Genetic Diversity in Accessions of Wild Rice Oryza granulata from South and Southeast Asia

Oryza granulata, an upland wild rice species, represents an unique germplasm for possessing abilities of tolerance to shade and drought, immune to bacterial blight and resistance to brown planthopper. Although low degree of genetic variability has been revealed within its populations, little genetic information at the species level is available in determining rational conservation strategies. Here we used dominant DNA marker random amplified polymorphism DNA (RAPD) to assess the genetic variability among 23 accessions of O. granulata that collected from main distribution areas worldwide. Twenty decamer primers generated a total of 243 bands, with 83.5% of them (203 bands) being polymorphic. Calculation of Shannon index of diversity revealed an average value of 0.42 ± 0.25, indicating that O. granulata maintains a relatively high degree of genetic diversity on the species level. Analysis of genetic dissimilarity (GD) showed that genetic differentiation occurred among studied accessions, which supports the feasibility of current ex situ conservation strategies. We also suggested that information based on population studies, which could be achieved by international co-operation, is needed to conserve this widespread germplasm more effectively.     

Multi-Step Competitive Sorption and Desorption of Chlorophenols in Surfactant Modified Montmorillonite

Single- and bi-solute sorption and desorption of 2,4-dichlorophenol (2,4-DCP) and 2,4,5-trichlorophenol (2,4,5-TCP) in montmorillonite modified with hexadecyltrimethylammonium (HDTMA) were investigated using multi-step sorption and desorption procedure. Effect of pH on the multi-step sorption and desorption was investigated. As expected by the magnitude of octanol-water partition coefficient, K _ow , both sorption and desorption affinity of 2,4,5-TCP was higher than that of 2,4-DCP at pH 4.85 and 9.15. For both chlorophenols, the protonated speciation (at pH 4.85) exhibited a higher affinity in both sorption and desorption than the predominant deprotonated speciation (about 95% and 99% of 2,4-dichlorophenolate and 2,4,5-trichlophenolate anions at pH 9.15, respectively). Desorption of chlorinated phenols was strongly dependent on the current pH regardless of their speciation in the previous sorption stage. Freundlich model was used to analyze the single-solute sorption and desorption data. No appreciable desorption-resistant (or non-desorbing) fraction was observed in organoclays after several multi-step desorptions. This indicates that sorption of phenols in organoclay mainly occurs via partitioning into the core of the pseudo-organic medium, thereby causing desorption nearly reversible. In bisolute competitive systems, sorption (or desorption) affinity of both chlorophenols was reduced compared to that in its single-solute system due to the competition between the solutes. The ideal adsorbed solution theory (IAST) coupled to the single-solute Freundlich model successfully predicted bisolute multi-step competitive sorption and desorption equilibria.     

山西省土地资源数据库的建立与研究

为了按时完成山西省水土保持规划任务,全省采用单公里网布点到位实测的数理统计法,同步查清了土地利用和治理现状,并将获得的80多万个数据输入IBM—PC/XT计算机,研制山西省土地资源数据库软件。本软件采用汉字显示,可以打印出所需的行政区和流域的数据项和图,为山西省水土保持工作使用计算机管理创造了良好的开端。     

Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.), maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.), and faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.)

Cereal/cereal and cereal/legume intercropping systems are popular in the north, northwest, and southwest of China and often result in yield increases compared to monocropping. Rhizosphere interactions may play a significant role in the yield increases, particularly with respect to nutrient availability. The aim of this study was to investigate the effects of intercropping on N availability and community composition of ammonia-oxidizing bacteria in the rhizosphere of wheat, maize, and faba bean at different growth stages. Denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes was used to analyze the community composition of bacterial ammonia oxidizers belonging to β-proteobacteria. The results showed that intercropping with faba bean significantly increased nitrate concentrations in the rhizosphere of wheat and maize at the second sampling time (20 June) compared to monocropping or intercropping between maize and wheat. Intercropping significantly affected the community composition of ammonia-oxidizing bacteria in the rhizosphere compared to monocropping, and the effects were most pronounced in the maize/faba bean and wheat/maize intercropping systems when faba bean and wheat were at anthesis and maize was in seedling stage. In wheat/faba bean intercropping, the effects of intercropping on community composition of ammonia-oxidizing bacteria were less pronounced at the seedling stage of the two species but were significant at anthesis.     

Development of EST-SSR in foxtail millet (<Emphasis Type="Italic">Setaria italica</Emphasis>)

The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic and transferable SSRs were also identified.     

Predicting the Phytoextraction Duration to Remediate Heavy Metal Contaminated Soils

The applicability of phytoextraction to remediate soils contaminated with heavy metals (HMs) depends on, amongst others, the duration before remediation is completed. The impact of changes in the HM content in soil occurring during remediation on plant uptake has to be considered in order to obtain a reliable estimate of the phytoextraction duration. To simulate the decrease in the HM content in soil and to assess the resulting decrease in the uptake of HMs by plants, contaminated soil was mixed with uncontaminated, but otherwise similar soil. Uptake of Cd, Pb, and Zn by the indicator plant Lupinus hartwegii and the Zn hyperaccumulator Thlaspi caerulescens (La Calamine ecotype) was a log-linear function of the in-situ measured HM soil solution concentrations. Over a wide range in dissolved Cd and Zn concentrations, uptake of these HMs by T. caerulescens was (much) greater than by L. hartwegii. Experimentally derived regression models describing the relationships between soil, soil solution, and plant were implemented in a HM mass balance model used to obtain estimates of the phytoextraction duration. For our target soils, estimates of the Cd phytoextraction duration using L. hartwegii or T. caerulescens increased significantly by more than 100 or 50 years when experimental soil—soil solution—plant relationships were used instead of the assumption of constant plant uptake of Cd. The two approaches gave similar results for phytoextraction of Zn by T. caerulescens.     

5-(2,6-difluorobenzyl)oxymethyl-5-methyl-3-(3-methylthiophen-2-yl)- 1,2-isoxazoline as a useful rice herbicide

5-(2,6-difluorobenzyl)oxymethyl-5-methyl-3-(3-methylthiophen-2-yl)-1,2-isoxazoline derivative was synthesized, and its herbicidal activity was assessed under glasshouse and flooded paddy conditions. 5-(2,6-Difluorobenzyl)oxymethyl-5-methyl-3-(3-methylthiophen-2-yl)-1,2-isoxazoline demonstrated good rice selectivity and potent herbicidal activity against annual weeds at 125 g of a.i. ha(-1) under greenhouse conditions. Soil application of this compound showed complete control of barnyard-grass to the fourth leaf stage at 250 g of a.i. ha(-1). Field trials indicated that this compound controlled annual weeds rapidly with a good tolerance on transplanted rice seedlings by post-emergence and soil application. This compound showed a low mammalian and environmental toxicity in various toxicological tests.     

农牧交错带牧业系统波动性及影响因子分析

牧业系统波动是影响到农牧交错带农户生计和生态安全的重大问题。该研究以羊只总数为牧业系统特征变量,选取年降水量、年平均气温、有效灌溉面积、粮食播种面积、粮食总产量、当年造林面积、草地面积、植被指数、农业劳动力人数和农民人均纯收入为影响因子。用经验模态分解法获得羊只总数及其10个影响因子的波动分量和趋势量;进而运用集对分析法对羊只总数与其影响因子的相应波动分量进行对比分析。结果表明,1954—2013年盐池县羊只总数波动较大,羊只总数及其10个影响因子存在3~4、7~15、22~26、40~45 a的准周期性波动,22~26 a准周期是羊只总数波动中最重要的。对羊只总数波动影响最大的因子是粮食播种面积,其次是年降水量,二者的综合影响率占75%。年平均气温、粮食总产量和造林面积也有一定影响。羊只总数波动分量的周期越长,受农业的影响越大;越短则受气候的影响越大。经济社会对羊只总数的波动影响很小。气候因子在3、7~9和30 a准周期上影响羊只总数波动,是其主控因子;而粮食总产量、当年造林面积是羊只总数波动的反向调节因子,其他6个因子兼有主控和反向调节两方面的作用。基于以上各主要影响因子的作用机理,构建有效的农林牧复合系统将是实现盐池县牧业系统稳定发展的必然出路。     

武汉天河机场T2航站楼中央空调冷却水系统整改方案

分析了武汉天河机场新建T2航站楼中央空调冷却水系统存在的问题,提出了解决问题的具体整改方案,整改结果达到了设备安全运行和节能减排的目的.     

5个香料烟品种3地2年的区域适应性研究

为了提高中国混合型卷烟的质量,选育适合不同生态区域种植的香料烟新品种.采用田间比较试验的方法,在保山市和德宏州的3个香料烟产区对5个香料烟品种进行了2年适应性研究,结果认为,对照品种'云香巴斯玛1号'表现稳定,应继续作为主栽品种;'保加利亚巴斯玛'和'巴斯玛14号'的品种特性、外观质量和烟叶产质量表现较好,可逐步扩大试...