Ectopic epithelial cell clusters in salmonid intestine are associated with inflammation

An epizootic incidence of intestinal adenocarcinomas was reported in brood fish of Atlantic salmon (Salmo salar L.) in 2009. The condition was associated with a specific diet inducing enteritis and morphological changes. Here, two field trials of fish up to slaughter size were initiated. In Trial 1, two different feed recipes were used. Feed I was predominantly based on marine ingredients, whereas plant ingredients were limited to soy protein concentrate and wheat. Feed II was lower in fishmeal and without soya protein, which was substituted with plant proteins from other sources. In Trial 2, a commercial feed (Feed III) was included. No macroscopic tumours were observed in 300 fish (Trial 1). At the end of both trials, samples from five different segments of the gastrointestinal tract of a total of 39 fish were investigated with morphological methods. Here, we show the presence of ectopic proliferating epithelial cells only occurring in inflamed intestine and predominantly in the second segment of the mid‐intestine. Presence of ectopic epithelial cells in submucosal inflammatory foci may indicate early stages in tumorigenesis, but other possibilities such as proliferative enteric disorders cannot be excluded. Together with inflammation, carcinogenesis should be a focus of investigation in future feed trials.     

Impact of Pseudomonas H6 surfactant on all external life cycle stages of the fish parasitic ciliate Ichthyophthirius multifiliis

A bacterial biosurfactant isolated from Pseudomonas (strain H6) has previously been shown to have a lethal effect on the oomycete Saprolegnia diclina infecting fish eggs. The present work demonstrates that the same biosurfactant has a strong in vitro antiparasitic effect on the fish pathogenic ciliate Ichthyophthirius multifiliis. Three life cycle stages (the infective theront stage, the tomont and the tomocyst containing tomites) were all susceptible to the surfactant. Theronts were the most sensitive showing 100% mortality in as low concentrations as 10 and 13 μg/ml within 30 min. Tomonts were the most resistant but were killed in concentrations of 100 μg/ml. Tomocysts, which generally are considered resistant to chemical and medical treatment, due to the surrounding protective cyst wall, were also sensitive. The surfactant, in concentrations of 10 and 13 μg/ml, penetrated the cyst wall and killed the enclosed tomites within 60 min. Rainbow trout fingerlings exposed to the biosurfactant showed no adverse immediate or late signs following several hours incubation in concentrations effective for killing the parasite. This bacterial surfactant may be further developed for application as an antiparasitic control agent in aquaculture.     

Visitor preferences of thinning practice in young even-aged stands of pedunculate oak (Quercus robur L.): comparing the opinion of forestry professionals in six European countries

This study compared visitor preferences of forestry professionals across six European countries (Sweden, Denmark, Great Britain, Austria, Romania and Portugal) using a questionnaire survey. The 598 interviewees were asked to rank photographs depicting recently thinned experimental plots in a 13-year old stand of pedunculate oak (Quercus robur L.) according to the criterion: “Which forest environment do you prefer as a visitor?” The plots represented five different residual stem densities: 7000 (no thinning, very high stem density), 5300 (heavy thinning, high stem density), 1000 (very heavy thinning, medium stem density), 300 (extremely heavy thinning, low stem density/open stand) and 100 (solitary trees, very low stem density/very open stand) stems ha^?1. The results indicated geographical variation in the preferences for different thinning practices in young stands of oak. Portuguese, Austrian and Romanian respondents generally favoured thinned, but dense stands, whereas Danish and British respondents preferred very heavily thinned stands. Swedish respondents preferred open stands resulting from extremely heavy thinning. Photographs taken along rows were favoured to photographs across rows, indicating a preference for scenes offering perspective and accessibility. The results indicate a variation of visitor preferences among forestry professionals for different silvicultural regimes. We interpret this in the context of national traditions and forestry paradigms that influence the shaping of preferences.     

Application of sorbents in different soil types from small arms shooting ranges for immobilization of lead (Pb), copper (Cu), zinc (Zn), and antimony (Sb)

Journal of Soils and Sediments - Soil contamination of ammunition residues at shooting ranges for small arms may be followed by leaching of lead (Pb), copper (Cu), zinc (Zn), and antimony (Sb)....     

Trends and drivers of on-farm conservation of the root legume ahipa (<Emphasis Type="Italic">Pachyrhizus ahipa</Emphasis>) in Bolivia over the period 1994/96–2012

The leguminous Andean root crop ahipa has become scarce and the current status of in situ conservation in Bolivia is concerning. Agro-biodiversity changes affect the use, conservation and socio-economic trends for ahipa (Pachyrhizus ahipa) and to substantiate this, a comparison of two cropping periods were made. Aspects of root production and the shift from cultivation of ahipa to cash crops such as maize were compared for two periods: (a) 1994/96 and (b) 2012. Our study showed that the price of ahipa had not increased in 70% of the urban markets; hence, there is little incentive to retain the cultivation of ahipa. We found that rural ahipa growers selected the largest seeds, but did not select seeds by colour. The mixed seed colour predominated the conservation of ahipa. Laborious yield enhancing practices required in field management and low market value endanger future conservation of the ahipa. We conclude that additional research is needed in order to safeguard the extant variation of ahipa, i.e. nutritional value, taste, stress tolerance and market potential.     

Farm and operator characteristics affecting adoption of precision agriculture in Denmark and Germany

Precision Agriculture (PA) has been advocated as a promising technology and management philosophy that provides multidimensional benefits for producers and consumers while being environmentally friendly. In Europe, private stakeholders (farm advisors, farm equipment producers, decision support providers, farmers) and research institutions have been trying to develop, test and demonstrate adoption of precision agriculture solutions with governments financing big projects in these areas. Despite these efforts, adoption is still lagging behind expectations.

Whether farmers adopt PA or not is likely to be influenced by several factors. This study intends to identify the main socio-economic determinants of adoption of precision agriculture in Denmark and Germany employing a binary logit model on a cross-section survey data. The results show that farm size, farmer age and demonstration and networking events like attending workshops and exhibitions significantly influence farmers’ adoption decision.     

Apple rootstocks may become infected by Neonectria ditissima during propagation

Introduction and purpose: The ability of apple rootstocks to become infected by Neonectria ditissima, the cause of European canker, was studied over two years.

Materials and methods: Rootstocks B9 and M9 with a size suitable for grafting (6-10 mm stem diameter, termed rootstocks), and smaller sized rootstocks (<5 mm stem diameter, termed transplants) of B9, M9, M26, MM106 and Antonovka were inoculated with N. ditissima at different times, either with contaminated map pins or with spore suspensions. In addition, the rootstocks were either defeathered (side shoots removed), topped (top shoot headed) or both, to create wounds that would normally occur during propagation, while wounds on transplants were made by removing leaves.

Results and discussion: One month after inoculation, slightly sunken canker lesions had developed around the inoculation points of the map pins or wounds. No lesions developed on the non-inoculated controls. Map pin inoculation resulted in 30% to 89% infection and spore suspension sprayed on wounds from 5% to 45% infection. When the cankered areas were split open, brown lesions with necrotic tissue due to infection by N. ditissima appeared. The transplants of M9, M26 and MM106 inoculated with contaminated map pins in 2014 developed necrosis on 40% to 67% of the plants, but there were no differences in the incidence or severity among the different types. On the transplants of B9, Antonovka and M9 inoculated in 2015, there was more necrosis on B9 (42%) than on Antonovka (11%) and more sporulating lesions on B9 (29%) than on M9 (9%) or on Antonovka (4%).

Conclusion: It can be concluded that rootstocks used for apple trees may become infected by N. ditissima, and wounds should thus be protected during propagation.     

Haematogenous septic arthritis in foals: Short‐ and long‐term outcome and analysis of factors affecting prognosis

Synovial sepsis represents a major cause of morbidity and mortality in foals; however, there are no studies focusing on foals particularly at risk during the first 2 months of life. Our objective was to analyse outcome in foals aged <2 months with haematogenous septic arthritis and to identify prognostic factors and compare the effects of two different lavage procedures on outcome. Sixty foals with synovial sepsis were used for our retrospective study in which medical records, online data and telephone interviews were utilised to analyse prognostic factors, and determine short‐ and long‐term survival and athletic performance of foals treated at Evidensia Equine Specialist Hospital, Helsingborg, between 2008 and 2014. Overall survival to discharge was 80% (48/60). Time to long‐term follow‐up ranged from 6 months to 6.5 years. Forty/60 (67%) horses survived long‐term. Four/8 (50%) of the nonsurvivors at long‐term follow‐up had been subjected to euthanasia due to sequelae of joint sepsis. Of the long‐term survivors, 37/40 (92.5%) achieved athletic soundness. Overall long‐term outcome for athletic soundness was 37/60 (62%). Prognostic factors based on historical, clinical or laboratory data, or the use of through‐and‐through needle lavage compared to endoscopic lavage as the first procedure, could not be identified. The present study demonstrates a more favourable prognosis than previously reported, with 80% of foals with synovial sepsis surviving to discharge, and approximately 60% achieving athletic soundness. However, the study was limited by a small population size, which may account for the failure to verify statistically significant prognostic factors.