Red alder (Alnus rubra) alters community-level soil microbial function in conifer forests of the Pacific Northwest, USA

Nitrogen-fixing tree species have been shown to improve site fertility and increase N transformation rates, but the influence of N-fixing plants on the soil microbial community as a whole is largely unknown. We used patterns of individual carbon-source utilization and enzyme activities to assess the relative effects of N-fixing red alder on the soil microbial community in three adjacent stands (pure conifer, mixed alder-conifer, and pure alder) of a highly productive coastal Oregon forest where the density of red alder has been experimentally manipulated for over 65 years. Two major patterns were revealed: (1) bacterial and fungal carbon-source utilization patterns in soil from pure conifer stands were significantly different from both pure alder soils and mixed conifer-alder soils, while there was no difference in substrate utilization patterns between soils from the mixed alder-conifer and pure alder stands; and (2) the activities of nine extracellular enzymes involved in ligno-cellulose degradation and the mineralization of organic nitrogen, phosphate, and sulfate compounds were all significantly greater in pure alder soils compared to either pure conifer or mixed conifer-alder soils. Our results show that, in addition to an overall increase in soil fertility, microbial biomass, and microbial activity, the presence of N-fixing red alder significantly alters the physiological profile of the microbial community-even in an ecosystem already of high N status.     

Characterization of the triterpene saponins of the roots and rhizomes of blue cohosh (Caulophyllum thalictroides).

Seven triterpene saponins were isolated from n-butanol fractions of blue cohosh (Caulophyllum thalictroides) roots and rhizomes. Their structures were established by spectral ((1)H NMR, (13)C NMR, 2D-NMR, and APCI-MS) techniques and chemical reactions as hederagenin 3-O-alpha-L-arabinopyranoside (1); caulophyllogenin 3-O-alpha-L-arabinopyranoside (2); hederagenin 3-O-beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinopyranoside (3); 3-O-alpha-L-arabinopyranosyl-hederagenin 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranoside (4); 3-O-alpha-L-arabinopyranosyl- caulophyllogenin 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranoside (5); 3-O-beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinopyranosyl- echinocystic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranoside (6); 3-O-beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinopyranosyl-hederagenin 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranoside (7). All seven compounds were identified in this species for the first time.     

Differential Rotation and Dynamics of the Solar Interior

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.     

Isolation, structure determination, synthesis, and sensory activity of N-phenylpropenoyl-L-amino acids from cocoa (Theobroma cacao)

Application of chromatographic separation and taste dilution analyses recently revealed besides procyanidins a series of N-phenylpropenoyl amino acids as the key contributors to the astringent taste of nonfermented cocoa beans as well as roasted cocoa nibs. Because these amides have as yet not been reported as key taste compounds, this paper presents the isolation, structure determination, and sensory activity of these amino acid amides. Besides the previously reported (-)-N-[3',4'-dihydroxy-(E)-cinnamoyl]-3-hydroxy-L-tyrosine (clovamide), (-)-N-[4'-hydroxy-(E)-cinnamoyl]-L-tyrosine (deoxyclovamide), and (-)-N-[3',4'-dihydroxy-(E)-cinnamoyl]-L-tyrosine, seven additional amides, namely, (+)-N-[3',4'-dihydroxy-(E)-cinnamoyl]-L-aspartic acid, (+)-N-[4'-hydroxy-(E)-cinnamoyl]-L-aspartic acid, (-)-N-[3',4'-dihydroxy-(E)-cinnamoyl]-L-glutamic acid, (-)-N-[4'-hydroxy-(E)-cinnamoyl]-L-glutamic acid, (-)-N-[4'-hydroxy-(E)-cinnamoyl]-3-hydroxy-L-tyrosine, (+)-N-[4'-hydroxy-3'-methoxy-(E)-cinnamoyl]-L-aspartic acid, and (+)-N-[(E)-cinnamoyl]-L-aspartic acid, were identified for the first time in cocoa products by means of LC-MS/MS, 1D/2D-NMR, UV-vis, CD spectroscopy, and polarimetry, as well as independent enantiopure synthesis. Using the recently developed half-tongue test, human recognition thresholds for the astringent and mouth-drying oral sensation were determined to be between 26 and 220 micromol/L (water) depending on the amino acid moiety. In addition, exposure to light rapidly converted these [E]-configured N-phenylpropenoyl amino acids into the corresponding [Z]-isomers, thus indicating that analysis of these compounds in food and plant materials needs to be performed very carefully in the absence of light to prevent artifact formation.     

Interactive effects of nitrogen and phosphorus on verticillium wilt of Russet Burbank potato

A three-year field study was conducted during 1984–1986 to determine the interactive effects of nitrogen and phosphorus fertilization on Verticillium wilt, and yield and quality of continuously-cropped Russet Burbank potato. The experiment was conducted on a calcareous, silt loam soil with low initial levels of NO₃-N (0.9 mg/kg), P (3.5 mg/kg), andVerticillium dahliae (9 cfu/g of soil). The experimental design consisted of a factorial combination of three N treatments (unfertilized check, preplant N or split N) and three P treatments (0,120, or 240 kg P/ha) applied to the same plots during the three-year study. Nitrogen was applied at 0 or 300 kg N/ha in 1984 and 0 or 240 kg N/ha in 1985 and 1986. By the spring of 1986, soil P concentrations for the 0,120, and 240 kg P/ha treatments had increased to 7, 25, and 50 mg/kg and no additional P was applied. In addition to suppressing Verticillium wilt by as much as 95%, N & P treatments also reduced the rate of increase of soilborne inoculum ofV. dahliae. After one season of cropping, the N treatment providing the most efficient N fertilization (300 kg N/ha, split-application) resulted in significantly (p=0.01) lowerV. dahliae counts in soil than the other N treatments. After two seasons of continuous cropping, applying 120 to 240 kg P/ha produced lower populations ofV. dahliae in soil compared to the treatment with no added P. Generally, as N and P treatments approached the highest levels, both wilt incidence andV. dahliae colonization values were reduced. There was a progressive reduction in total yield in nearly all treatments during each year of continuous cropping. Reduction of yield and increased Verticillium wilt incidence was generally greater with N or P deficient plants than with optimally fertilized plants. The highest total and U.S. No. 1 yields and least Verticillium wilt were obtained each year by applying split N and 240 kg P/ha. Results show that optimal N and P can minimize both Verticillium wilt and yield losses that normally occur with intensive potato cropping.     

Cultivar and seedpiece spacing effects on potato competitiveness with weeds

Field studies were conducted in 1991 and 1992 to evaluate the effects of cultivar, row spacing, and within-row spacing on potato yield and quality under weedy and weed-free conditions. Cultivars tested were Russet Burbank, an indeterminate, large-vined cultivar, and Frontier Russet, a determinate, small-vined cultivar. The two cultivars were grown under weedy and weedfree conditions with either 76 or 91 cm row spacings in factorial combination with either 15, 25, or 35 cm within-row spacings. The major competitive weeds were redroot pigweed, common lambsquarter and hairy nightshade. The weedy plots consistently produced less vine and tuber biomass and less total and U.S. No. 1 tuber yield than the weed-free plots. The time of weed emergence strongly affected potato competitiveness with weeds. In 1991, weeds emerged after potatoes, giving the crop some competitive advantage. In 1992, weeds emerged before the potatoes, resulting in heavy competition and large decreases in vine and tuber production for both cultivars. Reductions in U.S. No. 1 tuber yield were proportionally greater than the reductions in total yield. Weedy plots in 1991 and 1992 produced 25% and 68% less total yield and 43% and 92% less U.S. No. 1 yield, respectively, than weed-free plots. Russet Burbank was more competitive with weeds than Frontier Russet. Frontier Russet suffered substantial losses in productivity due to the presence of weeds, even under moderate weed pressure in 1991. Decreasing the row width from 91 to 76 cm did not provide a competitive advantage for potatoes as measured by vine or tuber biomass, or tuber yield. Decreasing within-row spacing under weedy conditions provided some competitive advantage and resulted in higher vine and tuber biomass and greater total tuber yield. The closer within-row spacing resulted in a substantial decrease in U.S. No. 1 yield with Russet Burbank but a slight increase with Frontier Russet. There were several significant interactions involving cultivar, weed level, and within-row spacing. These were due, in part, to each cultivar’s unique response to inter-and intraspecies competition. Cultivar had a greater influence on competitiveness than any plant spatial arrangement.     

Optimal allocation of limited water supplies for Russet Burbank potatoes

Water shortages in many potato-producing regions have increased the demand for information on irrigating potatoes with limited water supplies. Field studies were conducted at Aberdeen, Idaho in 1988 and 1989 to determine the optimal allocation of limited water supplies for Russet Burbank potatoes. Irrigation amounts equal to 60 or 80% of estimated seasonal evapotranspiration (ET) were applied using various patterns of water allocation. Irrigation deficits were either partitioned evenly over the entire tuber bulking period, or were imposed during two of three designated growth stages (early, mid or late bulking) corresponding to the periods from 0 to 3, 3 to 6, and 6 to 9 weeks after tuber initiation, respectively. A well-watered, 100% ET check was included for comparison. Total yield reductions were greater when irrigation deficits were imposed during the early-mid and midlate bulking sequences than when they were imposed evenly over the entire tuber bulking period or during the early-late bulking sequence. Results relating U.S. No. 1 yields to seasonal water allocation patterns were similar to those for total yield. However, U.S. No. 1 yield reductions were proportionately greater. Irrigation deficits imposed during the early-mid bulking sequence resulted in the lowest specific gravities and the highest percentages of dark ends.     

Potato nutrient management in sustainable cropping systems

Sustainable nutrient management involves a set of management practices designed to conserve soil resources, to maintain or enhance productivity, and to help reduce growers’ reliance on chemical fertilizers. Sustainable nutrient management systems will most certainly rely heavily on the use of legume rotation crops and/or organic soil amendments. To realize the full benefit to the crop ecosystem, sustainable nutrient management programs will also likely require longer crop rotations with less frequent potato production. There is considerable research evidence indicating that these systems can maintain or increase productivity while having positive impacts on nutrient supply, soil physical properties, and soil conservation. However, their successful adoption and continued use on a large scale will require resolution of uncertainties related to economic risk associated with inconsistent nutrient availability in alternative nutrient management systems, potential environmental risks associated with excessive P applications in animal manures, and the potential for increased potato pest incidence resulting from manure application.