丹东市林业资源综合分析

分析了丹东市林业资源现状;指出了目前存在的主要问题;提出了合理开发利用林业资源的措施。     

Effects of FK506 on anti-glomerular basement membrane nephritis in rats

AIM:To investigate the effects of FK506 on anti-glomerular basement membrane(GBM) nephritis in rats. METHODS: Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody injection in SD rats in this study. The rats were divided into three groups: FK506 treated group(0.5 mg·kg^-1·d^-1, sc), untreated nephritis control group and normal control group. FK506 was administered daily six hours after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected, and T cell transforming function was also monitored.RESULTS:Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4 days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephritic rats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. T cell transforming function was higher than normal. But, all pathological changes obviously turned for the better in FK506 treated group. CONCLUSION:FK506 could inhibit the progression of rat anti-GBM nephritis.     

Preparation of gfp-bcl-XL-contained recombinant adenovirus vector by the homologous recombination in bacteria

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.     

蛋鸡血浆VLDL浓度二世代双向选择对体脂沉积的影响

以雅发褐壳蛋鸡为素材,在零世代和一世代双向选择的基础上,应用血浆极低密度脂蛋白(VLDL)浓度对体腹脂性状继续进行双向选择,比较了高低脂系间体组成的差异,并研究了中后期血浆VLDL浓度与上述性状的相关程度,进一步探讨了血浆VLDL浓度的选择效应。结果表明:二世代母鸡中后期高脂系体重较高(P<0.05),血浆VLDL浓度与体重呈极显著的表型相关(P<0.01)和中等程度的正遗传相关。62周龄高脂系的腹脂率显著高于低脂系(P<0.05),说明运用血浆VLDL浓度经过二个世代的双向选择,使产蛋鸡中后期体重降低,且对腹脂沉积产生了一定的影响。     

紧凑型杂交玉米新品种毕玉2号的选育与应用

毕玉2号系毕节地区种子站用自选系苏110作母本,自选系毕106作父本育成的白粒紧凑型玉米杂交种,2000年11月通过贵州省毕节地区农作物品种审定委员会审定,具有较强的耐旱耐瘠能力和品质优良等特点,适宜在毕节地区海拔1 800 m以下及其他气候相似地区的旱坡地及肥水条件好的坝地种植,目前正在生产上大面积推广应用。     

逻辑反函数的特殊表示形式的应用

给出了形式为F=∑Iimi的逻辑函数,其反函数为F=∑Iimi,利用这种表示反函数的方法,可简化一些逻辑等式的证明过程,并可使一些逻辑电路的设计过程简化。     

老年居住建筑的门户空间景观研究

目的改变老年居住建筑门户空间的使用现状,应对老龄化社会带来的老年人居住问题．方法通过对相关老年居住建筑门户空间的理论研究和其功能、环境特点的分析,用POE的研究方法对使用现状的评价做出分析．结果最后得出设计成功的老年居住建筑门户空间在景观设计上应注意的三个基本要求：建筑物、大门、地面铺装．结论缺少安全感和交往空间的门户空间对老年人的心理、生理上都有很大的影响,通过景观设计改进老年居住建筑门户空间的利用现状势在必行．     

小麦籽粒发芽对品质的影响

选用来自黑龙江、辽宁和北京的11个冬、春性小麦品种,对其发芽籽粒和耒发芽籽粒进行了11个品质性状的分析,以研究籽粒发芽对小走品质性状的影响。结果表明,小麦籽粒发芽对其蛋白质、淀粉和馒头加工品质均造成负面影响,籽粒发芽与不发芽相比,蛋白质舍量、湿面筋含量、沉淀值、吸水率、形成时间、稳定时间、峰值粘度、稀懈值、降落值、馒头体积和馒头评分等品质性状的差异均达到5％或1％显著水平。籽粒发芽造成蛋白质质量的下降程度大于蛋白质含量的下降程度。籽粒发芽对品质影响程度在品种（系）间也存在差异。发芽处理的小走高分子量走谷蛋白亚基组成和醇溶蛋白电泳图谱均未发生变化。     

记录小麦SSR带型的快捷方法

针对小麦SSR带型识别和记录中因带纹繁多和分布复杂而造成带纹判读和记录困难的特点,提出了根据每对SSR引物生成带型间的差别直接对带型进行编号,以有效简化小麦SSR带型的记录方法。该方法避免了由于错误判断引起的实验误差,并可利用每个品种的带型编号,建立品种的DNA指纹以及进行品种基因型比对。     

水、氮、钾互作对砂田嫁接西瓜产量和品质的影响

【目的】西北砂田嫁接西瓜生产中水肥管理不当常造成品质下降。研究采用膜下滴灌栽培模式,水、氮、钾三因素组合对西瓜产量和品质的影响,为西北砂田嫁接西瓜高产、优质生产提供理论依据。【方法】以中晚熟西瓜品种‘金城5号’为供试材料进行微区试验。以水(W)、氮(N)、钾(K)为3个主因素,采用二次饱和D-最优设计确立了3因素5水平试验,共11个处理。测定西瓜产量和品质,分别建立产量、平均含糖量与水肥用量二次回归拟合数学模型,并计算产量和品质目标下的水肥用量。【结果】主因子效应分析表明,对西瓜产量的影响顺序为W>N>K,水对产量的正效应和氮对产量的负效应均极显著,钾对西瓜产量影响不显著。对西瓜品质的影响顺序为N>K>W,氮、钾对西瓜品质的正效应和水对西瓜品质的负效应均达到显著水平。对西瓜产量的耦合效应分析表明,交互效应表现为N×W>N×K>W×K,W×K的交互效应大于氮的单因素效应。对西瓜品质的耦合效应结果为W×K>W×N>N×K。产量与水肥回归模型计算得出,西瓜产量超过60 t/hm²的W、N、K组合方案为灌水量808～1017...