长期冻存对昆虫细胞系SL2和NIH-SaPe-4活性的影响

对2种双翅目昆虫细胞系(黑腹果蝇胚细胞系SL2以及麻蝇胚细胞系NIH-SaPe-4)38个月长期液氮深低温保存效果进行了研究,通过对冻存时间和保护剂对2种细胞系冻后活力、圆度以及恢复时间影响的测定,比较了3种不同配方冻存保护液的效果。结果表明:2种昆虫细胞系冻后活性均随着冻存时间的增长而逐渐降低。长期冻存期中,使用90%胎牛血清(FBS)+10%二甲基亚砜(DMSO)冻存SL2效果较好,NIH-SaPe-4使用10%DMSO即可满足冻存的需要,而无需额外添加高浓度FBS。甘油不适用于这2种昆虫细胞系的长期保存。     

电容式植物含水率测量传感器的数学建模及电路设计

通过对平行板电容传感器工作原理的研究，提出在植物体含水率测量中将电容极板间被测物质和间隙空气抽象为固、液、气三相的方法，理论上分别建立用于植物水分测量的三相介质的并联、串联数学建模，并做数值模拟分析，得出平行板电容传感器可以实现直接针对植物含水率测量的结论，解决间接测量的滞后性和准确性差的问题，确定三相介质并联可以作为传感器系统理论分析的最佳方案？     

籽粒苋类II型金属硫蛋白基因的分离及其表达分析

Metallothioneins (MTs) are a group of small,Cys-rich, metal-binding proteins. Plant MTs are divided into two types based predominantly upon the arrangement of Cys residues: those only containing the Cys-Xaa-Cys (Xaa is an amino acid other than Cys)metal-binding motif (type 1) and those containing a combination of the Cys-Cys,Cys-Xaa-Cys and Cys-Xaa-Xaa-Cys motifs (type 2).Both type 1 and type 2 contain two Cys-rich MT-like amino- and carboxyl-terminal domains separated by a central region of about 40 residues which is devoid of Cys (Robinson et al.,1993).Here we report a type 2 MT from amaranth.     

Effect of bFGF on glucose regulated protein 78 expression and apoptosis in human ovarian cancer CAOV3 cells

AIM: To evaluate the effect of bFGF on the apoptosis and the expression of glucose regulated protein 78 (GRP78) in ovarian cancer CAOV3 cells.METHODS: Starvation induced cell apoptosis was conducted. After treatment with bFGF, the cell cycle and apoptosis were determined by FACS analysis and Annexin V/PI staining, respectively. The expression of protein kinase B (PKB) and GRP78 were detected by Western blotting and RT-PCR. RESULTS: Compared to starvation group, the cells treated with bFGF were still viable and increased activation of PKB and high expression of GRP78 were observed, which were prevented by PKB inhibitor wortmannin effectively (P<0.01). CONCLUSION: bFGF plays a critical role in anti-apoptosis and proliferation in human ovarian cancer through PKB signal transduction pathway, partly by upregulating the expression of GRP78.     

舟曲县“长治”工程建设推行项目公示制的做法及效果

舟曲县在"长治"工程建设中,针对农村"两工"取消、其他建设项目投资标准较高、工程建设发动群众困难和工程建设进展缓慢、建设质量不高等实际问题,推行了"长治"工程建设项目公示制,采用了事前公示、事后公示,做到了公开、公平、公正,使广大群众对水土保持建设项目有了充分的了解,极大地调动了群众投入水保生态建设的积极性,有效地推动了"长治"工程建设向"高、深、细"方向发展。介绍了推行项目公示制的做法及效果。     

不同含水率对谷子籽粒压缩力学性质与摩擦特性的影响

为了探明不同含水率谷子籽粒的物理机械性质,减少谷子籽粒在播种、碾米加工及储运等过程中受到压缩载荷及摩擦而产生的机械损伤,该文针对不同含水率的谷子籽粒进行压缩力学性质与摩擦特性试验。研究了谷子籽粒的挤压破碎过程,获得不同含水率谷子籽粒的力-位移（变形）曲线,破坏力、变形量及破坏能。随着含水率升高,破坏力减小,变形量和破坏能呈现先降低后升高的变化规律。同时采用赫兹接触理论,得到谷子籽粒单向表观弹性模量和许用挤压应力,结果表明二者都随含水率升高线性降低。分别测定了谷子籽粒与钢板和铝板间的滑动摩擦系数,随含水率升高,谷子与该2种材料的摩擦系数均增大,且与铝板的摩擦系数要高于钢板。根据试验结果,分别拟合得到了压缩和摩擦力学性能指标与谷子含水率的关系方程,为谷子播种、仓储、加工等装备设计及参数优化提供了基础依据。     

可控整流缩短连续极永磁发电机电压恢复时间

连续极永磁型无刷直流发电机是具有梯形波电动势的混合励磁发电机。为了缩短电压恢复时间,该文提出可控整流方法,利用电机电枢绕组实现能量快速存储与释放,通过提高电枢绕组电动势加快电压恢复速度,并且不会增加硬件成本。采用状态空间平均法分析了超级电容储能的可控整流系统电压调节过程,完成了可控整流与二极管整流方式下电压恢复时间的理论计算和试验。试验结果表明:与二极管整流相比,可控整流能将电压恢复时间减少约一半。该研究对提高车载敏感电子设备工作的可靠性提供了参考。     

Very low vitamin C activity of orally administered L-dehydroascorbic acid.

The biological activity of L-dehydroascorbic acid (DHA), which is easily formed from L-ascorbic acid (ASC) during storage and cooking processes, has been considered to be equivalent to that of ASC on the basis of studies made several decades ago, when a specific method to determine ASC was not available. The nutritional activity of orally ingested DHA has now been evaluated by comparing ASC concentrations in 12 tissues of rats administered four different doses of ASC. Determinations were made by using the specific and sensitive method, which had been developed by us. Here it is shown that the efficiency of DHA was almost 10% of that of ASC on a molar basis, based on animal experiments using the inherently scorbutic ODS rat, which is a convenient human model animal to investigate the metabolism of vitamin C. On the basis of these findings, it is proposed that it is necessary to reevaluate the nutritional requirement of vitamin C based on both ASC and DHA contents of foods.     

基于计量地理模型的松嫩高平原土地利用特征及其空间布局

研究土地利用特征及其空间布局是实现区域土地合理开发和利用的基础。该文以松嫩高平原典型地域黑龙江省巴彦县为研究区,以2006年巴彦县土地利用变更调查矢量数据库为基础,运用计量地理模型和现代地理信息空间分析技术相结合方法,探讨使用土地利用总体组合类型、土地利用综合程度及各地类的区位意义,来表征巴彦县18个乡镇土地利用的数量特征,进而分析其空间布局特征等。结果表明:研究区土地利用的数量特征总体存在一定的差异性,土地利用组合类型表现为土地整体功能较弱,土地利用程度高低不一,各地类中耕地和水域的区位意义较为突出;空间格局的总体特征表现为各地类斑块的形状总体较为复杂,其中耕地、林地的形状较规则且分布集中连片,为该区域的景观基质,未利用地、其他农用地和园地的分布最为离散。     

一种可分解式采集土壤整段标本的木盒装置设计

本文中介绍了一种可分解式野外采集土壤整段标本的木盒装置及野外具体操作使用流程。对比目前常用的标本采集装置,有如下优点:(1)上、下盖面板可以互用,简化了装置的制作过程,对于室内进一步制作标本而言,可以有效选择更加适合的一面作为土壤观察面,使用灵活;(2)装置的固定措施更加简易、快捷,固定得也更加牢固,整个过程不需使用钉子,也使操作人员免于受伤;(3)装土框与上、下盖面板可自由拆解,也容易拆解;(4)结构简单,制作简易、快捷,成本低廉,可重复利用。