Estimation of the relative bioavailability of copper sources in chicks fed on conventional dietary amounts

1. Tissue accumulation of Cu from dietary additions of 0, 5, 10, or 20 mg/kg Cu as reagent grade Cu acetate and feed grade Cu carbonate was determined in day‐old chicks fed on conventional maize‐soyabean meal starter diets (5.41 mg/kg Cu as‐fed basis) for 3 weeks.

2. Average daily food intake, daily weight gain and food conversion were similar among treatments.

3. There were linear increases in plasma and liver Cu concentrations (P< 0.01) as dietary Cu increased.

4. Bioavailability of Cu as carbonate was 0.66 that of Cu in the acetate based on the multiple regression slope ratio of liver Cu concentration on added dietary Cu. Although responses for the two Cu sources did not differ significantly, the relative bioavailability of the Cu carbonate was similar (0.66 vs 0.68) to that obtained in an earlier study (Ledoux et al., 1991) with greater dietary Cu contents (150, 300 and 450 mg/kg) in which the slopes of the equations representing the two sources differed (P<0.05).     

Relationship between equivalent salt solution series of different soils

Equivalent salt solution series have been previously defined as solutions with combinations of sodium absorption ratio (SAR) and electrolyte concentration (E_c) producing the same extent of clay swelling in a given soil. The present study shows that there is a high (r²>0.96) positive correlation between log E_c and log SAR of equivalent salt solutions series, in the equation: where a₁ and b₁ are constants for each equivalent salt solution series for a given soil. Log a₁ could also be represented as a linear function of b₁ resulting in the equation: where a₂ and b₂ are constants for a given soil. Solving this equation using any given value of b₁ yields the combinations of SAR and E_c which make up each equivalent salt solution series for a given soil. The relationship between log a₁ and b₂ for three soils from western United States, namely Waukena, Pachappa and Grangeville, was similar, with their combined data having a r² value of 0.96. This indicated that a single set of equivalent salt solution series values could be used for these three soils which have different clay contents and clay mineralogy. Prediction of hydraulic conductivity decreases with E_c reduction at given values of SAR in red-brown and alluvial soils from southern Tasmania, using the equivalent salt solution series values for Waukena soil, showed similar patterns to measured values and also to those predicted using the equivalent salt solution values applicable to the respective Tasmanian soils. Thus, available data indicate that the same set of equivalent salt series could be applied to the five soils studied. If further testing shows that a single set of equivalent salt solutions values could be applied to all or large groups of soils, this would facilitate the application of the equivalent salt solution concept to predict salt solution flow in the field.     

Atomic-resolution electrochemistry with the atomic force microscope: copper deposition on gold

The atomic force microscope (AFM) was used to image an electrode surface at atomic resolution while the electrode was under potential control in a fluid electrolyte. A new level of subtlety was observed for each step of a complete electrochemical cycle that started with an Au(111) surface onto which bulk Cu was electrodeposited. The Cu was stripped down to an underpotential-deposited monolayer and finally returned to a bare Au(111) surface. The images revealed that the underpotential-deposited monolayer has different structures in different electrolytes. Specifically, for a perchloric acid electrolyte the Cu atoms are in a close-packed lattice with a spacing of 0.29 +/- 0.02 nanometer (nm). For a sulfate electrolyte they are in a more open lattice with a spacing of 0.49 +/- 0.02 nm. As the deposited Cu layer grew thicker, the Cu atoms converged to a (111)-oriented layer with a lattice spacing of 0.26 +/- 0.02 nm for both electrolytes. A terrace pattern was observed during dissolution of bulk Cu. Images were obtained of an atomically resolved Cu monolayer in one region and an atomically resolved Au substrate in another in which a 30 degrees rotation of the Cu monolayer lattice from the Au lattice is clearly visible.