Evidence of breed-dependent differences in susceptibility to porcine circovirus type-2-associated disease and lesions

Porcine circovirus type 2 (PCV2) has been confirmed as the primary cause of postweaning multisystemic wasting syndrome (PMWS). However, in the field, PMWS is seen only in a small percentage of pigs infected with PCV2. The overall objective of the study reported here was to determine whether host genetic differences in the susceptibility to PCV2-associated disease exist among selected breeds of pigs. This study included Duroc (n = 23), Landrace (n = 19), and Large White (n = 21) pigs. The pigs were infected intranasally and intramuscularly at 5-7 weeks of age with PCV2. A portion of the pigs (31/63; 30.2%) had low passively acquired PCV2 antibodies at the time of infection. There were no differences in mean weight gain, rectal temperature, or respiratory score. Clinical disease compatible with PMWS was observed only in the Landrace pigs. Most of the PCV2-infected pigs had enlarged lymph nodes, and individual Duroc and Landrace pigs had mottled tan lungs. PCV2-associated lymphoid depletion and granulomatous inflammation were observed in pigs of all breeds. Three of 19 Landrace pigs and none of the Duroc or Large White pigs developed severe lymphoid lesions associated with large amounts of intralesional PCV2 antigen typical of PMWS. Compared with seronegative Landrace pigs, Landrace pigs that had low maternal antibodies at the time of PCV2 inoculation had significantly (P < 0.05) less-severe PCV2-associated lesions. The results suggest a predisposition of the Landrace pigs of this study to PCV2-induced disease and lesions, and that low levels of passively acquired antibodies are protective.     

Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells

The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next‐gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead (Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources of the CCO cell line and all matched the brown bullhead and not the channel catfish.     

Comparison between 10,000-year old and contemporary spruce lignin

Wood from white spruce Picea glauca that had been preserved by rapid burial in lake sediments 10,000 years ago, was investigated and compared to a contemporary reference white spruce wood. The 10,000-year old sample appeared to have an intact primary cell wall and middle lamella, whereas the carbohydrate monomer distribution, and microscopic images showed that the secondary wall was at least partially removed, indicating that this structure had been selectively attacked by bacteria. The Klason lignin amount in the aged spruce was found to be 60%. The relative lignin monomer content in the aged spruce was 9% lower than that of the reference wood, showing that there were fewer β-O-4′ linkages in the aged sample. This finding was supported by SEC analysis of the thioacidolysed samples as a larger proportion of lignin oligomers were observed in the aged spruce than in the reference material. This indicates a somewhat greater number of condensed bonds in the aged spruce than in the reference spruce sample. Quantitative ¹³C NMR analysis and HSQC techniques applied on milled wood lignins (MWL) revealed no significant structural differences between the aged spruce and the reference.     

Farmlink: promoting conservation buffers farmer-to-farmer

Farmer-to-farmer outreach was used within a targeted watershed to promote the installation of conservation buffers. In this program called “FarmLink”, four farmers/landowners were employed part-time as “advisors” and trained by University of Nebraska-Lincoln Extension, Natural Resources Conservation Service, and Natural Resources District personnel. Topics included basic buffer design and benefits, availability of incentive programs, and sales techniques. These individuals then contacted their neighbors to explain the need for and benefits of buffers and other conservation practices. In early 2003, 42 landowners were contacted, leading to contracts for the establishment of 16 separate conservation practices on 24.8 ha (61.3 acres) of farmland. These included just over 8 ha (20 acres) of grassed waterways or similar plantings and 14 ha (35 acres) of streamside buffers. In addition, because of information received in the training sessions, one of the advisors installed 1.7 ha (4.3 acres) of streamside buffers, 0.45 ha (1.1 acres) of grassed waterways, and 2.0 ha (5.0 acres) of grasses and forbs on his own land. During these contacts, it became apparent that: (1) many farmers and landowners were not familiar with the multitude of programs available to assist with the installation and maintenance of conservation practices; and (2) landowners generally appreciated the personal touch of someone coming out to talk directly to them, pointing out specific areas on their land where conservation practices could best be implemented, discussing available compensation programs, and describing management needed to help ensure practice success. Although one-to-one contacts cannot be used in all cases, it was demonstrated to be effective in this watershed.     

Disturbance history and dynamics of an old-growth mixed species mountain forest in the Slovenian Alps

A long history of human presence in the Alps has made studies of natural forest structure, composition, and disturbance processes difficult. In the Slovenian Alps, we identified a mixed European beech (Fagus sylvatica L.)-silver fir (Abies alba Mill.)-Norway spruce (Picea abies (L.) Karst.) stand with old-growth characteristics that escaped past cutting. We reconstructed the dynamics of the stand using a combination of stand and age structure analyses, dendroecological evidence of past canopy disturbance, and historical evidence of past human activities. The disturbance reconstruction revealed a stand-scale disturbance in the 1850s. Based on the presence of pit-and-mound topography throughout much of the stand and the absence of logging activities found in historical records, we assume this event was caused by strong winds. The current structure and composition of the stand are largely a result of this event. Post-disturbance forest development was dominated by the release of shade tolerant regeneration, especially F. sylvatica. The windthrow also created appropriate conditions (i.e. increased light and exposed mineral soil) for recruitment of less shade tolerant species, including larch (Larix decidua Mill.) and maple (Acer pseudoplatanus L.). The results suggest that periodic, intermediate severity wind disturbances, similar to the event documented here, may have had an important influence on pre-settlement forest dynamics in mixed mountain forests of the Alps, and add to the limited available information regarding the historic range of natural variability of disturbance processes. This information may be useful for forest management that attempts to emulate natural processes.     

Response of soricid populations to repeated fire and fuel reduction treatments in the southern Appalachian Mountains

Fuel hazards have increased in forests across the United States because of fire exclusion during the 20th century. Treatments used to reduce fuel buildup may affect wildlife, such as shrews, living on the forest floor, especially when treatments are applied repeatedly. From mid-May to mid-August 2006 and 2007, we used drift fences with pitfall traps to capture shrews in western North Carolina in 3 fuel reduction treatment areas [(1) twice-burned (2003 and 2006), (2) mechanical understory cut (2002), and (3) mechanical understory cut (2002) followed by 2 burns (2003 and 2006)] and a control. We captured 77% fewer southeastern shrews (Sorex longirostris) in mechanical + twice-burned treatment areas than in mechanical treatment areas in 2006, but southeastern shrew captures did not differ among treatment areas in 2007. Total shrew captures did not differ among treatment areas in either year. Decreases in leaf litter, duff depth, and canopy cover in mechanical + twice-burned treatment areas may have decreased ground-level moisture, thereby causing short-term declines in southeastern shrew captures. Prescribed fire or mechanical fuel reduction treatments in the southern Appalachian Mountains did not greatly affect shrew populations, though the combination of both treatments may negatively affect some shrew species, at least temporarily.     

高压静电喷雾纯天然有机制剂"泰栢一号"对蔬菜蚜虫的防治效果

"泰栢一号"是一种纯天然有机制剂,本文以吡虫啉为对照药剂,分别在室内与田间测试了在常规使用和在高压静电喷雾条件下"泰栢一号"对蔬菜蚜虫的防治效果,结果表明,"泰栢一号"40X稀释液处理蚜虫48h后死亡率为3.36%,清水对照处理的死亡率为3.33%,二者间无显著性差异,而吡虫啉10 mg/L处理24h蚜虫的死亡率为78.86%."泰栢一号"与高压静电喷雾器配合使用则表现出良好的杀虫效果,200×稀释液处理48h的蚜虫死亡率为62.22%,96h蚜虫死亡率为91.1%,这种纯天然有机物结合高压静电喷雾杀虫方法的发现,为蚜虫的无公害防治提供了一条新的途径.     

An analysis of a community food waste stream

Food waste comprises a significant portion of the waste stream in industrialized countries, contributing to ecological damages and nutritional losses. Guided by a systems approach, this study quantified food waste in one U.S. County in 1998–1999. Publications and personal interviews were used to quantify waste from food production, processing, distribution, and consumption. Approximately 10,205 tons of food waste was generated annually in this community food system. Of all food waste, production waste comprised 20%, processing 1%, distribution 19%, and 60% of food waste was generated by consumers. Less than one-third (28%) of total food waste was recovered via composting (25%) and food donations (3%), and over 7,000 tons (72%) were landfilled. More than 8.8 billion kilocalories of food were wasted, enough to feed county residents for 1.5 months. This case study offers an example of procedures to quantify and compare food waste across a whole community food system.     

Enhanced detection of infectious salmon anaemia virus using a low‐speed centrifugation technique in three fish cell lines

Infectious salmon anaemia (ISA), caused by ISA virus (ISAV), is a serious disease of farmed Atlantic salmon, Salmo salar L. Recently, molecular‐ and immunofluorescent‐based techniques have become powerful diagnostic tools for ISAV detection, but culture‐based techniques remain the gold standard. A disadvantage of ISAV culture is that the incubation time required before cytopathic effect (CPE) is observed in cell monolayers. To decrease time until CPE is observed, a low‐speed centrifugation technique was applied to existing standard operating procedures for ISAV culture in three fish cell lines. Time until CPE observation was compared in CHSE, SHK and ASK cells, treated or not treated with low‐speed centrifugation after inoculation with ISAV. Low‐speed centrifugation treatment significantly enhanced observable cell infection. Compared to control cells, the length of time until ISAV CPE observation decreased in centrifuged ASK and CHSE cells. Low‐speed centrifugation was also incorporated into a modified clinical shell vial assay. At 48 h post‐inoculation with approximately 20 viral particles, ISAV was detected by an immunofluorescence antibody test in treated ASK and SHK1 cells but not in control cells. Finally, this enhanced viral adsorption assay performed in ASK cells demonstrated higher sensitivity than a real‐time RT‐PCR assay performed on RNA isolated from ISAV‐spiked salmon kidney homogenates.     

Effect of water hardness on peracetic acid toxicity to zebrafish, Danio rerio, embryos

The use of peracetic acid (PAA) in aquaculture has been suggested as an alternative therapeutic agent. Few data are available concerning fish toxicity by PAA or factors that modify this toxicity. The aim of this study was to investigate the influence of water hardness on the acute toxicity of PAA products to embryos of zebrafish (Danio rerio). Embryos were exposed to PAA ranging from 0 to 9 mg/L in low-hardness (1.4 °dH or 25 mg/L hardness as CaCO₃), medium-hardness (14 °dH or 250 mg/L hardness as CaCO₃) and high-hardness (140 °dH or 2,500 mg/L hardness as CaCO₃) waters. The lowest LC50 value was 2.24 mg/L PAA in the low-hardness water, and the highest LC50 value was 7.14 mg/L PAA in the high-hardness water. Toxicity of PAA to embryos was found to be negatively correlated with water hardness. The pH decreased with increasing concentrations of PAA, and the test waters were observed to become more acidic in low hardness. In conclusion, aquaculturists using PAA should pay attention to water hardness to avoid acidosis.