Modeling the relations of thyroid hormones in rats

The relations between the concentration of thyroxine, triiodothyronine, and thyrotropic hormone in the peripheral blood of white rats are studied in an experiment with the administration of a thyrostatic drug (merkazolil) in an optimally allowable daily dose. A high reliability of the mathematical model developed for reproducing the level of the content of hormones of the hypophysial-thyroid system is established.     

Orobanche palaestina: a potential threat to agricultural crops in Israel

Weeds of the genus Orobanche are obligatory chlorophyll-lacking root parasites that infect and severely damage many dicotyledonous agricultural crops in warm-temperate and subtropical regions of the world. The genus comprises over 100 species, at least six of which are notable weeds. Orobanche palaestina Reut. is an endemic Mediterranean species, previously reported to parasitize thistles [Notobasis syriaca (L.) Cass. and Cirsium phyllocephalum Boiss. & Bl.] and annual legumes in native Mediterranean habitats, mainly in disturbed places such as roadsides and waste sites. In recent years, we have observed a rapid expansion of O. palaestina populations adjacent to commercial agricultural fields in the Jezreel Valley of Israel. The ability of O. palaestina to infect crops was tested in pots under nethouse conditions. The experiments revealed that O. palaestina is able to parasitize agricultural crops. Safflower (Carthamus tinctorius L.), lettuce (Lactuca sativa L.), gazania (Gazania uniflora Gaertn.), vetch (Vicia sativa L.) and artichoke (Cynara scolymus L.) were found to be potential hosts for this parasite, and all of them, except vetch and artichoke, were preferred to thistle, the native host. Sunflower (Helianthus annuus L.) induced O. palaestina seed germination and root attachment, but the attached parasites could not reach maturity and died soon after the attachment stage. Tomato (Lycopersicon esculentum Mill.), carrot (Daucus carota L.), garland chrysanthemum (Chrysanthemum coronarium L.) and cabbage (Brassica oleraceae L.) were not parasitized by O. palaestina.     

Formation of the hypersensitivity response due to the expression of <Emphasis Type="Italic">FeSOD1</Emphasis> gene in tomato when it is inoculated with <Emphasis Type="Italic">Phytophthora infestans</Emphasis>

The activity of antioxidant enzymes and ultrastructural changes in tissues inoculated with P. infestans isolate have been studied in the previously developed independent transgenic lines of tomato with FeSOD1 gene and control plants. It is shown that the activity of superoxide dismutase is significantly higher in transgenic plants than that in control plants (nontransgenic plants). Chlorosis and obvious changes in tissue turgor were observed when the control tomato plants were inoculated, which indicates irreversible damages and unimpeded progression of infection. At the same time, the transgenic lines were characterized by the formation of clearly limited zones of damaged cells that rapidly arrested the infection. In addition, the damages differed from those in nontransgenic plants: the cells along the edges of the infection site were smaller and had heavy invaginations of the cell wall. The contacts between the cells were disrupted in this zone, but they were preserved in undisturbed zones of the tissue. Thus, the expression of the FeSOD1 transgene promotes the emergence of the resistance to P. infestans in tomato transgenic plants.     

Cyperus rotundus control using a mechanical digger and solar radiation

Cyperus rotundus (purple nutsedge) is considered one of the most noxious weeds affecting agricultural areas worldwide. With its fast growth rate, it competes with annual crops for water, minerals, light and space. It excretes allelopathic materials that impede crop development. Controlling this weed is difficult and is done mostly by manual weeding, cultivation and herbicides, with limited effectiveness. A method was developed for the control of C. rotundus. A machine penetrates the soil and rearranges it, so that the tubers are lifted to the upper soil layer, where they are left exposed to the hot summer climate, dehydrate and die. The method was tested in seven field experiments on various soil types. Two months after the experimental plots were irrigated, 70–100% weed control was observed. The machine's speed (at 1.2 or 1.8 km h^?1) and the number of treatments (one, or two treatments a month apart) did not influence the level of weed control. The method has a very high potential to replace manual weeding and application of herbicides. Further work will test whether the treatment has a long‐term effect or should be repeated every season.     

Expression of genes encoding chitin-binding proteins (PR-4) and hevein-like antimicrobial peptides in transgenic tomato plants enhanced resistanse to <Emphasis Type="Italic">Phytophthora infestance</Emphasis>

The resistance of transgenic tomato plants containing genes of chitin-binding proteins from Amaranthus caudatus (ac) and A. retraflexus (RS-intron-Shir) and hevein-like antimicrobial peptides (amp1 and amp2) from Stellaria media is analyzed. Lines expressing genes ac, amp1, and amp2 are characterized, unlike the untransformed control, by enhanced resistance to a isolate Phytophthora infestans obtained from potato leaves.     

Carbon Stock in Sandy and Loamy Soils of Coniferous–Broadleaved Forests at Different Succession Stages

Eurasian Soil Science - A comparative assessment of the carbon stock in loamy soils (Albic Retisols) of coniferous–broadleaved forests on the Moskvoretsko-Oksky Plain and sandy soils (Albic...     

Succession of Microbial Community in Gray Forest Soil during the Decomposition of Different Organic Compounds

Eurasian Soil Science - In a model laboratory experiment on gray forest soil, the succession of microbial communities during the decomposition of proteins, lipids, and polysaccharides was studied....     

Snake envenomation in cats and its detection by rapid immunoassay

Objective To determine the usefulness of a snake venom detection kit (SVDK) in the management of envenomed cats.
Design A clinical study.  

Animals

Twenty-two cats were investigated.
Procedure Cats injected subcutaneously with approximately 0.25 or 1.0 lethal dose (LD) of tiger snake venom or 1 or 4 LD of brown snake venom were observed for clinical symptoms of envenomation at intervals over the ensuring 24 to 48 hours(h). Blood and urine samples were taken at regular intervals and assayed in a quantitative laboratory assay for snake venoms. Selected samples were assayed in parallel in a rapid, semi-quantitative SVDK.
Results The studies showed that it was important to estimate the elapsed time from envenomation to presentation. If this time was less than 8 h, blood was the most appropriate sample and a negative result should exclude serious envenomation. If the elapsed time exceeded 8 h, it was essential that urine be sampled. Venom levels in urine were high at 8 h and approached the level of test sensitivity over 24 to 48 h; however by this time clinical signs were obvious in endangered cats.  

Conclusions

Careful use of the SVDK is a valuable aid in the management of a potentially envenomed cat.