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121.
Microorganisms capable of transforming the pesticide 1-naphthyl N-methyl-carbamate (Sevin) were isolated from soil. Three isolates were able to accelerate the hydrolysis of Sevin to 1-naphthol. Several unidentified intermediates were separated by thin-layer chromatography and also by following the decomposition of Sevin-methyl-14C. Since 1-naphthol is a biological as well as a chemical decomposition product of Sevin, its transformation by the isolated microbes was also studied. A fungus, identified as Fusarium solani, altered 1-naphthol rapidly. Whereas one strain of bacterium degraded the hydrolysis product gradually, another strain accumulated it under certain conditions. Mixed cultures of the investigated microbes were more effective in transforming Sevin than pure cultures, and this phenomenon was also observed with 1-naphthol as substrate with the exception of one bacterial strain. 相似文献
122.
Maxime Duvivier Géraldine Dedeurwaerder Michel De Proft Jean-Marc Moreau Anne Legrève 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(2):325-341
Two kinds of propagules play a role in Mycosphaerella graminicola dissemination: splash-dispersed pycnidiospores and airborne sexual ascospores. A method based on real-time polymerase chain reaction (PCR) assay and using Burkard spore traps was developed to quantify M. graminicola airborne inoculum. The method was tested for its reliability and applied in a spore trap network over a 2-year period in order to investigate the spatio-temporal distribution of airborne inoculum in Belgium. At four experimental sites, airborne inoculum was detected in both years. A seasonal distribution was observed, with the highest mean daily quantities (up to 351.0 cDNA) trapped in July and with clusters detected from September to April. The first year of trapping, a mean daily quantity of 15.7 cDNA of M. graminicola airborne inoculum was also detected in the air above a building in a city where the spatio-temporal distribution showed a similar pattern to that in the field. Mean daily quantities of up to 60.7 cDNA of airborne inoculum were measured during the cereal stem elongation and flowering stages, suggesting that it contributes to the infection of upper leaves later in the season. Most detection, however, tended to occur between flowering and harvest, suggesting significant production of pseudothecia during that period. Variations in mean daily quantities from 1.0 to 48.2 cDNA were observed between sites and between years in the patterns of airborne inoculum. After stem elongation, the quantities detected at a site were positively correlated with the disease pressure in the field. Quantities trapped at beginning of the growing season were also well correlated with the disease level the previous year. Multiple regressions revealed that some factors partly explain the daily variations of airborne inoculum. 相似文献
123.
Janke C Rogowski K Wloga D Regnard C Kajava AV Strub JM Temurak N van Dijk J Boucher D van Dorsselaer A Suryavanshi S Gaertig J Eddé B 《Science (New York, N.Y.)》2005,308(5729):1758-1762
Polyglutamylation of tubulin has been implicated in several functions of microtubules, but the identification of the responsible enzyme(s) has been challenging. We found that the neuronal tubulin polyglutamylase is a protein complex containing a tubulin tyrosine ligase-like (TTLL) protein, TTLL1. TTLL1 is a member of a large family of proteins with a TTL homology domain, whose members could catalyze ligations of diverse amino acids to tubulins or other substrates. In the model protist Tetrahymena thermophila, two conserved types of polyglutamylases were characterized that differ in substrate preference and subcellular localization. 相似文献
124.
Andries K Verhasselt P Guillemont J Göhlmann HW Neefs JM Winkler H Van Gestel J Timmerman P Zhu M Lee E Williams P de Chaffoy D Huitric E Hoffner S Cambau E Truffot-Pernot C Lounis N Jarlier V 《Science (New York, N.Y.)》2005,307(5707):223-227
The incidence of tuberculosis has been increasing substantially on a worldwide basis over the past decade, but no tuberculosis-specific drugs have been discovered in 40 years. We identified a diarylquinoline, R207910, that potently inhibits both drug-sensitive and drug-resistant Mycobacterium tuberculosis in vitro (minimum inhibitory concentration 0.06 mug/ml). In mice, R207910 exceeded the bactericidal activities of isoniazid and rifampin by at least 1 log unit. Substitution of drugs included in the World Health Organization's first-line tuberculosis treatment regimen (rifampin, isoniazid, and pyrazinamide) with R207910 accelerated bactericidal activity, leading to complete culture conversion after 2 months of treatment in some combinations. A single dose of R207910 inhibited mycobacterial growth for 1 week. Plasma levels associated with efficacy in mice were well tolerated in healthy human volunteers. Mutants selected in vitro suggest that the drug targets the proton pump of adenosine triphosphate (ATP) synthase. 相似文献
125.
Fluctuations of landings and environmental conditions in the north-western Mediterranean Sea 总被引:12,自引:1,他引:12
Josep Lloret Jordi Lleonart Ignasi Solé & Jean-Marc Fromentin 《Fisheries Oceanography》2001,10(1):33-50
Most of the monthly catches and CPUE of 13 studied commercial species in the coastal waters of the north-western Mediterranean were significantly positively correlated with run-off of local rivers (Rhône and Muga) and the wind mixing index during the spawning season, with time lags of less than a year (transfer function analyses). Rhône and Ebre interannual fluctuations in run-off were synchronous and were related to the North Atlantic Oscillation (NAO), river run-offs being negatively correlated to high NAO episodes. The fluctuations of river discharges and the wind mixing index were cyclic but not related. The results showed that enhanced hydroclimatic conditions in the NW Mediterranean were favourable for the productivity of the fish and invertebrate stocks, and suggest the presence of linkage between recruitment of Mediterranean species and local (river discharges, wind conditions) and global (NAO) environmental conditions. 相似文献