基于ANSYS的永磁电机转子冲片结构的优化设计

运用ANSYS的优化设计模块对永磁电机转子冲片隔磁桥附近的结构进行优化设计,采用APDL参数化设计通过拓扑形状优化功能确定冲片优化的初始区域,在此基础上运用尺寸优化功能确定最优的形状尺寸参数,最终结合工程化要求确定转子冲片的施工结构尺寸。电机最后通过最高转速试验表明文章运用的结构优化设计方法切实可行,满足工程化生产要求。     

Preparation and Identification of Canine Parvovirus NY Strain VP2 Protein Polyclonal Antibody

This study was aimed to prepare canine parvovirus (CPV) VP2 protein polyclonal antibody.The recombinant expression vector pET28a-CPV-VP2 was constructed and transfromed into E.coli BL21 (DE3),the expression of recombinant proteins was induced by IPTG from which the fusion protein was identified by SDS-PAGE.The target protein was purified and emulsify with adjuvant,the prepared immunogen was inoculated into rabbit by subcutaneous injections to prepare of VP2 protein specific polyclonal antibody.The immuno-activity,titers,neutralization titers of the prepared polyclonal antibody were determined by immunoperoxidase monolayer assay (IPMA).The results showed that the expressed recombinant protein VP2 (rVP2) existed in the form of inclusion body with a molecular weight of 72 ku.The prepared polyclonal antibody titer was 1 600 dilution,the virus titer was 10⁷ TCID₅₀/mL,the neutralizing titer was 1∶2 884.The antibodies showed specific reaction with CPV.In conclusion,rVP2 specific polyclonal antibody showed wonderful immunocompetence,specificity and neutralizing activity,providing foundation for the development of genetic vaccine and clinical therapeutic method.     

Effects of lncRNA-GTL2 Transgenosis on Gut Microbiota Composition in Mice

This research was aimed to study whether the lncRNA would have effects on the structure and constitution of the intestinal microbial colonies in mice.High throughout sequencing was used to sequence and analyze the intestinal microbial colonies in both transgenic and non-transgenic mice of three months old.We conducted comparison and t-test at the level of phylum and genus.The result showed that transferred into long non-coding RNA genes GTL2 (lncRNA-GTL2),the mouse intestinal microbial colony structure and composition had no significant differences in the overall,within the same gender,but there were individual differences.Therefore,this study didn't find that long non-coding RNA transfered had a significant impact on the intestinal microflorain the phylum and genus level.     

Isolation and Identification of Cellulase-producing Bacillus in Yak Rumen

To investigate the species of cellulase-producing Bacillus in yak rumen,10 samples of rumen content were aseptically collected from 10 adult Maiwa yaks to isolate the heat-resistant Bacillus by water bath at 80 ℃ for 20 min.The cellulase-producing strains were screened using the CMC-Na medium and Congo red staining.The 16S rRNA gene sequence of those cellulose-producing strains were amplified and sequenced.The results showed that 64 strains were isolated from the 10 samples.Total 23 strains were identified as cellulase-producing bacillus,including 16 strains of Bacillus cereus,7 strains of Bacillus thuringiensis.Furthermore,phylogenetic analysis showed that the 16 Bacillus cereus strains were clustered into two branches:One isolate was clustered into a branch alone,the other 15 isolates were clustered into a branch which clustered into 5 small branches,showing that there was certain genetic diversity in the isolates of Bacillus cereus.And all 7 Bacillus thuringiensis strains were clustered into a branch.Hence,the results layed the foundation of investigating the species of cellulase-producing Bacillus in yak rumen and developing probiotics special for yak.     

Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs

To study the expression pattern of THBS3 gene in different tissues and during skeletal muscle development, the THBS3 gene expression in different tissues and skeletal muscles during prenatal periods (33, 45, 65, 70 and 90 d) and postnatal periods (0, 9, 30, 60, 120 and 160 d) from Landrace and Tongcheng pigs were detected by Real-time quantification PCR.The results showed that THBS3 gene widely expressed in all tissues examined, exhibiting similar spatial expression patterns with expression peaks in lung in both pig breeds except in stomach and intestine.Moreover, although THBS3 gene showed a significant higher expression level in gestation than after birth in Landrace and Tongcheng pigs (P<0.05), it exhibited different expression patterns between Landrace and Tongcheng pigs, the expression peak was detected at gestation day 45 in Landrace pig, while was detected at gestation day 65 in Tongcheng pig.The results suggested that THBS3 gene involved in skeletal muscle growth and development in pigs, as well as the regulation of asynchronization of skeletal muscle development in different pig breeds.     

白檀未成熟胚的器官发生和植株再生

以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生.结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2 mg/L 6-BA+0.1 mg/L NAA的培养基中,诱导率高达92.5％;胚性愈伤组织分化最佳培养基为mMS+0.25 mg/L 6-BA+0.15 mg/L NAA,分化率为72.4％;分化出的胚性愈伤组织在空白mMS培养基上继代培养15 d,76.6％的组织分化出芽,再转入1/2mMS+ 1.5％蔗糖培养基上培养20 d,芽长至1.5 cm.     

基于叶绿体ndhA基因内含子序列的DNA条形码在秋海棠属物种鉴定中的应用

以18种秋海棠属植物为试材,基于叶绿体ndhA基因内含子片段,利用Codon Code Aligner软件拼接序列后,通过MEGA软件进行比对并计算19个秋海棠属植物种间及种内遗传距离,最后利用邻接法构建分子系统树,研究了该片段作为DNA条形码对秋海棠属植物进行物种鉴定的可行性。结果表明:秋海棠属19个种类共59个个体的ndhA基因内含子序列长度为1 182bp,在所考察的候选秋海棠属植物中具有最大的种间变异和较小的种内变异,且二者存在极显著差异。在系统树中,秋海棠属植物每一物种能够分别形成各自独立的分支,表现出良好单系性。基于ndhA基因内含子的DNA条形码在识别秋海棠属植物物种方面和传统形态学基本一致。该研究表明,以ndhA基因内含子作为秋海棠属植物DNA条形码进行物种鉴定具有一定的可行性。     

不同品种芹菜品质指标测定及其聚类分析

以12个不同品种的芹菜为试材,对其可溶性糖、可溶性固形物、叶绿素、维生素C、硝酸盐、纤维素、蛋白质、氨基酸、微量元素、芹菜素含量等10个营养品质性状进行了测定,采用聚类分析对其进行比较。结果表明:不同品种的芹菜在营养品质方面不尽相同,其中"鲍芹"营养成分丰富,维生素C、芹菜素含量极高,适合食用。试验初步明确了各品种芹菜营养成分,为进一步开发芹菜的应用价值提供理论依据。     

棉铃虫 JNKs 基因的克隆及表达分析

c-Jun氨基末端激酶(c-Jun N-terminal kinase, JNK)是MAPK家族(mitogen-activated protein kinases, 丝裂原活化蛋白激酶)的重要成员, 具有参与昆虫抗逆反应等多种功能。为明确JNK在棉铃虫 Helicoverpa armigera 中的表达特性及其对Bt杀虫蛋白的应激与免疫反应, 本研究通过PCR克隆得到2个棉铃虫 JNK 基因 HaJNK1 和 HaJNK2; 生物信息学分析结果显示: HaJNK1和 HaJNK2基因开放阅读框分别为1 191、1 143 bp, 分别编码397、381个氨基酸。系统进化树分析结果表明棉铃虫 HaJNK1 与黏虫 Mythimna separata 聚为一支, 亲缘关系较近, HaJNK2与 家蚕 Bombyx mori 聚为一支, 同源性较高。利用实时荧光定量PCR技术分析 HaJNK1 与 HaJNK2 在棉铃虫不同发育时期、不同组织中的表达量, 发现 HaJNK1 与 HaJNK2 在卵中表达量最高, 其次是雌成虫; HaJNK1 在性腺中表达量最高, 其次是唾液腺; HaJNK2 在头部表达量最高, 其次是性腺。取食Cry1Ac的4龄棉铃虫幼虫的中肠组织中, HaJNK1 与 HaJNK2 的表达量均显著升高。推测 HaJNKs 基因可能参与棉铃虫抵御Bt杀虫蛋白伤害的应激和抗逆反应。     

农牧交错带灌溉和旱作模式下马铃薯耗水差异

为揭示北方农牧交错带地区马铃薯“水改旱”种植对其产量和水分利用的影响,选取中国北方农牧交错带地区27个站点,基于站点的气象数据、土壤数据和管理数据驱动充分验证的APSIM-Potato模型,模拟分析农牧交错带地区灌溉和雨养马铃薯的耗水差异。结果表明：北方农牧交错带地区马铃薯种植连续灌溉10、20 a和30 a的产量分别为15 900～35 600、16 400～34 800 kg·hm^-2和16 600～34 800 kg·hm^-2,改为旱作后对应的产量分别为12 800～30 600、13 900～29 100 kg·hm^-2和12 700～25 500 kg·hm^-2;灌溉马铃薯产量均表现为西部较高,旱作马铃薯产量则为东部较高。连续灌溉10、20 a和30 a的播前1 m土层土壤含水量分别为163～388、161～394 mm和154～398 mm,改为旱作后分别下降31.8%、35.3%和36.9%。连续灌溉10、20 a和30 a后的地下水消耗量分别为5 360～21 330、8 910～4...