,其开放阅读框为1 068 bp,编码355个氨基酸,推测的蛋白分子量为39.1 kD,理论等电点为5.68。其氨基酸序列与葡萄和蓖麻氧甲基转移酶基因相似性分别为73%、71%。将该基因片段连接到原核表达载体pET-28a中,转化大肠杆菌BL21后诱导重组蛋白的表达,经IPTG诱导,SDS-PAGE检测到1条与预测融合蛋白分子量相符的外源蛋白。     

玉米优异核心种质资源多基因矮生系5003及其姊妹系5005创制

玉米优异核心种质5003及其姊妹系5005是我国第一个自主选育的多基因控制的矮源资源。5003(5005)及其衍生自交系适应性广、抗逆性强、配合力高,与国内外主要种质类群均有明显杂种优势,构成了新的矮生杂种优势群。从20世纪80年代至今,全国各地育种单位育成含有5003(5005)种质的优良自交系25个,配出年推广面积超过10万hm2以上的玉米杂交种有38个,累计推广面积达1.08亿hm2。     

腹泻犊牛大肠杆菌血清型抗菌药物耐药性耐药基因鉴定及脉冲场凝胶电泳分型

本试验对2011年由哈尔滨周边地区采集的263份腹泻犊牛的肛拭子进行大肠杆菌的分离鉴定,并通过小鼠致病性试验鉴定出26株致病性大肠杆菌,用Kirby-Bauer法开展对12种药物的药敏试验,PCR检测了我国流行广泛的9种耐药基因,脉冲场凝胶电泳(PFGE)进行基因分型.检测结果,该地区主要流行的致病性血清型为O26、O78和O15,所有致病性菌株都存在多重耐药现象,其中阿莫西林-棒酸、链霉素、四环素、复方新诺明的耐药率达到了100％,共检出7种耐药基因,检出率符合我国平均分布趋势,且检出率基本上与耐药表型相一致.PFGE结果显示,该地区存在致病性菌株的克隆传播.     

小鼠Dazl基因融合蛋白表达载体的构建及转染

根据NCBI数据库上公布的小鼠Dazl基因的mRNA序列设计引物,以小鼠睾丸组织RNA为模板,RT-PCR扩增小鼠Dazl基因编码区片段,并将其克隆到增强型绿色荧光蛋白表达载体pEGFP-C1中,构建重组融合蛋白表达载体pEGFP C1-Dazl,单双酶切和测序验证正确.将pEGFP-Cl-Dazl质粒转染293和NIH 3T3细胞,荧光显微镜下观察到融合表达的绿色荧光蛋白,且呈胞质表达;对照组转染pEGFP-C1,绿色荧光遍布整个细胞.Dazl蛋白的免疫荧光试验也证明重组载体转染后,Dazl基因和GFP共同定位于胞质部分.pEGFP C1-Dazl融合蛋白表达载体的成功构建为进一步研究生殖特异性基因Dazl在小鼠和大型动物的表达特性奠定了一定的基础.