An intensive multi-site pilot study investigating atmospheric mercury in Broward County,Florida

An intensive multi-site pilot study of atmospheric Hg was conducted in Broward County, Florida in August and September of 1993. Broward County, which contains the city of Fort Lauderdale, is located in southeastern Florida. The county borders the Florida Everglades on the west and the Atlantic Ocean on the east. A network of four sampling sites was set up for 20 days throughout Broward County to measure Hg in both the vapor phase and the particle phase as well as Hg in precipitation. The mean concentrations of total vapor phase Hg measured at two inland sites were found to be significantly higher (3.3 and 2.8 ng/m³) than that measured at a site located on the Atlantic shore (1.8 ng/m³). The mean concentrations of particle phase Hg collected at the two inland sites (51 and 49 pg/m³) were found to be 50% greater than that measured at the coastal site (34 pg/m³). In addition, event precipitation samples were collected at four sampling sites over the 20 day study period and were analyzed for both reactive and total Hg. The mean concentration of total Hg in the precipitation samples was found to be 44 ng/L, with a range of 14 to 130 ng/L. It was determined that further meteorological analysis and a more complete characterization of the aerosol and precipitation composition are needed to identify the probable source(s) contributing to the increased deposition of Hg.     

Denitrification potential in a grassland subsoil: effect of carbon substrates

We examined the potential of a subsoil to denitrify nitrate under optimal anaerobic conditions in a laboratory-based incubation when supplied with a range of C substrates of increasing recalcitrance. Both topsoil and its associated subsoil were supplied with nitrate and either glucose, starch or cellulose. Microbial respiration and the evolution of N₂O and N₂ were measured. The subsoil supported low amounts of microbial activity and responded only to the glucose treatment; with less than one-fifth of the N₂O production measured in the top soil. Overall, our findings demonstrated that the denitrification potential of this particular subsoil is relatively low and that only simple carbohydrates could be utilised readily by the resident microorganisms.     

Development and use of microsatellite markers for genetic diversity analysis of cañahua (<Emphasis Type="Italic">Chenopodium pallidicaule</Emphasis> Aellen)

Cañahua (Chenopodium pallidicaule Aellen) is a poorly studied, annual subsistence crop of the high Andes of South America. Its nutritional value (high in protein and mineral content) and ability to thrive in harsh climates make it an important regional food crop throughout the Andean region. The objectives of this study were to develop genetic markers and to quantify genetic diversity within cañahua. A set of 43 wild and cultivated cañahua genotypes and two related species (Chenopodium quinoa Willd. and Chenopodium petiolare Kunth) were evaluated for polymorphism using 192 microsatellite markers derived from random genomic cañahua sequences produced by 454 pyrosequencing of cañahua genomic DNA. Another 424 microsatellite markers from C. quinoa were also evaluated for cross-species amplification and polymorphism in cañahua. A total of 34 polymorphic microsatellite marker loci were identified which detected a total of 154 alleles with an average of 4.5 alleles per marker locus and an average heterozygosity value of 0.49. A cluster analysis, based on Nei genetic distance, clearly separated from wild cañahua genotypes from the cultivated genotypes. Within the cultivated genotypes, subclades were partitioned by AMOVA analysis into six model-based clusters, including a subclade consisting sole of erect morphotypes. The isolation by distance test displayed no significant correlation between geographic collection origin and genotypic data, suggesting that cañahua populations have moved extensively, presumably via ancient food exchange strategies among native peoples of the Andean region. The molecular markers reported here are a significant resource for ongoing efforts to characterize the extensive Bolivian and Peruvian cañahua germplasm banks, including the development of core germplasm collections needed to support emerging breeding programs.     

Identifying indicators of atmospheric nitrogen deposition impacts in acid grasslands

Atmospheric deposition of nitrogen has become a serious concern for nature conservation managers and policy makers. It has the potential to reduce species richness, increase the graminoid component of the sward, encourage species typical of more fertile conditions and alter the soil biogeochemistry of grasslands. Calcifugous grasslands (grasslands found on acid soils) are among the most sensitive to N deposition due to their poorly buffered soils and species typical of nutrient poor environments.Indicators have an important role to play in detecting the impact of nitrogen deposition on sites of conservation importance and assessing conservation status. This study investigates potential indicators of nitrogen deposition impacts that could be incorporated into site condition monitoring programmes such as the UK Common Standards Monitoring.Using two national surveys of calcifugous grasslands we examined the potential for using: the presence or absence of indicator species, the cover of indicator species, the species richness and richness of functional groups, and the cover of functional groups as indicators of N deposition impacts. Of all the potential indicators investigated, graminoid:forb ratio was found to be the best indicator of N deposition impacts. It showed a significant relationship to N deposition in both data sets and is quick and easy to assess in the field. Vegetation indicators must be used with caution as there is potential for vegetation management regime and nutrients from other sources to cause similar changes in species composition. Consideration must be given to these before attributing changes to nitrogen deposition.     

Distribution of grapevine roots and salt under drip and full-ground cover microjet irrigation systems

We describe the three dimensional variation in root length density (L_v) within a quarter of the planting area of Colombard grapevines on Ramsey rootstock grown under drip and full-cover microjet irrigation. Under drip irrigation roots were concentrated under the vine row, whereas under microjet irrigation roots were evenly spread across the planting area. The maximum L_v were 1.2 and 0.6 cm/cm³ and the estimated total root lengths per vine were 32 and 26 km for drip and microjet irrigated vines, respectively. Under drip irrigation, 56% of the variation in L_v could be accounted for as a function of depth and radial distance into the row, and under microjet, 45% of the variation in L_v could be accounted for as a function of depth. Twenty five per cent of the vine roots were in soil with an air filled porosity at field capacity of 6% or less. Based on the variation of root length per unit area (L_a) across a quarter of the planting area and between vines, we concluded that selection of a location at which the L_a would be representative of that in the entire irrigation unit is feasible in microjet irrigated vines but not in those irrigated with drip. The absence of a location representative of L_a confounds the scheduling of drip irrigation based solely on measurements of soil moisture.     

Growth and water use of eucalypt trees irrigated with saline drainage water

Leaf chemical composition, growth and water use of Eucalyptus camaldulensis (Lake Albacutya provenance) were measured in the 4th year of a split-plot salinity by nutrition trial. The main plot consisted of irrigating with five different water salinities: 0.5 dS/m (S0.5), 2 dS/m (S2), 5 dS/m (S5), 7.5 dS/m (S7.5) and 10 dS/m (S10). The subplot treatments consisted either of annual additions of 200 kg N and 100 kg P per hectare (+ N + P) or no addition of nutrients (– N – P). Irrigation with water from a drainage system (treatments S2, S5, S7.5 and S10) added about a further 100 kg N/ha annually. Leaf concentrations of N and P were higher in the + N + P treatments. In S0.5, nutrient addition stimulated growth. In + N + P treatments, raising the irrigation salinity from 0.5 to 2.0 dS/m increased leaf Na and decreased the growth rate, however, further increases in salinity affected neither leaf Na nor growth. In – N – P, growth rate depression due to inadequate nutrition was overcome in S2 and S5 by the 100 kg/ha of N in the drainage water. At higher salinities, the N added by drainage water did not overcome the effect of inadequate nutrition. On days when the reference crop evapotranspiration (ETo) was less than 3 mm/day, the correlations between water use of trees in litres per day and ETo and between water use of trees in litres per day and the basal area of the tree butt were highly significant. On days when the ETo was 3 mm/day or greater, the correlation between tree water use and basal area was highly significant, but that between tree water use and ETo was not significant. Received: 15 March 1996     

Biased signaling pathways in β2-adrenergic receptor characterized by 19F-NMR

Extracellular ligand binding to G protein-coupled receptors (GPCRs) modulates G protein and β-arrestin signaling by changing the conformational states of the cytoplasmic region of the receptor. Using site-specific (19)F-NMR (fluorine-19 nuclear magnetic resonance) labels in the β(2)-adrenergic receptor (β(2)AR) in complexes with various ligands, we observed that the cytoplasmic ends of helices VI and VII adopt two major conformational states. Changes in the NMR signals reveal that agonist binding primarily shifts the equilibrium toward the G protein-specific active state of helix VI. In contrast, β-arrestin-biased ligands predominantly impact the conformational states of helix VII. The selective effects of different ligands on the conformational equilibria involving helices VI and VII provide insights into the long-range structural plasticity of β(2)AR in partial and biased agonist signaling.     

Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists

Chemokine receptors are critical regulators of cell migration in the context of immune surveillance, inflammation, and development. The G protein-coupled chemokine receptor CXCR4 is specifically implicated in cancer metastasis and HIV-1 infection. Here we report five independent crystal structures of CXCR4 bound to an antagonist small molecule IT1t and a cyclic peptide CVX15 at 2.5 to 3.2 angstrom resolution. All structures reveal a consistent homodimer with an interface including helices V and VI that may be involved in regulating signaling. The location and shape of the ligand-binding sites differ from other G protein-coupled receptors and are closer to the extracellular surface. These structures provide new clues about the interactions between CXCR4 and its natural ligand CXCL12, and with the HIV-1 glycoprotein gp120.     

GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function

The beta2-adrenergic receptor (beta2AR) is a well-studied prototype for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) that respond to diffusible hormones and neurotransmitters. To overcome the structural flexibility of the beta2AR and to facilitate its crystallization, we engineered a beta2AR fusion protein in which T4 lysozyme (T4L) replaces most of the third intracellular loop of the GPCR ("beta2AR-T4L") and showed that this protein retains near-native pharmacologic properties. Analysis of adrenergic receptor ligand-binding mutants within the context of the reported high-resolution structure of beta2AR-T4L provides insights into inverse-agonist binding and the structural changes required to accommodate catecholamine agonists. Amino acids known to regulate receptor function are linked through packing interactions and a network of hydrogen bonds, suggesting a conformational pathway from the ligand-binding pocket to regions that interact with G proteins.     

Reduction of tropical cloudiness by soot

Measurements and models show that enhanced aerosol concentrations can augment cloud albedo not only by increasing total droplet cross-sectional area, but also by reducing precipitation and thereby increasing cloud water content and cloud coverage. Aerosol pollution is expected to exert a net cooling influence on the global climate through these conventional mechanisms. Here, we demonstrate an opposite mechanism through which aerosols can reduce cloud cover and thus significantly offset aerosol-induced radiative cooling at the top of the atmosphere on a regional scale. In model simulations, the daytime clearing of trade cumulus is hastened and intensified by solar heating in dark haze (as found over much of the northern Indian Ocean during the northeast monsoon).