基于叶绿体ndhA基因内含子序列的DNA条形码在秋海棠属物种鉴定中的应用

以18种秋海棠属植物为试材,基于叶绿体ndhA基因内含子片段,利用Codon Code Aligner软件拼接序列后,通过MEGA软件进行比对并计算19个秋海棠属植物种间及种内遗传距离,最后利用邻接法构建分子系统树,研究了该片段作为DNA条形码对秋海棠属植物进行物种鉴定的可行性。结果表明:秋海棠属19个种类共59个个体的ndhA基因内含子序列长度为1 182bp,在所考察的候选秋海棠属植物中具有最大的种间变异和较小的种内变异,且二者存在极显著差异。在系统树中,秋海棠属植物每一物种能够分别形成各自独立的分支,表现出良好单系性。基于ndhA基因内含子的DNA条形码在识别秋海棠属植物物种方面和传统形态学基本一致。该研究表明,以ndhA基因内含子作为秋海棠属植物DNA条形码进行物种鉴定具有一定的可行性。     

不同品种芹菜品质指标测定及其聚类分析

以12个不同品种的芹菜为试材,对其可溶性糖、可溶性固形物、叶绿素、维生素C、硝酸盐、纤维素、蛋白质、氨基酸、微量元素、芹菜素含量等10个营养品质性状进行了测定,采用聚类分析对其进行比较。结果表明:不同品种的芹菜在营养品质方面不尽相同,其中"鲍芹"营养成分丰富,维生素C、芹菜素含量极高,适合食用。试验初步明确了各品种芹菜营养成分,为进一步开发芹菜的应用价值提供理论依据。     

坡度和采挖强度对玉树冬虫夏草幼虫数量和采挖数量的影响

【目的】研究人工采挖和自然坡度对冬虫夏草数量的影响。【方法】通过对不同采挖强度 S₁ (2 人/hm² )、S₂ (4 人/hm² )和 S₃ (6 人/hm² )和产区自然坡度(G₁,7°;G2,17°;G3,25°)下冬虫夏草幼虫数量和采挖数量进行调查,分析采挖强度和产区坡度对幼虫数量和冬虫夏草采挖量的影响。【结果】采挖强度和坡度与幼虫数量和冬虫夏草采挖数量均呈显著负相关关系(P＜0. 05);采挖强度和幼虫数量相关度高(r ² =-0. 45,r ² =-0. 58),坡度和冬虫夏草采挖数量相关度高(r ² =-0. 74,r ² =-0. 78),采挖强度和坡度互作对幼虫数量和采挖数量影响不显著(P＞0. 05)。不同采挖强度下,幼虫数量和冬虫夏草采挖数量均为 CK=S₁＞S₂＞S₃( P＜0. 05);不同坡度下,幼虫数量和冬虫夏草采挖数量均为 CK=G₁＞G₂＞ G₃( P＜0. 05);且在各个采挖强度下,随坡度的增加,幼虫数量和冬虫夏草采挖数量均呈显著降低趋势 (P＜0. 05)。 此外 ,冬虫夏草采挖数量显著地受蝙蝠蛾幼虫数量的影响且呈正相关(P＜0. 05)。 【结论】 S₁采挖强度、G₁小坡度适合幼虫数量的增长和冬虫夏草的采挖数量,且不影响翌年冬虫夏草的产量。然而,采挖活动有可能对草地的土壤、幼虫喜食性牧草、冬虫夏草真菌孢子的弹射等产生综合影响,土壤的理化性质会影响蝠蛾寄主幼虫的存活状态,冬虫夏草菌孢子的弹射量也会影响冬虫夏草的形成。     

Test Analysis of Ecosystem on Functional Groups of Microorganism in Spiral Up- flow Reactor System

The test of functional groups of microorganisms in Spiral Up-flow Reactor System and the correlation between microorganisms in the water and the main environmental factors are studied.The results show that the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable.The close relations between abundance of bacteria and nutrients are found.The correlation between heterotrophic bacteria and COD is 0.949.The correlation between organic phosphate bacteria and TP is 0.815. The correlation between nitrosobacteria and NH_3-N is 0.909. The correlation between disnitrifier bacteria and TN is 0.653.     

A Furry Synthetic Evaluation Model in Capital Structure Decisions

This paper analyzes the limitation of regarding the weighted average cost of capital as the optimal standard of capital structure in the traditional capital structure decision-making method, and analyzes the view that the capital structure is affected by many factors,such as earning power, long-term stability of operating, the attitudes held by owners and operators, the attitudes held by creditors and financial risk. With the example, the affecting factors of the capital structure is analyzed. The fuzzy synthetic evaluation model are employed to decision-making of the capital structure. This article also shows the key point of its application.     

Study on Effects of the Reaction Conditions on Properties of Poly(aniline-co-o-anisidine)

The solubility and processability of polyaniline can be increased through aniline copoly with o-anisidine. Poly(aniline-co-o-anisidine) have good electrochromism .On condition that mole ratio between aniline and anisidine is 3:1,better reaction conditions of synthesizing poly(aniline-co-o-anisidine) with good solubility ,electrochromism and high yield was gotten through researches for effects of monomer ratio,monomer concentration,the amount of oxidant ,reaction times,reaction temperature,acid concentration on properties of poly(aniline-co-o-anisidine).The structure of polymers were characterized by IR spectrum.     

Pd Pattern Recognition Based on Linear Discriminant Analysis in GIS

According to the character of PD in GIS, the authors design four kinds of GIS defection models. The GIS gray intensity images are constructed based on mass specimens gathered by the ultra - high frequency and high speeds systems, Aiming at the PD characteristics and its defections, A PCA-FDA method is put forward based on PD images. The principal component analysis is employed to condense the dimension of PD images, then the optimal sets of statistically uncorrelated discriminant vectors are extracted, and the minimum distance classifier is constructed as classifier. The identified results show that this method can effectively elevated the discrimination of the four kinds of defects in GIS PD.     

Work-hardening Mechanism of Non-magnetic High Manganese ZG25Mn18Cr4 Castings

The important character of non-magnetic high manganese ZG25 Mn18Cr4 alloy is rapid work-hardening under external stress. With X-ray diffraction and TEM analysis, it is proved that the microstructures of both undeformed and deformed matrixes are austenite and carbide with some striation structure in the matrixes, the amount of stripes in the deformed matrix is obviously more than that in the undeformed matrix. Electronic diffraction analysis confirmed that the stripes in coarse austenite grains are high density lamination fault sheets. A great deal of lamination fault sheets produced by plastic deformation divides up austenite matrix, shortens the free distance of dislocation movement and results in the rapid work-hardening of ZG25Mn18Cr4 alloy.     

Land Storage System: Effect, Problem and Its Legal Regulation

The authors suggest that the land storage system is beneficial not only to strengthen the country's macroscopic regulation of city land market, accelerate healthy development of city economy and increase the financial income, it can also be advantageous to carry out the economic reform and public facilities construction without a hitch. On the basis of this, in this paper, the increment of the land price and house price as well as the high risk of fund due to lack of related laws is analyzed. Corresponding legal regulations, such as polishing the legislation of land right, implementing the land utilization planning, reinforcing the legislation of city development, land requisition and countryside residential land administration are put forward.