喷头水量分布信息传感测试机理分

针对传统喷头水量分布特性测试手段落后的问题，研究了喷头水量分布特性自动测试的关键技术，并根据水量测试原理，建立了水量信息传感器的转换模型，实现了水量信息从模拟量到数字量的一步转换。采用基于分布式总线技术的水量信息采集方案，设计了喷头水量分布特性自动测试系统，使水量分布信息和其他测试数据快速直观地显示在计算机屏幕上，并可打印输出，提高了喷头性能测试的工作效率和水平，对新型喷头的试验研究提供了可靠数据和分析手段。     

入世后我国农机化的环境与发展

加入WTO对于我国农业及其生产力水平重要标志的农业机械化来说，是风险与利益并存、挑战与机遇同在。因此把握好“入世”的机遇，努力创造有利于农业机械化发展的环境条件，迅速提高我国农业牛产力水平，是我国农机化发展的重要基础，也是奠定我国农业国际竞争力的重要基础．     

烟气脱硫泵在我国农村小火电厂应用探讨

通过对目前我国农村现有的小火电厂现状进行分析,找出其落后原因以及对环境造成污染的因素.结合目前国家相关政策,对火电厂进行烟气脱硫改造,并通过对烟气脱硫泵发展和结构的研究,指出烟气脱硫泵在农村小火电厂技术改造中占有重要作用.     

华北平原滴灌灌溉频率对萝卜生长的影响

研究了华北平原秋萝卜滴灌条件下 ,不同滴灌频率处理对萝卜叶面积系数、叶绿素含量、萝卜周径膨大、萝卜干重积累、根长密度、根干重密度等萝卜生长参数的影响并比较了各处理间萝卜产量的差异 ,发现在华北平原下半年降雨较多的情况下种植秋萝卜 ;1滴灌频率无论是 1 d1次还是 8d1次 ,对萝卜生长发育都没有明显影响 ;2滴灌条件下萝卜的平均重量和产量大大高于地面灌溉     

芥蓝二氢黄酮醇4–还原酶基因BoaDFR的克隆与功能鉴定

以白花芥蓝‘四季粗条’为材料克隆了Boa DFR基因,Gen Bank登录号为MT472647,CDS序列长为1158 bp,编码385个氨基酸,与甘蓝型油菜、甘蓝和白菜等具有高度同源性。Boa DFR表达水平在芥蓝不同发育时期、不同器官中存在显著差异,随植株发育总体呈现先下降后上升的趋势,在幼嫩种子中表达量最高。芥蓝原生质体的亚细胞定位结果显示Boa DFR定位在细胞核上。构建了农杆菌介导的芥蓝瞬时过表达体系,将Boa DFR转入白花芥蓝‘四季粗条’和黄花芥蓝‘福州黄花’中,发现Boa DFR在两种芥蓝中均高水平表达,转基因植株叶片颜色明显变紫,花青素苷显著积累,总含量最高达461.43μg·g-1 FW,而野生型和转空载体芥蓝叶片中几乎检测不到花青素苷。通过HPLC在转基因植株叶片中共检测到8种花青素苷,均为矢车菊花青素苷,其中矢车菊–3–阿魏酰–丙二酰–槐糖苷–5–葡萄糖苷占比最高。     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

3个草莓品种在辽阳地区温室栽培比较试验

为优化辽阳地区温室栽培草莓品种,笔者于2018年8月在辽阳县亚新农业合作社进行'香野'红颜'甜查理'温室栽培试验.结果表明,新品种'香野'果实甘甜爽口、香味浓郁、口感好、可溶性固形物含量高、抗病性强,是一个早熟、高产、优质的草莓新品种,可作为'甜查理'的替代品种在辽宁辽阳地区种植.     

奶牛养殖粪水还田对苜蓿产量及土壤理化性质的影响

为明确规模化奶牛养殖场粪水发酵处理后还田对苜蓿生长和土壤的影响,试验设置了追肥(苜蓿专用配方肥N∶P₂O₅∶K₂O为4∶13∶40)225 kg/hm²、追肥112.5 kg/hm²+粪水360 m³/hm²和只追施粪水360 m³/hm²3个处理。结果表明,追肥112.5 kg/hm²+粪水360 m³/hm²处理下,2019、2020年全年苜蓿干草产量分别达到15 506.5和15 548.0 kg/hm²,显著高于其他处理。追肥112.5 kg/hm²+粪水360 m³/hm²和只追施粪水360 m³/hm²两个处理显著提高了土壤有机质含量和土壤pH,而含盐量则降低。追施粪水360 m     

Soil bacterial characteristics between surface and subsurface soils along a precipitation gradient in the Alxa Desert,China

Bacteria in desert soil have unique phylogeny and important ecological functions, and theirresponses to changes in precipitation need further attention. However, relevant studies have mainlyfocused on the surface soil, and studies on the responses of bacteria at different soil depths to variationsin precipitation are rare. Thus, we used 16S rDNA high-throughput sequencing to investigate the changesin soil bacterial distribution along a mean annual precipitation gradient (50–150 mm) in the Alxa Desert,China, and compared the variation characteristics in the surface soil layer (0–10 cm) and subsurface soillayer (10–20 cm). Results showed that soil bacterial communities significantly changed along theprecipitation gradient in both soil layers. However, the subsurface soil layer could support bacterialcommunities with higher diversity and closer internal relationships but more internal competition than thesurface soil layer. Additionally, compared with the surface soil layer, variations in diversity andco-occurrence patterns in the subsurface soil layer were more in line with the changes in the mean annualprecipitation, while bacterial community structure was less variable in the subsurface soil layer. Comparedwith the mean annual precipitation, soil moisture had little influence on the structure and diversity of soilbacterial community but had a high correlation with intercommunity connectivity. Therefore, soilmoisture might play a complex role in mediating environmental conditions and soil bacterial communitycharacteristics. Due to the different responses of surface and subsurface soil bacteria to the changes inprecipitation, it is necessary to distinguish different soil layers when predicting the trends in desert soilbacterial conditions associated with precipitation, and prediction of subsurface soil bacteria may be moreaccurate.     

辽宁省2005年审定水稻品种米质情况分析及改良对策

以2005年辽宁省审定的32个水稻品种为材料,对辽宁省水稻品种品质近况进行了分析。在所有的材料中,6项指标全部达到国标一级标准的有3个,达标率为9.38%;达标率最高的是糙米率,其次是胶稠度、整精米率,最低的是直链淀粉含量。同时对辽宁省水稻品质改良中需要着重解决的几个问题进行了探讨。