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61.
62.
Scrapie associated fibrils in the diagnosis of scrapie in sheep 总被引:2,自引:0,他引:2
P H Gibson R A Somerville H Fraser J D Foster R H Kimberlin 《The Veterinary record》1987,120(6):125-127
Previous research has consistently demonstrated by electron microscopy the presence of scrapie associated fibrils in brain extracts prepared from mice and hamsters with clinical signs of experimental scrapie. In the present study similar fibrils were seen in all the brain extracts prepared from 11 Cheviot or Suffolk sheep with natural or experimental scrapie that had been diagnosed clinically and confirmed neuropathologically. They were not found in the brain extracts of nine sheep that did not have scrapie and which included four that had been injected with infected material but did not develop the disease. The presence of such fibrils can therefore be used as an additional diagnostic criterion for natural scrapie in sheep. 相似文献
63.
Toward a systems approach to understanding plant cell walls 总被引:2,自引:0,他引:2
Somerville C Bauer S Brininstool G Facette M Hamann T Milne J Osborne E Paredez A Persson S Raab T Vorwerk S Youngs H 《Science (New York, N.Y.)》2004,306(5705):2206-2211
One of the defining features of plants is a body plan based on the physical properties of cell walls. Structural analyses of the polysaccharide components, combined with high-resolution imaging, have provided the basis for much of the current understanding of cell walls. The application of genetic methods has begun to provide new insights into how walls are made, how they are controlled, and how they function. However, progress in integrating biophysical, developmental, and genetic information into a useful model will require a system-based approach. 相似文献
64.
Nishimura MT Stein M Hou BH Vogel JP Edwards H Somerville SC 《Science (New York, N.Y.)》2003,301(5635):969-972
Plants attacked by pathogens rapidly deposit callose, a beta-1,3-glucan, at wound sites. Traditionally, this deposition is thought to reinforce the cell wall and is regarded as a defense response. Surprisingly, here we found that powdery mildew resistant 4 (pmr4), a mutant lacking pathogen-induced callose, became resistant to pathogens, rather than more susceptible. This resistance was due to mutation of a callose synthase, resulting in a loss of the induced callose response. Double-mutant analysis indicated that blocking the salicylic acid (SA) defense signaling pathway was sufficient to restore susceptibility to pmr4 mutants. Thus, callose or callose synthase negatively regulates the SA pathway. 相似文献
65.
66.
Coprological examination was used to determine prevalence of gastrointestinal helminthes in a sample of 231 dogs (117 females and 114 males) during the summer of 2009 at a veterinary clinic in south central West Virginia, USA. Clinical signs (e.g., diarrhea, vomiting, weight gain or loss) were noted in addition to a history of anthelmintic usage. A total of 79 dogs (33.6%) were infected with one or more intestinal nematodes. Most dogs (58) were parasitized with a single species, 19 were parasitized with 2 species, and 2 were parasitized by 3 species. There was no significant difference (i.e., X(2)<3.84; P>0.05) in prevalence of infection between female and male dogs for any of the identified nematode species. The chi-square test for equality of proportions was used to determine prevalence of infection in 3 age categories of dogs (females and males combined): young dogs (≤12 months of age); mature dogs (13-83 months); and old dogs >83 months. Prevalences of infection for Ancylostoma caninum and Toxocara canis were significantly (P<0.005) higher in young dogs, whereas there was no significant difference (P>0.05) in prevalence by age category for Trichuris vulpis. Dogs exhibiting clinical signs were no more likely to harbor intestinal nematodes than dogs that were asymptomatic. Additionally, dogs receiving heartworm treatment were significantly less likely to be parasitized than dogs receiving no heartworm prophylaxis. 相似文献
67.
Ethylene influences a number of developmental processes and responses to stress in higher plants. The molecular basis for the action of ethylene was investigated in mutants of Arabidopsis thaliana that have altered responses to ethylene. One mutant line, which has a dominant mutation at a locus designated etr, lacks a number of responses to ethylene that are present in the wild-type plant. These include inhibition of cell elongation, promotion of seed germination, enhancement of peroxidase activity, acceleration of leaf senescence, and feedback suppression of ethylene synthesis by ethylene. These diverse responses, which occur in different tissues of Arabidopsis, appear to share some common element in their transduction pathways-for example, a single receptor for ethylene. Results of ethylene binding experiments in vivo indicate that this receptor may be affected by the etr mutation. 相似文献
68.
Infection-specific particle from the unconventional slow virus diseases 总被引:27,自引:0,他引:27
P A Merz R G Rohwer R Kascsak H M Wisniewski R A Somerville C J Gibbs D C Gajdusek 《Science (New York, N.Y.)》1984,225(4660):437-440
Scrapie-associated fibrils, first observed in brains of scrapie-infected mice, were also observed in scrapie-infected hamsters and monkeys, in humans with Creutzfeldt-Jakob disease, and in kuru-infected monkeys. These fibrils were not found in a comprehensive series of control brains from humans and animals affected with central nervous system disorders resulting in histopathologies, ultrastructural features, or disease symptoms similar to those of scrapie, kuru, and Creutzfeldt-Jakob disease. These fibrils are also found in preclinical scrapie and in the spleens of scrapie-infected mice; they are a specific marker for the "unconventional" slow virus diseases, and may be the etiological agent. 相似文献
69.
70.
The cape mendocino, california, earthquakes of april 1992: subduction at the triple junction 总被引:1,自引:0,他引:1
Oppenheimer D Eaton J Jayko A Lisowski M Marshall G Murray M Simpson R Stein R Beroza G Magee M Carver G Dengler L McPherson R Gee L Romanowicz B Gonzalez F Li WH Satake K Somerville P Valentine D 《Science (New York, N.Y.)》1993,261(5120):433-438
The 25 April 1992 magnitude 7.1 Cape Mendocino thrust earthquake demonstrated that the North America-Gorda plate boundary is seismogenic and illustrated hazards that could result from much larger earthquakes forecast for the Cascadia region. The shock occurred just north of the Mendocino Triple Junction and caused strong ground motion and moderate damage in the immediate area. Rupture initiated onshore at a depth of 10.5 kilometers and propagated up-dip and seaward. Slip on steep faults in the Gorda plate generated two magnitude 6.6 aftershocks on 26 April. The main shock did not produce surface rupture on land but caused coastal uplift and a tsunami. The emerging picture of seismicity and faulting at the triple junction suggests that the region is likely to continue experiencing significant seismicity. 相似文献