秸秆类生物质酶解动力学与光合生物产氢特性研究

采用类分形动力学对4种常见秸秆类生物质的酶水解过程及其光合生物产氢能力进行了实验研究,并分析了各种秸秆类生物质的光合生物产氢能力及其与产氢菌种生长之间的相关关系,得到了秸秆类生物质酶解及光合生物产氢的相关动力学方程。实验结果表明,4种秸秆类生物质的酶解效果与产氢能力从大到小依次为小麦秸秆、玉米秸秆、高粱秸秆、棉花秸秆,酶解后还原糖质量浓度分别达到了19.88、15.72、14.04、9.41 g/L,累积产氢量分别达到了515.7、362、194.8、123.95 m L,且在菌种生长的对数期产氢速率达到最大。同时,利用类分形动力学揭示了秸秆类生物质酶解动力学参数与还原糖质量浓度及累积产氢量成正比例关系,为进一步完善秸秆类生物质光合生物产氢工艺理论和技术提供了参考。     

基于切比雪夫多项式的数控机床几何误差参数化建模

为了快速精确地建立机床几何误差项数学模型,提出了一种基于切比雪夫多项式的参数化建模方法。首先针对测量得到的机床基本几何项数据,将机床相应运动轴进给量转化为切比雪夫变量。其次将切比雪夫变量代入不同阶次的切比雪夫多项式得到相应的值。然后根据切比雪夫基函数值和切比雪夫变量用多元线性回归方法获得相应的系数,得到关于切比雪夫基函数的数学模型。最后将运动轴进给量与切比雪夫变量之间的转化关系代入得到基本几何误差项的数学模型。建模过程简单且易程序化,切比雪夫多项式的高逼近精度使得建立的模型精度高。将所有几何误差项参数化模型代入机床几何误差模型综合数学模型,从而可得到机床工作空间几何误差场分布。以MV-5A三轴立式加工中心为例,将各个几何误差项参数化模型代入机床几何误差模型中得到该机床综合几何误差数学模型,进而得到该机床工作空间几何误差场分布,为机床设计和误差补偿提供了理论依据。     

能量高效的农田无线传感器网络拓扑关联路由算法

针对农田无线传感器网络节点分布不均、能量约束严格等特点,提出了一种能量高效的簇头选择方法TBCS-EA,在以剩余能量进行簇头选择的基础上根据节点拓扑位置、拓扑密度等进行加权,使距离sink较近的节点与密集区节点大概率成为簇头,提高成簇能量使用效率。针对现有成簇算法频繁进行簇头选举,算法开销大等问题,提出了一种能量逼近式簇头轮换机制,节点连续担任簇头并以某一目标进行能量逼近,在达到逼近目标后进行根据簇内信息指定新簇头,减少簇头选择的次数与协议开销。仿真与实验结果表明,TBCS-EA算法的网络生命周期约为LEACH的2.2倍,CHCS的1.5倍,从节点能量曲线看能耗均衡效果与CHCS相当,明显优于LEACH。结果显示TBCS-EA综合性能较之于现有算法有明显提升。     

车载式土壤光-电特性参数采集系统研究

土壤电导率是衡量土壤传导电流能力的一种固然属性,除了能够反映土壤质地外,还能够反映土壤的含水率、含盐量、有机物含量等特性。利用土壤光谱数据可以分析出土壤含水率、养分等,且测量过程中无需采样、搅动土壤。通过土壤电导率数据和光谱数据的综合、校正,能够提高系统的精度。基于嵌入式技术开发了土壤电导率和光谱反射率综合检测系统。土壤电导率测量系统采用基于改进的电流-电压四端法原理,使用深松犁的尖端作为电极传感器,能够在测量的同时完成松土的作用。光谱测量系统使用微型光谱传感器采集光谱数据,并进行实时处理。在采集土壤电导率、光谱数据的同时,系统同步采集GPS信息,并和土壤电导率、光谱数据一起保存,供进一步绘制土壤特性分布图使用。系统能够实时综合处理多种数据,并进行显示、保存等操作,具有良好的应用 前景。     

近红外多组分分析中异常样本识别方法

近红外光谱分析中,异常样本的存在严重影响定标模型的预测性能和适配性。基于 X / Y 联合的ODXY异常样本识别和剔除方法,提出并证明了一种专用于多组分分析的MODXY异常样本识别方法。实验采用80组玉米近红外光谱数据,利用不同异常样本识别方法剔除异常样本后建立玉米含水率、含油率、蛋白质含量和淀粉含量4种组分的偏最小二乘预测模型,采用预测均方差和决定系数作为评价指标比较所建模型的性能,检验MODXY方法在多组分分析中的异常样本识别能力。实验结果表明：在近红外多组分分析中,MODXY方法在大多数情况下具有更好的异常样本识别能力;MODXY方法和ODXY方法均有一定的适用范围,它们更适合于相应组分化学值的相对标准偏差较大的情况。     

Validation of tuber blight (Phytophthora infestans) prediction model

Potato tuber blight caused by Phytophthora infestans accounts for significant losses of tubers in storage. Despite research on infection and management of tuber blight, there is paucity of information on the prediction of the occurrence tuber blight or modelling of tuber infection by P. infestans under field conditions. A tuber blight prediction model was developed in New York in experiments conducted using cultivars Allegany, NY101, and Katahdin in 1998 and 1999. This model was validated using data collected from the potato cultivar Snowden in field experiments in Laingsburg, Michigan from 2000 to 2009. In both New York and Michigan experiments, disease was initiated by artificial inoculation of cultivars with a US-8 isolate of P. infestans. Mean leaf area affected ranged from 0 to 94% at New York, and 0 to 93% at Michigan. At New York and Michigan, mean tuber blight incidences ranged from 1 to 40% and 0 to 15%, respectively. In the validation of the model using data collected at Laingsburg, Michigan, the model correctly predicted tuber blight incidence in 7 out of 9 years. Comparison of observed with predicted values indicated that slopes of the regression line between observed and predicted germination and infection data were not significantly different (P > 0.3547). Correlation coefficient between observed and predicted values was high (r² > 0.65) and the coefficient of variation of the residuals of error was about 12%. Although inoculum availability is assumed in the model, incorporation of relationships of inoculum density, propagule survival in soil, and tuber blight incidence would greatly improve the prediction of tuber blight under field conditions.     

Binding of ciprofloxacin labelled with technetium Tc 99m versus 99mTc-pertechnetate to a live and killed equine isolate of Escherichia coli

This paper describes a simple methodology for evaluating the bacterial binding of ciprofloxacin labelled with technetium Tc 99m. Using this methodology, the binding of 99mTc-ciprofloxacin by live Escherichia coli was compared with the binding of 99mTc-ciprofloxacin by killed E. coli and the binding of 99mTc-pertechnetate (99mTcO4-) by live E. coli. The antimicrobial effect of 99mTc-ciprofloxacin on E. coli was evaluated. Four groups were defined: live E. coli with 99mTc-ciprofloxacin, live E. coli with 99mTcO4 , killed E. coli with 99mTc-ciprofloxacin, and killed E. coli with 99mTcO4-. After 0, 2, and 4 h of incubation of 1 x 10(8) colony-forming units of E. coli suspended in 5 mL of sterile distilled water with 1.85 MBq of 99mTc-ciprofloxacin or 99mTcO4, 1 mL from each sample was centrifuged. The radioactivity of the bacterial pellet and that of the supernatant were measured separately, and the percentage of sample radioactivity attributable to bacterial binding was calculated. Of the 99mTc-ciprofloxacin, 3.6% to 5.9% was bound to live or killed E. coli; only 0.1% to 0.2% of the 99mTcO4- was bound to live E. coli (P < 0.0001). No significant difference in 99mTc-ciprofloxacin binding was found between live and killed E. coli (P = 0.887). An antimicrobial effect on E. coli was seen with 99mTc-ciprofloxacin: colony counts were reduced after 4 h. The small amount of 99mTc-ciprofloxacin binding and the lack of difference in binding between live and killed E. coli may limit the utility of this methodology in evaluating the presence of E. coli infection.     

百花花楸活性成分季节变化研究

研究百花花楸活性成分季节变化,以期为百花花楸活性成分药用价值开发提供理论基础。以百花花楸不同季节枝、叶、果为原料,采用化学系统鉴别法及紫外-可见分光光度法对总黄酮、总多糖、总萜进行定量。百花花楸不同部位化学成分有一定区别,主要在皂苷、蒽醌、及甾醇类;随季节总黄酮、总多糖及总萜有一定变化,一般来说7、8、9月为最佳采摘季节。百花花楸活性成分季节变化较为明显,且常见活性成分含量较高,尤其果实和叶,有一定食用及药用价值。     

Comparison of a maedi-visna virus CA-TM fusion protein ELISA with other assays for detecting sheep infected with North American ovine lentivirus strains.

A maedi-visna virus CA-TM fusion protein ELISA (MVV ELISA) was evaluated for the detection of antibody in sheep infected with North American ovine lentivirus (OvLV). The results of the MVV ELISA were compared with other assays for OvLV antibody and with viral infection in an intensively studied group of 38 sheep with a high prevalence of OvLV infection and disease. The sensitivity, specificity, and concordance of assays for OvLV antibody (MVV ELISA, indirect ELISA, Western blot, and AGID), virus (virus isolation, PCR, antigen ELISA), and OvLV-induced disease in each animal were compared with OvLV infection status as defined by a positive result in two or more of the assays. Five sheep met the criteria for absence of OvLV infection. The sensitivity of the MVV ELISA in detecting OvLV infected sheep was 64%, whereas the sensitivity of the other three tests for antibody ranged from 85 to 94%. All the antibody assays were 100% specific in this group of animals. Of the assays for virus, the PCR test had the highest sensitivity and the best concordance with OvLV infection, but it also had the lowest specificity of any of the virus or antibody assays. Among the antibody tests, the concordance of the MVV ELISA compared most favorably with the AGID test for detecting OvLV-infected sheep. Analysis of serum samples from 28 lambs experimentally-infected with one of three North American strains of OvLV suggested that there were no significant strain differences detectable by antibody assay. Twenty virus-inoculated lambs were positive by both the MVV ELISA and the AGID test, five lambs were MVV ELISA negative and AGID test positive, and three lambs were MVV ELISA positive and AGID test negative. No pre-inoculation samples were positive by either assay. In a longitudinal study involving seven lambs, antibodies to OvLV were detected by AGID 3-5 weeks post-inoculation, but were not detected by MVV ELISA until 5-10 weeks post-inoculation. Among 128 naturally and experimentally-infected sheep that were seropositive in the AGID test, the overall sensitivity of the MVV ELISA was higher in the naturally infected sheep (84%) than in the experimentally infected sheep (69%). The data indicated that the MVV ELISA represents a less sensitive, but specific alternative for the detection of OvLV antibodies.     

Clearance of bacteria in turkeys with Bordetella avium-induced tracheitis

Quantitative clearance of aerosolized Escherichia coli from the trachea, lung, and air sacs was measured in turkeys infected with Bordetella avium. Clearance of E. coli in turkeys with B. avium-induced tracheitis was minimally affected early in infection. Sixteen to 23 days after infection with B. avium, sporadic, mild depressions in clearance of E. coli were observed in the tracheas, which had large areas of deciliated tracheal epithelium or replacement of normal epithelium by immature hyperplastic epithelium or metaplastic squamous epithelium. Clearance of E. coli from the lung and air sacs was minimally affected in turkeys infected with B. avium.