滑县花生新黑地蛛蚧的发生与防治技术

花生新黑地蛛蚧是近年来滑县花生田新发现的一种地下害虫,对花生造成危害,影响花生的 品质和产量。本文主要介绍新黑地蛛蚧的形态特征、发生规律、生活习性、为害症状及防治措施。     

Potential of Plant Proteins Digested In Silico by Gastrointestinal Enzymes as Nutritional Supplement for COVID-19 Patients

Plant Foods for Human Nutrition - Currently, no specific drug and vaccine are available for the new coronavirus SARS-CoV-2, and nutritional supplementation should be helpful. This study tried to...     

芒果中性/碱性转化酶MiNI基因的克隆与表达分析

采用RT-PCR技术克隆了1个编码NI基因的cDNA序列,命名为MiNI基因,并对不同来源的中性/碱性转化酶的分子特征及系统进化进行比较分析。结果表明：MiNI基因开放阅读框为2034 bp,编码677个氨基酸,相对分子量为76.6 kDa,理论等电点为6.24;生物信息学分析结果显示,NI二级结构α螺旋占38.85%,无规则卷曲占35.45%,伸展链占18.91%,β折叠占6.79%;MiNI具有glycoside hydrolase family 100结构保守域,与克里曼丁桔、龙眼、巴西橡胶树和番木瓜都具有一致的motif位点;MiNI基因编码的氨基酸序列与克里曼丁桔、龙眼氨基酸序列同源性最高;构建NI系统进化树分析表明,与芒果遗传距离最近的是克里曼丁桔,最远的是玉米和枸杞。qRT-PCR分析显示,果皮MiNI基因表达量远高于果肉;花后10~40 d,果皮MiNI基因表达量显著下降;花后40~100 d,果皮MiNI基因表达量维持在一个相对稳定水平;花后100~130 d,随着果实成熟,果皮MiNI基因表达量又显著上升;而花后10~40 d,果肉MiNI基因表达量显著下降,至果实发育后期果肉MiNI基因表达量始终处于极低水平。该研究为进一步了解MiNI基因在芒果果实蔗糖代谢过程中的作用以及从分子角度阐明芒果糖代谢机理奠定理论和技术基础。     

基于CiteSpace可视化分析的茶叶香气研究进展

以1979—2019年WOS(Web of science)和CNKI(China national knowledge infrastructure)收录的茶叶香气品质相关文献为研究对象,采用CiteSpace文献计量方法分别从年代、作者、机构、国家、研究热点、演进趋势等方面进行归纳统计分析。结果表明,2006年以后相关研究文献呈显著增长趋势,目前已形成稳定的核心作者群,但各群体间的合作研究相对较少;在该研究领域,我国影响力最大,其次是日本和美国;热点研究内容主要集中在香气形成机理、香气物质提取方法、检测手段及关键香气物质等方面。综上结果,结合时区图谱,进一步指出茶叶香气的研究历程及目前所处的发展阶段。     

人参/西洋参枯叶病缓解实验

通过各供试药剂在西洋参枯叶病上的使用,验证各供试药剂对西洋参枯叶病的缓解作用。结果表明,参多乐450倍液+益微450倍液对西洋参枯叶病缓解效果突出,在一定程度上解决了枯叶病给西洋参带来的危害。且该配方在人参上同样适用。     

Analysis of the water balance and the nitrogen and phosphorus runoff pollution of a paddy field in situ in the Taihu Lake basin

Paddy and Water Environment - Nitrogen (N) and phosphorus (P) runoff loads of paddy fields in situ under conventional water and fertilizer management measures caused severe water pollution in the...     

葡萄新品种妮娜女皇的引种表现和优质栽培技术

2014年引入妮娜女皇葡萄一年生苗木在淮安市园艺技术服务站果树示范基地试栽,经过5年试验观察,表现优良,经赤霉素和氯吡苯脲处理后,平均穗重680g,平均单果重17g,可溶性固形物含量平均18%,最高可达21%,甘甜可口,苗木栽植第4年每666.7m^2产量1000~1250kg。适应性强,丰产性良好,可推广。总结了妮娜女皇葡萄的优质栽培技术。     

白桦新品种‘朝霞2号’

‘朝霞2号’是以白桦为受体获得的转BpGLK1基因干扰表达株系,其叶片中BpGLK1的表达量降低,叶绿素含量减少。主干树皮灰白色,枝条红褐色。叶片纸质,宽卵形,夏季呈黄绿色,秋季呈金黄色,叶尖呈渐尖,叶基心形,叶缘钝齿、无叶裂,上下表面均有绒毛分布,一级侧脉7 ~ 8个。     

Occurrence of prophage and historical perspectives associated with the dissemination of huanglongbing in mainland China

Huanglongbing (HLB), associated with a non-culturable bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), is a highly destructive citrus disease with a long but poorly documented history in China. No effective treatment for HLB is available. The identification of new prophages in abundant CLas genomic sequence data provides new insights into both the diversity of CLas strains and HLB management. In this study, CLas populations from nine provinces were surveyed for the presence of prophage. Two major prophage typing groups (PTGs) were discovered to be associated with two different altitude regions: strains of CLas in PTG1 from high altitude regions (HAR) mainly contained prophage Type 1 only or Types 1 and 3, whereas strains of CLas in PTG2 from low altitude regions (LAR) mainly contained prophage Type 2. The discovery of these CLas population patterns provides evidence for independent origins of HLB in HAR and LAR. Guangdong province is the generally recognized domestic region of origin for HLB and is primarily responsible for the dissemination of HLB in LAR through transport of seedlings. Both Yunnan and Sichuan provinces are the probable regions of origin for HLB in HAR. PTG2 was further divided into two subgroups: PTG2-1, found in Guangdong, Fujian and Guangxi and PTG2-2, found in Jiangxi, Zhejiang and Hunan. These regions and prophage types are correlated with early and late introductions of HLB in LAR. These molecular analyses were supported by studying the history of the dissemination of HLB in historical documents.