Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis

Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.     

Induction in vitro of antibodies to phage T2: antigens in the RNA extract employed

Cells from rat peritoneal exudate incubated in vitro with phage T2 yield RNA which induces cultures of rat lymph nodes to form antibodies. Complement fixation reveals T2 head, tail, and internal protein antigens in the RNA. Inoculating the RNA into mice that had previously been stimulated primarily with whole phage T2 results in a secondary response of neutralizing antibody.     

Ancient geodynamics and global-scale hydrology on Mars

Loading of the lithosphere of Mars by the Tharsis rise explains much of the global shape and long-wavelength gravity field of the planet, including a ring of negative gravity anomalies and a topographic trough around Tharsis, as well as gravity anomaly and topographic highs centered in Arabia Terra and extending northward toward Utopia. The Tharsis-induced trough and antipodal high were largely in place by the end of the Noachian Epoch and exerted control on the location and orientation of valley networks. The release of carbon dioxide and water accompanying the emplacement of approximately 3 x 10(8) cubic kilometers of Tharsis magmas may have sustained a warmer climate than at present, enabling the formation of ancient valley networks and fluvial landscape denudation in and adjacent to the large-scale trough.     

Global variations in the geoid/topography admittance of venus

Global representations of geoid height and topography are used to map variations in the geoid/topography ratio (admittance) of Venus. The admittance values are permissive of two mutually exclusive models for convection-driven topography. In the first, compressive highland plateaus are expressions of present mantle downwelling, broad volcanic rises are expressions of mantle upwelling, and lowlands overlie regions with no substantial vertical motion in the mantle. In the second, compressive highland plateaus are remnants of an earlier regime of high crustal strain, and most other long-wavelength topographic variations arise from normal convective tractions at the base of the lithosphere.