全文获取类型
收费全文 | 268篇 |
免费 | 17篇 |
专业分类
林业 | 32篇 |
农学 | 11篇 |
基础科学 | 2篇 |
25篇 | |
综合类 | 24篇 |
农作物 | 49篇 |
水产渔业 | 10篇 |
畜牧兽医 | 119篇 |
园艺 | 7篇 |
植物保护 | 6篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 13篇 |
2020年 | 9篇 |
2019年 | 11篇 |
2018年 | 15篇 |
2017年 | 3篇 |
2016年 | 15篇 |
2015年 | 20篇 |
2014年 | 15篇 |
2013年 | 19篇 |
2012年 | 11篇 |
2011年 | 20篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 18篇 |
2007年 | 13篇 |
2006年 | 15篇 |
2005年 | 6篇 |
2004年 | 3篇 |
2003年 | 4篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 11篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1995年 | 5篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1991年 | 5篇 |
1990年 | 1篇 |
1984年 | 2篇 |
1979年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有285条查询结果,搜索用时 0 毫秒
251.
Min-Kyoung Shin Myung Hwan Jung Won-Jung Lee Pil Son Choi Yong-Suk Jang Han Sang Yoo 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):401-403
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection. 相似文献
252.
Gahyun Lee Seong Young Kwon Kyuyeol Son Seungjo Park Ju-hwan Lee Kyoung-Oh Cho Jung-Joon Min Jihye Choi 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):257-260
This report describes the usefulness of positron emission tomography-computed tomography (PET-CT) for evaluating recurrent or residual tumors following surgery. CT and 18F-fluorodeoxyglucose PET-CT were pre- and post-operatively applied to multiple masses in a dog with hemangiosarcoma. The distinction between the left subcutaneous mass and the peritoneum was clarified on pre-operative CT examination, and malignancy was suspected based on PET-CT. A recurrent or residual tumor in the left subcutaneous region was suspected on post-operative PET-CT, and confirmed through histopathologic examination. 相似文献
253.
SW PAGE 《Australian veterinary journal》1991,68(1):1-2
Chloramphenicol is a broad-spectrum antibiotic which has enjoyed extensive use in both medical and veterinary practice. Shortly after its introduction in the late 1940s, the use of chloramphenicol was associated with the induction of an idiosyncratic form of aplastic anaemia in man. This rare and unpredictable adverse effect has since been associated not only with systemic use but with topical applications, as well as occupational exposure. Recognition of the small risk of a potentially fatal adverse reaction, together with the risk of selection of chloramphenicol-resistant pathogens, has led to restrictions on the veterinary uses of chloramphenicol. In Australia at present, the use of chloramphenicol is only permitted in small animals. Its use is specifically prohibited in food-producing animals, including horses. 相似文献
254.
255.
Marsupostrongylus spp. are the metastrongyloid nematodes most commonly associated with verminous pneumonia in Australian marsupials. Currently, there is a scarcity of information regarding this parasite in the common brushtail possum (Trichosurus vulpecula). Thirty-four free-living possums submitted to two wildlife hospitals in Sydney, Australia, between 2008 and 2015 were diagnosed with verminous pneumonia on postmortem examination. The majority of possums presented ill with multiple comorbidities. However, only five cases had clinical signs of respiratory disease. Necropsy and histopathology revealed extensive lung lesions characterised by diffuse, mixed interstitial infiltrates of macrophages, lymphocytes and plasma cells with mild to marked concentrations of eosinophils. Bronchopneumonia, pulmonary oedema, interstitial fibrosis, atelectasis and type II pneumocyte hyperplasia were also present in most cases. Adult nematodes, first-stage larvae and embryonating eggs were present in the large airways and alveolar spaces. The parasites were definitively identified as Marsupostrongylus spp. in eight cases with presumptive diagnoses based on histopathological characteristics reached in a further 26 cases. Twenty-nine of the 34 affected possums were adults with no sex predisposition. A review of the brushtail possum records at Taronga Wildlife Hospital from 1999 to 2015 revealed no lungworm infections were reported in the 45 possums examined before 2008. However, between 2008 and 2015, 30 of 47 possums (63.8%) examined were diagnosed with metastrongyloid lungworms. This case series is the first detailed report of Marsupostrongylus nematodes in common brushtail possums and highlights the clinical and pathological features, along with epidemiological findings. 相似文献
256.
Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low fertilization ability in Ban—A native Vietnamese pigs 下载免费PDF全文
Nguyen Viet Linh Tamas Somfai Thi Hiep Nguyen Nguyen Thi Nhung Nguyen Thi Hong Nguyen Tien Dat Nguyen Hoang Thinh Nguyen Khanh Van Dong Van Quyen Hoang Ha Chu Nguyen Thanh Son Kazuhiro Kikuchi 《Animal Science Journal》2018,89(8):1079-1084
The aim of the present study was to improve the penetration during in vitro fertilization (IVF) of a frozen lot of epididymal sperm with a notoriously low fertilization ability of a Ban boar which is a native Vietnamese breed by optimizing different parameters of the IVF system. In Experiment 1, we determined that Pig‐fertilization medium was superior medium to Tyrode's albumin lactate pyruvate‐polyvinyl alcohol medium for IVF and defined the optimum the sperm concentration (1 × 106 sperm/ml). In Experiment 2, we clarified that partial removal of cumulus cells from cumulus‐oocyte complexes by hyaluronidase treatment before IVF enhances sperm penetration, whereas complete cumulus removal reduces penetration. Finally, in Experiment 3 the elevation of concentration of caffeine in Pig‐fertilization medium from 2 to 5 mmol/L and the prolongation of the co‐culture of gametes from 3 to 5 hr significantly increased the total penetration rate from 15.2% to over 50%. In conclusion, the combination of partial oocyte denudation, an elevated caffeine concentration in Pig‐fertilization medium and an extended interval of IVF with using an optimized sperm concentration was a potent way to improve the fertilization results for a frozen epididymal Ban sperm lot with low fertility. 相似文献
257.
Won-gyun Son Min Jang Sang-min Jo Hyunseok Kim Chi Won Shin Inhyung Lee 《Veterinary anaesthesia and analgesia》2019,46(1):96-105
Objective
To compare the effects of epidural injection of three volumes of lidocaine injected at the third (T3) or eleventh thoracic vertebra (T11) in conscious dogs to induce thoracic epidural anesthesia (TEA) and to measure the epidural dispersion of iohexol under similar conditions.Study design
Prospective crossover experiment.Animals
A group of five Beagle dogs weighing 10.4 ± 0.5 kg (mean ± standard deviation).Methods
Each dog was anesthetized twice, separated by 1 week, for inserting an epidural catheter at the lumbosacral space and advancing the tip to T3 (treatment TEAT3) or T11 (treatment TEAT11). For each treatment, three volumes of 2% lidocaine (0.05, 0.10 and 0.20 mL kg–1) were administered at 24 hour intervals, and sensory blockade (SB) of dermatomes was estimated by pinching the skin with mosquito forceps. Under identical conditions of injection volume and site, iohexol was administered 3 hours after lidocaine injection to identify epidural distribution (ED) using computed tomography. The effects of injection site and volume on SB of thoracic dermatomes and ED were analyzed using a linear mixed model (p < 0.05).Results
Thoracic SB and ED significantly increased as the volume increased (p < 0.001 and p < 0.001, respectively), and significantly decreased in TEAT3 than in TEAT11 (p = 0.011 and p = 0.002, respectively). Cervical SB was obtained in three of five dogs in TEAT3 and two of five dogs in TEAT11 injected with 0.20 mL kg–1. One dog showed temporary inspiratory stridor probably caused by bilateral laryngeal paralysis, but no hypoxia.Conclusions and clinical relevance
TEA induced at T3 produced less thoracic SB than did TEA at T11 with the same volumes of lidocaine. The cervical SB obtained with the highest volume of lidocaine may increase the risk of laryngeal paralysis and pulmonary aspiration. 相似文献258.
A novel balloon technique to induce intra‐abdominal hypertension and its effects on cardiovascular parameters in a conscious dog model 下载免费PDF全文
259.
Nguyen V. Son Kazuyuki Uchida Atigan Thongtharb James K. Chambers Takuya E. Kishimoto Hirotaka Tomiyasu Aki Ohmi Hajime Tsujimoto Hiroyuki Nakayama 《Veterinary and comparative oncology》2019,17(3):345-353
A cell line named FB‐LCH01, derived from a dog diagnosed with Langerhans cell histiocytosis (LCH), was established and characterized. FB‐LCH01 had C‐shaped nucleoli, characterized by modal chromosome aberrations. The original tumour cells as well as established FB‐LCH01 cells were immunopositive for human leukocyte antigen‐DR, Iba‐1 and E‐cadherin, and immunonegative for CD163 and CD204, suggesting Langerhans cell origin. Furthermore, the characteristics of FB‐LCH01 were compared with those of two canine histiocytic sarcoma cell lines (PWC‐HS01 and FCR‐HS02) established previously. Expression of E‐cadherin was detected only in FB‐LCH01, but not in PWC‐HS01 and FCR‐HS02. All (n = 9) the severe combined immunodeficiency mice inoculated with the FB‐LCH01 cells developed subcutaneous tumour masses after 3 weeks. Eight of nine mice also developed metastatic lesions in the lymph nodes (8/8; 100%), lung (5/8; 62.5%), stomach (5/8; 62.5%), heart (4/8; 50%), pancreas (4/8; 50%), kidney (3/8; 37.5%), skin (3/8; 37.5%) and bone marrow (1/8; 12.5%). Tumour cells were pleomorphic and round‐ to polygonal‐shaped with prominent anisocytosis and anisokaryosis. The xenotransplanted tumour cells maintained the immunohistochemical features of the original tumour with persistent E‐cadherin expression at injection site and some visceral organs. In conclusion, the established cell line as well as the mice xenotransplant model in this study reflect the nature of canine LCH and may serve as promising models for investigating the patho‐tumorigenesis and therapy of the disease. 相似文献
260.
松墨天牛是松树的主要蛀干害虫,亦是松材线虫病的主要传播媒介。利用引诱剂能够大量诱集松墨天牛,使用浓度以1:3较为经济实用。1号引诱剂诱虫能力最强,1、2号混合液及4号诱虫效果次之。诱虫量一般比对照提高17.8~54.9倍,比伐倒木提高1.2~3.2倍,比病虫枯死木提高5.8~7.2倍。诱集后可降低林木枯死率2%~32%,防治效果达70%~95%。施引诱剂的同时再施1:1的氧化乐果稀释液可毒杀62.4%~100%的天牛幼虫。每公顷释放管氏肿腿蜂28 142头,当代防治效果可达40%~50%,经3~4个月持续防治效果可达69.6%~85.3%。 相似文献