指纹图谱技术在云南省烤烟质量分类中的应用研究

选择合适的方法对烟叶质量进行科学合理的分类,对于指导烤烟配方打叶工作及提高卷烟质量具有重要意义.试验采用高效液相色谱指纹图谱技术,结合化学计量学分析方法,建立了云南省15个典型烟叶产区C3F等级烟叶基于指纹图谱特征的烟叶质量分类方法.结果表明,2010年收获的云南省典型烟叶产区的烟叶质量可分成4个基本类型,一类产区包括富源县、沾益县、马龙县和大姚县,二类产区包括宣威市、禄劝县、保山市、丽江市和景东县,三类产区包括麒麟区、罗平县、红河县和文山县,四类产区包括临沧市和昭通市.试验研究结果显示,烟叶指纹图谱作为烟叶质量控制的新方法,可以减少评吸师的工作量,提高配方打叶的工作效率.     

Synchronization of flowering in maize (Zea mays L.) by the genetic system pg11 pg12

Summary The mutant genotype pg11pg11 pg12pg12-epistasis of double recessive factors-determines greater precocity of flowering. The possibility of using this mutant in the production of hybrid seed as a mean of synchronizing flowering in parent strains of different earliness is studied. The differences between mutant and green control genotypes in the characters of days to pollen shedding, pollen production, and height of plants are evaluated. Four mutant lines are considered together with their respective green near-isolines in two distinct localities and across four successive plantings.The mutant near-isolines presented lower values than those of the green near-isolines for all the characters. The maximum reductions in days to flowring were obtained in early plantings and late maturity strains. The reduction in pollen production was about 50%. The reduced height of the mutants is explained, at least in part, by the correlation of this factor with days to flowering. The mutants did not prove to be more sensitive to the environment than their green near-isolines. Seed production was not studied since the mutant is very deficient for this character and it could therefore only be utilized as the male parent. We concluded that the mutant, could be used to synchronize flowering in the industrial production of hybrid seed.     

手持式风送授粉机工作参数优化与试验

针对果树授粉作业普遍存在授粉量大、授粉作业不均匀等现象,采用Box-Benhnken的中心组合试验设计理论对风送式授粉作业工作参数作优化研究。以手持式风送授粉机为研究对象,以喷管直径、收缩管直径、电机电压等工作参数为影响因素,以花粉授粉量、喷管出口固相质量浓度、花粉覆盖率为目标函数,建立两者之间的多元数学回归模型,探索各因素之间的影响规律及最佳水平组合。利用Design-Expert 8.0.6软件的回归分析法和响应面分析法对模型进行优化分析,得到风送式授粉机最优工作参数。性能试验结果表明:喷粉量、固相质量浓度影响因素显著顺序依次为喷管直径、电机电压、收缩管直径;覆盖率影响因素显著顺序依次为电机电压、收缩管直径、喷管直径;最优参数组合为喷管直径为5 mm、收缩管直径为7.5 mm、电机电压为8.0 V,对应的花粉喷粉量、固相质量浓度、覆盖率分别为1.70 g/min、17.28 g/m3、77.71%,且各性能指标与理论优化值相对误差均小于6%。田间试验结果表明:猕猴桃花朵柱头的覆盖率为67.11%,与性能试验结果相比相对误差小于16%。研究结果可为进一步完善风送授粉机的结构设计和作业参数优化提供参考。     

东牙溪流域的水土流失现状分析及治理对策

本文通过对三元区东牙溪流域水土流失现状、分布、类型、危害及其原因调查分析，提出了水土流失治理对策。     

论西南地区的草地生态建设

在阐述西南的地理位置以及草地资源特征的基础上,分析了西南草地建设对于生态环境、畜牧业及农业发 展的重大作用,并提出了进行草地建设的一些措施。     

爪式粉碎机筛片吸声结构模型的建立与分析

本文通过建立爪式粉碎机筛片吸声结构的模型,分析了筛片的吸声降噪原理,以及吸声性能与筛片有关参数的关系,并做了试验验证。为在设计粉碎机时能综合考虑这些因素提供了依据。     

模糊优选模型在流域梯级水电开发中的应用

针对流域梯级水电开发优先决策问题,应用模糊优选模型对指标的相对优属度求解。首先对指标进行模糊化,通过模糊多目标运算得到各目标的相对隶属度,进而确定各个水电站在流域梯级开发中的位置。实例表明,该方法简单、有效,排序结果合理。     

益生菌SFU—9发酵工艺的确定

益生菌具有促进动物生长，提高机体免疫能力等生理功能，常用作微生态饲料添加剂。菌体SFU－9是一株蜡状芽杆菌，该菌株在不利环境下能够以孢子形式存在，具有耐高温，耐酸碱和耐挤压等优点，较乳酸菌，酵母菌等益生菌更能保持活菌状态，适合饲料加工业的需要。该研究以菌株SFU－9进行实验，研究发酵温度，接种量，培养基初始pH值，溶解氧等因素对菌株生长的影响，确定出最佳培养条件为：培养基初始pH值为6．0－6．5，接种量5％（相对摇瓶装液量），培养温度30－32℃，装液量28-33mL/250mL。发醇液中的活菌数可达10^11cfu/mL。在此基础上，采用单因子和正交实验相结合的方法，确定出最佳发酵培养基组成为：玉米淀粉2．0％，啤酒酵母1．5％，NaH2PO40.2%,Na2HPO40.2%,玉米浆2．0％。是以最佳培养基配方给菌体提供营养，在最佳培养条件下培养，可以使发酵液中的活菌数高达10^12cfu/mL.     

The outer membrane protein P2 (OmpP2) of Haemophilus parasuis induces proinflammatory cytokine mRNA expression in porcine alveolar macrophages

Porins, expressed by Gram-negative bacteria, have several biological effects on host tissues or cells. The outer membrane protein P2 (OmpP2), a member of the porin family, has been identified as a multifunctional protein involved in the pathogenicity of Haemophilus parasuis. In the present study, it was shown that OmpP2 (0.5–10 μg/mL) from H. parasuis Nagasaki strain up-regulated mRNA expression of interleukin (IL)-1α, IL-1β, IL-6 and IL-8 in porcine alveolar macrophages (PAM, 3D4/31) in vitro, in a dose-dependent manner. Moreover, OmpP2 porin induced a more prolonged cytokine response in PAM than that of the lipooligosaccharide (LOS) from this microorganism. The data demonstrate that H. parasuis OmpP2 can stimulate proinflammatory cytokine mRNA expression, suggesting that this particular porin might play an important role in the pathogenesis of disease caused by H. parasuis.