A comparison of geographical and neighbourhood models for improving atlas databases. The case of the French butterfly atlas

We use data from the French national butterfly atlas to compare the potential of direct geographical and neighbourhood models to account for numbers of species and incidence of species in French départements. Direct geographical models use data on latitude, longitude and altitude, whereas neighbourhood models use information from adjacent areas. Both geographical models and neighbourhood models account for a large proportion of the variance in species richness (68-78%). However, neighbourhood models are more successful than models based solely on simple geographical variables. A large number of individual species distributions are accounted for by logistic and autologistic regression models (222 of 246 species, 90.2%). The autologistic models incorporate information on neighbouring areas. The exceptions are rare species, five of six of which occur in a single administrative unit only (2.4%), or virtually ubiquitous species found in >90% of units (7.3%). Autologistic models dominate logistic models in accounting for species incidences using stepwise logit regressions, neighbourhood variables appearing in 64.5% of successful species models (absent in 22.8%) and then always entering first. A simple neighbourhood (distance-unweighted) measure (C₂) dominates more models (89 of 246 species, 36.2%) than a distance-weighted neighbourhood measure (C₁; 77 of 246 species, 31.3%). The models are here demonstrated to be potentially valuable for identifying under-recording and losses from regional extinction and for filling gaps in recording. The findings reveal substantial, apparent, losses of species in western and northern France as well as substantial discrepancies (differences) in numbers of species, for some administrative units (départements) and for both post-1970 and total records, compared with numbers predicted to occur. We use two distinct approaches on total species and individual species to provide comparative estimates of the numbers of species expected within spatial units and we present the number of additional units in which species are expected to occur. The probabilities for these species in French départements are available on Web site: http://www.brookes.ac.uk/schools/bms/research/data/ecology/butterfly.html.     

Sulphate adsorption at the Fe (hydr)oxide–H2O interface: comparison of cluster and periodic slab DFT predictions

The transport and bioavailability of sulphate in soils are significantly affected by adsorption reactions at the mineral–H₂O interface. Therefore, an understanding of the mechanisms and kinetics of sulphate adsorption is of fundamental importance in soil chemistry. In this investigation, the binding geometries of bidentate bridging and monodentate sulphate complexes at the Fe (hydr)oxide–H₂O interface were predicted with static cluster and periodic slab density functional theory (DFT) calculations. The cluster calculations were performed with edge‐sharing dioctahedral Fe³⁺ models, using the unrestricted PBE0 exchange‐correlation functional and a combination of effective core potential (LanL2DZ – Fe atoms) and all‐electron (6–311+G(d,p) or 6–311+G(3df,p) – S, O, and H atoms) basis sets. The periodic slab calculations were performed with a (3 × 2) slab of the (100) α‐FeOOH surface, by means of the projector‐augmented wave method and a plane‐wave basis set. For the periodic slab DFT calculations, the spin‐polarized (SP) PBE exchange‐correlation functional, with and without explicit consideration of an on‐site Coulomb interaction parameter (i.e. SP‐PBE and SP‐PBE+U methods), was used. Despite the lack of long‐range order, cluster model predictions of the interatomic distances and angles of bidentate bridging and monodentate sulphate were in good agreement with the periodic slab model predictions. Quantitative analysis of the cluster and periodic slab DFT predictions is expected to result from theoretical fitting of extended X‐ray absorption fine structure measurements. The application of computational chemistry methods to soil chemistry research is anticipated to provide novel insight into the mechanisms and kinetics of ion sorption.     

Barn owl release in lowland southern England—a twenty-one year study

Twenty-one years of carefully documented barn owl (Tyto alba) study and release in England by the South Midlands Barn Owl Conservation Group (SMBOCG) have necessitated a re-examination of some of the premises on which such release schemes are judged. Fifteen years after the first releases, the number of independently breeding owls (i.e. breeding ‘wild’ or ‘as though wild’) in a 1200 km² study area is still increasing sharply and now far exceeds the number released in any one year, with the rate of increase outstripping the cumulative number of release events. Ringing returns indicate that fledged young dispersed further than adults, and that released adults which immediately deserted their release sites were less likely to survive their first 30 days than those staying in the vicinity of their release, where they could take advantage of supplementary feeding. Artificial feeding at release sites led to pellets containing lower liveweight equivalent of wild-caught prey but higher numbers of fledged young. Otherwise, and importantly, no significant difference in survival, mortality, dispersal, foraging success or breeding success was found between wild/independent owls and various categories of released bird. Availability of field vole habitat was not as important for barn owl breeding success as was found in previous studies, with owls able to replace this species in their diet with the less habitat-specific wood mouse. Evidence is provided that starvation was not as important a cause of mortality as has been proved elsewhere. Although barn owl nests often occurred in extreme close proximity, breeding density had no effect on either foraging or breeding success. The possible genetic consequences of barn owl release in Britain are briefly discussed. Very few barn owl release schemes have attempted to prove that their work is worthwhile, so long-term, well-documented studies such as that presented here are vital in establishing some of the principles involved in releasing barn owls into the wild.     

Spatial and temporal controls of soil respiration rate in a high-elevation, subalpine forest

We examined soil respiration to determine what measurable environmental variables can be used to predict variation in soil respiration rates, spatially and temporally, at a high-elevation, mixed conifer, subalpine forest site at the Niwot Ridge Ameriflux Site in Colorado. For three summers, soil respiration rates were measured using soil collars and a portable gas-exchange system. Transects of the collars were established to ensure spatial characterization of the litter-repleted areas beneath tree crowns and the litter-depleted open spaces between tree crowns. Soil temperature and soil moisture were both identified as important drivers of soil respiration rate, but were found to confound each other and to function as primary controls at different scales. Soil temperature represents a primary control seasonally, and soil moisture represents a primary control interannually. Spatially, organic layer thickness, ammonium concentration, water content, and the microbial and soil soluble carbon pools were found to predict variation from point to point. Soil microbial biomass strongly correlated to soil respiration rate, whereas root biomass was identified as a weak predictor of respiration rate and only when controlling for other variables. Spatial variation in soil respiration rate is highly determined by the depth of the soil organic horizon, which in this ecosystem varies predictably according to distance from trees. The conclusions that can be drawn from the study provide the foundation for the development of future models of soil respiration driven by fundamental variables of the climate and soil microenvironment.     

Potassium-salinity interactions in irrigated corn

Summary Potassium uptake by plants can be affected by high salinity and the Na concentration in the soil solution. There is abundant evidence that Na and the Na/Ca ratio affects K uptake and accumulation within plant cells and organs and that salt tolerance is correlated with selectivity for K uptake over Na. This provides the basis for hypothesis which exists in the literature and was examined in this study, that K application can reduce salinity damage to plants. The main objectives of this study were to: (i) study the effects of salinity and K fertilization interactions on corn yield and nutrient uptake; (ii) test the possibility that salinity damage can be reduced by elevating K fertilization rate; and (iii) study K dynamics in soil as a function of the salinity of the irrigation water, in soils with high and low indigenous potassium. The response of corn (Zea mays (L.) cv. Jubilee) to K fertilization under saline and non-saline conditions was studied by growing corn in two soil types in a pot experiment. Rates of K application to a 3 kg pot were: 0, 15 and 30 mmol K to the Gilat soil and 7.5, 15 and 30 mmol K to the Nordiya soil as KCl. The desired quantity of K was applied in one dose after seedling emergence. The salinity levels of the irrigation water were 4, 20 and 40 mmol charge 1^–1. The irrigation was applied at least every second day and in excess to avoid water stress and to ensure drainage. Increased salinity in the irrigation water significantly decreased yield in both soils. Potassium significantly increased yield at all salinity levels only in the sandy soil which had a low natural level of K, but there was no difference in the relative yield decrease with salinity increase between the lowest and highest K application rates. Potassium fertilization did not eliminate the deleterious effects of salinity on corn yield despite its beneficial effect of increasing K content and reducing the NaK ratio in plant tissue. Potassium uptake by plants was the major factor in K dynamic processes. Potassium adsorption, release and fixation were secondary factors while leaching was an insignificant factor in overall K balance under cropping conditions.     

Simulation of the economic impact of proliferative enteritis on pig production in Australia

The economic impact of proliferative enteritis (PE) on an ‘average’ pig farm was calculated using the AUSPIG decision support system. Inputs were modelled on actual cases of PE, in which affected herds suffered from depressed growth rate, decreased feed efficiency and stock losses. The costs associated with non-haemorrhagic PE and proliferative haemorrhagic enteropathy ranged from $15/sow/yr to $141/sow/yr, respectively, depending on the clinical severity of the disease, incidence of infection and the type of medication strategy used to treat and control the disease.     

Changes in the botanical diversity of a species rich ancient hedgerow between two surveys (1971-1998)

This paper reports on a unique resurvey of the composition of a species rich ancient hedgerow in Cambridgeshire, UK, 27 years after the original study. The hedge contained sections that were unmanaged and unaffected by agrochemicals, as well as sections which were cut annually, adjacent to an intensively managed arable field. Between the two surveys there was a general decline in species richness in all sections of the hedge, particularly in ground flora normally associated with ancient woodland. A mean of 0.42 woody species per 30-m length of hedgerow was lost in 27 years. Contrary to a priori expectation, there was no suggestion that damage was greater in the farmed section. These results highlight that zero management is not an appropriate option for maintaining the floral diversity of species rich and ancient hedgerows.     

Trace chemicals: the essence of sexual communication systems in heliothis species

Analysis of heptane-soluble compounds from ovipositors of Heliothis zea and Heliothis virescens shows that both species produce relatively large amounts of (Z)-11-hexadecenal, with traces of (Z)-9-hexadecenal, (Z)-7-hexadecenal, and hexadecanal. Heliothis virescens females differ from Heliothis zea in that they also produce trace amounts of tetradecanal, (Z)-9-tetradecenal, and (Z)-11-hexadecen-1-ol. In both species, trace compounds are essential to pheromonal activity and specificity of chemical signals.     

Activation of Zona‐Free Buffalo (Bubalus bubalis) Oocytes by Chemical or Electrical stimulation,and Subsequent Parthenogenetic Embryo Development

Parthenogenetic activation using zona‐free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. The aim of this study was to compare the efficacy of different methods for the activation of zona‐free buffalo oocytes in terms of their effects on the developmental competence of parthenogenetic embryos. The effects of zona removal on parthenogenetic activation and in vitro developmental competence of metaphase II oocytes were also examined. All activation methods were followed by incubation of 2 mm 6‐dimethylaminopurine (6‐DMAP) for 4 h. Out of three different pulse strengths (1.2, 2.1 or 3.3 kV/cm) used, 2.1 kV/cm resulted in the highest blastocyst rate (25.3%). On comparing different chemical agents and electric pulse, highest blastocyst rate was observed for calcium ionophore (CaI) (28.6%) followed by ethanol (25.0%), electric pulse (22.5%) and combined CaI and ethanol treatment (16.7%) although differences among them were not significant. Furthermore, a significantly reduced developmental potential was observed in zona‐free oocytes when compared to zona‐intact ones up to the blastocyst stage (44.3% vs 27.1%). In conclusion, zona‐free buffalo oocytes can be successfully activated for parthenogenetic development using chemical or electrical stimulation. Out of different agents examined, CaI followed by 6‐DMAP resulted in the highest blastocyst rate.