Entamoeba histolytica causes intestinal secretion: role of serotonin

Lysates of the protozoan parasite Entamoeba histolytica altered active electrolyte transport when present on the serosal surface of rabbit ileum and rat colon. The lysate-induced effects on electrolyte transport were similar to those caused by serotonin, and were blocked by bufotenine, an analog known to inhibit the action of serotonin. The transport effects were partially inhibited by antibody to serotonin. The amebic lysates were shown to contain serotonin by radioimmunoassay, high-performance liquid chromatography, and thin-layer chromatography. These results suggest that the serotonin present in Entamoeba histolytica may be important in the diarrhea seen in amebiasis.     

Nitrogen fertilizer application rates on cereal crops according to available mineral and organic soil nitrogen

From all plant nutrients N fertilizer rates deserve highest attention as too high rates may result in nitrate leaching, volatilisation of N₂O (greenhouse gas) and affect the farmers’ profit. Too low rates will also depress the profit. The problem is accentuated by the fact that crops not only feed from soil inorganic but also from organic soil N. Most soil N tests do not consider the available organic soil N. The Electro-Ultra-Filtration (EUF) method applied by us takes into account the EUF extractable inorganic and organic soil N for calculating the N fertilizer rate. This method developed at the Liebig University Giessen is called Giessen model (sampling in autumn out of the upper soil layer, 0–30 cm). We compared it with the standard soil N test the “Nmin method” recommended by German officials which method does not consider the available organic soil N (sampling in spring out of three or two soil layers, depending on soil depth). The investigation was carried out on farmers’ fields on five different sites with winter cereals (wheat, barley) in 1989/1990, 1990/1991 and 1991/1992. Recommended fertilizer application rates differed somewhat for both methods. Of the 23 cases, significantly higher grain yields were obtained five times by Nmin and four times by EUF; otherwise grain yields did not differ significantly between both methods. Grain yield and crude protein concentration were increased by fertilizer N compared with the plots without N fertilizer. On the site Giessen, however, there were some cases in which the N fertilizer did not increase grain yield. The soil of the Giessen site was rich in interlayer NH₄⁺ which is not recovered by the EUF and Nmin method, but which obviously contributed to the N supply of the crop, and therefore the N rates were too high. Grain crude protein concentration were higher with Nmin for Wernborn and Bruchkobel sites because of higher N fertilizer rates. For the Giessen site in 1989/1990 the reverse was true. Nitrogen agronomic efficiency (AE) ranged from 0 to 35.6. Apparent N recovery (ANR) ranged from 0 to 111. The gross profit differed from −88 to 489 Euro/ha. Negative values (three cases out of 23) were found on the Giessen site where no yield increase was obtained by the N fertilizer because of interlayer NH₄⁺. This interpretation is supported by the finding that interlayer NH₄⁺ significantly decreased from autumn to spring. Apart from the results found in 1 year on the Giessen site, the gross profit calculation showed that a precise N fertilizer application based on soil analysis yields a high profitability of cereal production.     

Two contrasting laboratory methods improve <Emphasis Type="Italic">Silphium integrifolium</Emphasis> Michx. germination rate to agronomically acceptable levels

Organic dormancy, the inability to germinate under favorable conditions, is a common problem in many crop species and their wild relatives, leading to more variable emergence, plant density, and growth rates, thus increasing costs, and lowering yield. To overcome these problems, several different methods have been developed for various crop and model plants. However, in the emerging crop being bred from wild and semi-domesticated Silphium, no such method has been established thus far. The objective of this study was to identify a dormancy-breaking assay to increase the seed germination rate. Seeds of three different Silphium integrifolium Michx. genotypes were treated with five chemical and one cold treatment, and dried before or after the treatment at 27 °C or 40 °C. Untreated, dried seeds were taken as control. Seeds soaked for 24 h in a ethephon/potassium nitrate solution followed by a 72 h drying step at 40 °C showed an increase of germination to up to 90?±?2% compared to control seeds (3?±?0% and 5?±?1%). We identified the minimum time frame of cold stratification needed to enhance seed germination in Silphium, and found that cold stratification was nearly as good as the chemical treatment. Our results provide two alternate ways to treat S. integrifolium seeds for breaking organic dormancy, which will help to facilitate future research in the Silphium domestication community. The fact that our optimal treatments were similar to protocols developed for sunflower suggests that these methods may also be applicable to many related economically important Asteraceae species.     

EFFECTS OF CARBOHYDRATE SOURCE ON THE IN VITRO ASYMBIOTIC SEED GERMINATION OF THE TERRESTRIAL ORCHID HABENARIA MACROCERATITIS

The effects of the carbohydrates fructose, sucrose, and dextrose on the seed germination and protocorm development of the terrestrial orchid Habenaria macroceratitis were assessed in the presence or absence of banana powder. Malmgren Modified Terrestrial Orchid Medium was used as the basal medium. Each carbohydrate was screened at 50 mM with and without banana powder (15 g L^?1). No significant differences in germination or protocorm development among all treatments were observed after seven weeks. There were no significant differences in advanced development among carbohydrate treatments without banana powder after 21 weeks. However, the presence of banana powder in the medium suppressed advanced protocorm development in the fructose, dextrose, and control treatments. These data support the wide suitability of simple exogenous carbohydrates in supporting the germination and protocorm development of H. macroceratitis, and suggest that a wide range of carbohydrates are suitable for the culture of often difficult-to-germinate terrestrial orchids.