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101.
Elienai Ferreira da Silva Mara Regina Moitinho Daniel De Bortoli Teixeira Elton da Silva Bicalho Guilherme Adalberto Ferreira Castioni Gener Tadeu Pereira Newton La Scala 《Soil Use and Management》2020,36(4):658-670
Soil CO2 emission (FCO2) in agricultural areas results from the interaction of different factors such as climate and soil conditions. Our objective was to investigate the spatiotemporal variation of FCO2, temperature (Tsoil), moisture (Msoil) and air-filled pore space (AFPS), as well as their interactions, during the sugarcane field reform. The study was conducted on a 90 × 90 m sampling grid with 100 points at 10 m spacings. Ten assessments of FCO2, Tsoil and Msoil were carried out at each point over a 28-day period. The greatest mean values of FCO2 (0.74 g m−2 hr−1) and Msoil (31.7%) were obtained on Julian day 276, 2013, being associated with precipitation events at the study site. Also, the smallest values of AFPS (19.17%) and Tsoil (20.90°C) were observed on the same day. The spatial variability of FCO2, Tsoil, Msoil and AFPS was best described by an adjusted spherical model, although an exponential model better fitted some results. The spatial pattern of all soil attributes showed little temporal persistency, indicating a high complexity for FCO2 during precipitation. Correlation maps assisted in identifying regions where Msoil and AFPS better controlled the emission process and where Tsoil was important. A major challenge for world agriculture is to increase the efficiency of conventional soil management practices. We highlight the importance of the spatial pattern of soil properties that directly influence the CO2 emission dynamics. Future mitigation actions should involve less intense tillage and ensure homogeneous applications of soil inputs, thereby reducing production costs and the contribution of these activities to CO2 emissions during the sugarcane field reform. 相似文献
102.
Alessio Dileta Regina Moro Velho João Pedro Tambara Antônio Augusto Cortiana de Oliveira Gomes Ivan Pedro Knob Deise Aline Haygert-Velho Ione Maria Pereira Busanello Marcos Thaler Neto André 《Tropical animal health and production》2020,52(1):185-193
Tropical Animal Health and Production - To reduce costs, lactating cows are often fed a higher proportion of roughage and alternative feeds; however, such protocols may not lead to optimal milk... 相似文献
103.
Santos OC Barros EM Brito MA Bastos Mdo C Dos Santos KR Giambiagi-Demarval M 《Veterinary microbiology》2008,130(1-2):134-140
Coagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. These consequences impose serious economic losses for the farmers and the dairy industry. Routine veterinary laboratories do not usually identify CNS at the species level. Thereby, the aims of this study were to identify the most common staphylococcal pathogens involved in bovine mastitis using PCR-restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and to compare our results with the identification carried out by the conventional method. A total of 54 isolates of Staphylococcus, involved in bovine mastitis, were analyzed by this method. The size and number of the fragments obtained by either AluI or HindIII/PvuII digestions made possible to form clear patterns differentiating, among the isolates, 11 of the most common species of animal staphylococcal pathogens. Most of the isolates clustered together with the reference strain of Staphylococcus chromogenes (28) and the type strain of Staphylococcus epidermidis (8). Besides, some isolates clustered together with the type strain of Staphylococcus aureus (5). All patterns were confirmed by the conventional biochemical method, showing concordant results. Thus, the PCR-RFLP of the groEL gene constitutes a reliable and reproducible molecular method for identification of CNS species responsible for bovine mastitis. 相似文献
104.
Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas 总被引:1,自引:0,他引:1
Wengi N Willi B Boretti FS Cattori V Riond B Meli ML Reusch CE Lutz H Hofmann-Lehmann R 《Veterinary microbiology》2008,126(1-3):132-141
Hemotropic mycoplasmas (hemoplasmas) have been reported in several mammalian species including dogs. Infections may lead to hemolytic anemia, but investigations in the dog had been hampered by the lack of adequate diagnostic methods. Only recently sensitive PCR-based assays were reported for the two canine hemoplasmas, Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum. By applying these assays, 15.4% of 460 dogs from the south of France tested hemoplasma positive. It was hypothesized that this high prevalence may be associated with the presence of Rhipicephalus sanguineus, a proposed vector for canine hemoplasmas. To address this hypothesis and expand the PCR-based knowledge on canine hemoplasmosis, we investigated dogs in a climatic zone that does not allow for the permanent establishment of R. sanguineus. Blood samples were collected throughout a year from 889 dogs in Switzerland: 1.2% of the dogs tested real-time PCR positive. The infection status was not significantly associated with anemia, age or gender. Phylogenetic analyses of Candidatus M. haematoparvum and M. haemocanis isolates revealed > or =99.8% identity to published sequences. All samples collected from three infected dogs throughout a follow-up period of < or =13 months tested PCR positive. Interestingly, the majority of the infected dogs either had been imported from or had visited regions where R. sanguineus is indigenous. Thus, canine hemoplasma prevalence was found to be low in a country with a climate incompatible with frequent occurrence of R. sanguineus. Nonetheless, veterinarians may expect hemoplasma infections in dogs with a travel history and/or after potential tick vector exposure. 相似文献
105.
Computed tomographic features of incomplete ossification of the canine humeral condyle 总被引:1,自引:0,他引:1
Objectives— To describe computed tomographic (CT) features of canine elbows with incomplete ossification of the humeral condyle (IOHC) and investigate co-existing incongruence in the elbow joint.
Study Design— Case control study.
Animals— Dogs with IOHC (n=20; 38 elbows) and 25 normal elbows.
Methods— Elbows with IOHC and normal elbows were assessed by CT. Standardized dorsal and sagittal reconstructions were created at 3 levels using image analysis software to obtain single measurements of the humero-radial and humero-ulnar joint spaces. On dorsal plane reconstructions, joint space measurements were obtained at the center point of the humero-radial and humero-ulnar articulations. Joint incongruity was defined as the difference between the humero-radial and the humero-ulnar joint spaces.
Results— Nineteen dogs (95%), all Spaniel breeds, had either bilateral IOHC demonstrable as a saw-toothed intercondylar complete or incomplete hypoattenuating defect with hyperattenuating margins, or IOHC with contralateral humeral condylar fracture (HCF). Joint incongruity values for IOHC were compared with those of normal elbows. Significant differences were noted at the levels of the medial coronoid apex ( P <.0001) and base ( P <.004) indicative of humero-ulnar incongruence. Evidence of medial coronoid disease in 10 elbows (26%) and degenerative joint disease in 30 elbows (79%) was also found.
Conclusions— Presence of elbow incongruence may be an underlying factor in failure of ossification centers to fuse leading to IOHC.
Clinical Relevance— IOHC is clearly defined by CT, and it should be considered in larger Spaniel breeds, with a chronic forelimb lameness or HCF. 相似文献
Study Design— Case control study.
Animals— Dogs with IOHC (n=20; 38 elbows) and 25 normal elbows.
Methods— Elbows with IOHC and normal elbows were assessed by CT. Standardized dorsal and sagittal reconstructions were created at 3 levels using image analysis software to obtain single measurements of the humero-radial and humero-ulnar joint spaces. On dorsal plane reconstructions, joint space measurements were obtained at the center point of the humero-radial and humero-ulnar articulations. Joint incongruity was defined as the difference between the humero-radial and the humero-ulnar joint spaces.
Results— Nineteen dogs (95%), all Spaniel breeds, had either bilateral IOHC demonstrable as a saw-toothed intercondylar complete or incomplete hypoattenuating defect with hyperattenuating margins, or IOHC with contralateral humeral condylar fracture (HCF). Joint incongruity values for IOHC were compared with those of normal elbows. Significant differences were noted at the levels of the medial coronoid apex ( P <.0001) and base ( P <.004) indicative of humero-ulnar incongruence. Evidence of medial coronoid disease in 10 elbows (26%) and degenerative joint disease in 30 elbows (79%) was also found.
Conclusions— Presence of elbow incongruence may be an underlying factor in failure of ossification centers to fuse leading to IOHC.
Clinical Relevance— IOHC is clearly defined by CT, and it should be considered in larger Spaniel breeds, with a chronic forelimb lameness or HCF. 相似文献
106.
107.
Luan C. Henker Regina T. Kemper Simone P. Bianchi Marcele B. Bandinelli Saulo P. Pavarini 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(2):255-258
A 9‐year‐old mixed breed 13 kg spayed female dog was presented for evaluation of two masses in the right abdominal mammary gland region. Surgery was conducted to excise the masses. A grade I complex mammary gland carcinoma and high grade (grade III) mast cell tumor with an inguinal lymph node metastasis were diagnosed. Forty‐seven days after the surgical procedure, the mast cell tumor relapsed, and neoadjuvant treatment with lomustine (81 mg/m2) was prescribed. Thirteen days from initiation of lomustine therapy, the dog was re‐presented to the hospital with bloody diarrhea, hematemesis, epistaxis, an elevated rectal temperature, depression, severe dehydration, and marked dyspnea. The CBC showed severe thrombocytopenia and leukopenia. According to the owner, lomustine (45mg per os [PO]) was mistakenly administered daily for 10 consecutive days (total dose, 810 mg/m2). The dog died and a necropsy was performed. The main gross lesions consisted of severe multifocal hemorrhages in multiple organs, especially in the digestive system. Histopathologic evaluation revealed disseminated hemorrhages, as well as marked bone marrow aplasia. This report describes the clinical, hematologic, gross, and histologic findings in a fatal case of lomustine overdose in a dog. 相似文献
108.
Balaro Mario Felipe A. de Mello Samuel Guaraná Valverde da Silva Santos Alex Cavalcanti Luiza Mattos Almosny Nádia Regina Pereira Fonseca Jeferson F. Brandão Felipe Z. 《Tropical animal health and production》2019,51(2):345-353
Tropical Animal Health and Production - This study aimed to verify the reproductive seasonality in Saanen goats from distinct parity orders (nulliparous, primiparous, and pluriparous) throughout an... 相似文献
109.
Regina Campbell-Malone Susan G Barco Pierre-Yves Daoust Amy R Knowlton William A McLellan David S Rotstein Michael J Moore 《Journal of zoo and wildlife medicine》2008,39(1):37-55
Vessel-whale collision events represented the ultimate cause of death for 21 (52.5%) of the 40 North Atlantic right whales (Eubalaena glacialis) necropsied between 1970 and December 2006. Injuries seen in vessel-struck whales fall into two distinct categories: 1) sharp trauma, often resulting from contact with the propeller, and 2) blunt trauma, presumably resulting from contact with a vessel's hull. This study analyzes four trauma cases that resulted from vessel-whale collisions, which together provide a framework for a more critical understanding of lethal blunt and sharp trauma resulting from vessel collisions with right whales. In case no. 1, contact with a propeller resulted in three deep lacerations. The animal survived acute trauma only to succumb nearly 14 years later when the lesions reopened and became infected. In case no. 2, anecdotal reports linked the laceration of large arteries of the peduncle and histologic evidence of perimortem trauma at a bone fracture site to vessel-whale collision trauma. Case no. 3 had a laceration of the oral rete and a fracture of the rostrum. Both of the areas displayed histologic evidence of perimortem blunt trauma. Finally, in case no. 4, an antemortem mandibular fracture, two additional skull fractures, and widespread hemorrhage were consistent with severe blunt trauma. Evidence from each case, including the timing of trauma relative to the time of death and identifying characteristics of both trauma types, are presented. Before this study, no detailed comparative analysis of trauma pathology that resulted from lethal interactions between vessels and right whales had been conducted. This study demonstrates the importance of detailed gross and histologic examination in determining the significance and timing of traumatic events. This work represents a new paradigm for the differential diagnosis of lethal sharp and blunt trauma in right whales hit by ships and will enhance the present understanding of the impact of anthropogenic mortality on this critically endangered species. 相似文献
110.
K Christiana Grim Thomas McCutchan Margery Sullivan Michael R Cranfield 《Journal of zoo and wildlife medicine》2008,39(2):216-220
A pair of Australian black swans (Cygnus atratus) with origins in Wakefield, Virginia, USA, was admitted to the quarantine area at the Baltimore Zoo for general health assessments before housing in the collections. During the quarantine period, no clinical signs of disease were manifest; however, upon examination of a blood smear, intraerythrocytic parasites were detected and initially determined to be Haemoproteus species. Diagnostic polymerase chain reaction (PCR) and sequencing results, however, indicated that the parasites were within the genus Plasmodium. Subclinical infections with Plasmodium species in birds may affect collection management, and transmission from refractory hosts to susceptible hosts should be considered when multispecies exhibits are used. In addition, changes in the dynamics of host-vector-parasite interactions might have significant impacts on wild or domesticated populations of birds. 相似文献