Baicalin suppresses expression of Chlamydia protease-like activity factor in Hep-2 cells infected by Chlamydia trachomatis

In this study, we tested the ability of Baicalin to block Chlamydia trachomatis infection and found that the Baicalin blocked infection of Hep-2 cells. Then, we looked into the expression of RFX5 and CPAF gene in Chlamydia-infected cells. We found that RFX5 and CPAF were up-regulated and down-regulated respectively by Baicalin. Since CPAF is responsible for degrading RFX5, we suggest that CPAF was a primary target of Baicalin and played an important role in regulating RFX5. Our findings demonstrate that Baicalin can inhibit C. trachomatis effectively and therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.     

蚜灰蝶及其他3种捕食性天敌对竹蚜的控制作用

在我国,竹类是一类广为栽植的经济林和用材林树种,同时也发展成为城市园林重要的观赏植物,而竹蚜是竹林的一类重要害虫,常造成竹林生长衰退,进而引发的竹林霉污病可进一步加剧竹林残败,并降低城市园林竹类的观赏价值。据安徽皖东竹林     

日本金竹文化论

日本的金竹文化鲜明体现在古代战争、传统节日、文学等方面,具有历史悠久、内涵深刻、表现广泛等特点。近代以后金竹文化低迷,但仍然是日本竹文化中最具神圣文化内涵的部分。     

Molecular identification of a phytoplasma associated with Sophora Root yellows

A phytoplasma infecting Sophora Root (Sophora alopecuroides) was detected and identified in Alar, Xinjiang Uygur Autonomous Region of China. Typical phytoplasma bodies were observed in sieve tubes of the diseased plants by transmission electron microscopy. A partial 16S rRNA gene and ribosomal protein (rp) genes containing rpl22 (rplV) and rps3 (rpsC) were amplified by direct and nested PCR. Based on the sequence similarity of the 16S rRNA and rp genes with accompanying phylogenetic analyses, the phytoplasma associated with Sophora Root yellows belongs to the 16SrI group (aster yellows group). Virtual RFLP analysis of these 16S rRNA and rp gene sequences showed distinct differences from those of reference phytoplasma strains representing previously described subgroups of the 16SrI group. Moreover, the similarity coefficient (0.92) of the RFLP profile of this phytoplasma was less than the threshold similarity coefficient (0.97) required for subgroup classification. Thus, the phytoplasma isolate of Sophora Root plants, designated as ‘SoRY’, represents a new subgroup. Furthermore, this is the first report of phytoplasma disease associated with Sophora Root plants.     

EOS/MODIS遥感监测在甘肃迭部重大森林火灾中的应用

森林生态系统是陆地自然生态系统中最大的生态系统,它在整个自然界的动态平衡中具有重要的作用.森林火灾是生态环境的一种重大自然灾害,全世界每年发生森林火灾几十万次.特别是20世纪80年代以来,全球气候持续变暖,林火有上升的趋势(舒立福等,1997).     

中国综合花卉市场营销策略研究

花卉市场作为花卉产品的集散中心,给花卉业的建设和发展带来了新的生机和活力,大大促进了花卉产业化的形成和发展。文章采用典型调查、动态研究、比较研究的方法,研究分析了我国花卉市场营销的发展现状、存在的问题,并提出了中国现阶段综合性花卉市场的定位、选址、规模、管理模式、经营特色、市场行为规范、售后服务、宣传方式、打造特色品牌、设施建设等方面的营销策略,以期望能够对中国综合性花卉市场建设具有指导意义。     

Effects of chlorantraniliprole on development and reproduction of beet armyworm, <Emphasis Type="Italic">Spodoptera exigua</Emphasis> (Hübner)

The lethal and sublethal effects of chlorantraniliprole against Spodoptera exigua (Hübner) were evaluated under laboratory conditions by oral exposure of neonate larvae to the compound. The 72 h LC₅₀ value of this insecticide to S. exigua was found to be 12.747 μg l⁻¹. A progressive larval mortality of 24.32% for LC₃₀ treatment and 42.61% for LC₅₀ treatment was observed from 4th to 6th day after exposure, which resulted in the reduced pupation rates in exposure groups. The sublethal effects of this chemical were indicated by prolongation of larval period, the increase of pupal weight and decrease in hatch rate of egg. Chlorantraniliprole at LC₃₀ and LC₅₀ rate significantly delayed larval development; the developmental duration of surviving larvae was extended for 22.5 and 28.6%, respectively, compared with that of control group. LC₃₀ treatment increased the mean weight of pupa and induced to the production of heavier pupa (>150 mg). In LC₅₀ treatment, heavier pupa also showed up but the mean weight of pupa was not influenced. The egg hatch rate in LC₅₀ group was significant lower than that in control and LC₃₀ groups. No significant differences in pupal duration, emergence rate, sex ratio, egg number per female, and longevity of adults were observed among treatments. Chlorantraniliprole had exceptional activity against S. exigua according to concentration–response bioassay in laboratory, and the toxicities were primarily resulted from immediate lethality.     

Mercury distribution and speciation in floodplain soils and uptake into native earthworms (Diplocardia spp.)

Historically as part of its national security mission, the U.S. Department of Energy's Y-12 National Security Facility in Oak Ridge, TN acquired a significant fraction of the world's supply of elemental mercury. During the 1950s and 1960s, a large amount of elemental mercury escaped confinement and is still present in the watershed surrounding the Y-12 facility. Earthworms are key components in natural food chains, providing a food source for many small mammals and important food sources for small birds. The objectives of this study were to investigate the current status of mercury distribution and speciation and determination of mercury bioavailability to native earthworms in floodplain soils of East Fork Poplar Creek (EFPC) after decades of U.S. Department of Energy's remediation. The present study clearly shows that the total mercury in a tested floodplain field of EFPC was significantly below the US Department of Energy target 400 mg Hg/kg. The major mercury form in the current floodplain soils of EFPC is mainly the non-cinnabar mercury bound form in soil silicates (4 M HNO₃-extractable residual fraction). The results show strong linear relationships between mercury concentrations in native earthworms (both mature and immature groups) and the non-cinnabar mercury form. Native earthworms may be used as a potential mercury ecological bio-indicator (bio-marker) for demonstrating mercury bioavailability and ecotoxicity in the ecosystem.     

Rapid Determination of Mercury in Contaminated Soil and Plant Samples Using Portable Mercury Direct Analyzer without Sample Preparation, a Comparative Study

The objective of this study was to determine the efficiency of a portable total mercury analyzer (OhioLumex RA-915+) in comparison with traditional analytical methods, such as inductively coupled plasma atomic emission spectrometry and cold vapor atomic absorption. The quick mercury analytical procedure with the direct mercury analyzer without sample pretreatment (such as sample digestion) was optimized for a variety of environmental samples, including contaminated soil and plant samples. The efficiency was evaluated using practical parameters, such as time required for analysis, sample amount, mercury species, accuracy, and precision/reproducibility, as well as using statistical analysis. Our results demonstrate that these three instrumental methods yielded similar mercury concentration values and statistical data, while the mercury direct analyzer had the advantages of not requiring for sample digestion and only requiring a small quantity of samples for distribution of mercury in a single root, a single root hair, and sub-regions of a single leaf of plants. These factors are used to justify use of the portable direct mercury analyzer under field conditions and validation of the results.     

十字花科黑腐病菌中转录调控因子HpaR1调控一个纤维素酶基因的表达

十字花科黑腐病菌（Xcc8004）中的一个转录调控因子XC2736（npaRI）在致病过程中具有重要的作用。前期研究发现该转录调控因子可能调控胞外纤维素酶的合成。为了解HpaRI对纤维素酶的转录调控机理,本研究对HpaR1进行原核表达纯化,并与488bp的包含XC0639的启动子区DNA片段进行凝胶电泳迁移率试验,发现HpaR1与XC0639启动子可以发生结合。将488bp的XC0639的启动子DNA片段与报告基因gus融合,构建XC0639的报告质粒pGUS0639r,分别导入野生型8004菌株和缺失突变体DM2736中,分析发现在突变体背景下GUS的表达水平比野生型背景明显降低。表明HpaRl正调控XC0639的表达。构建XC0639的极性整合突变体PK0639,检测发现PK0639几乎丧失胞外纤维素酶的活力;通过功能反式互补构建的互补菌株CPK0639可以恢复纤维素酶活性。研究结果表明HpaRl通过调控纤维素酶基因XC-0639的表达来调控细胞的纤维素酶活性。本研究为更深入地了解HpaR1如何调控细菌生理生化功能奠定了基础。