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991.
MK Singh KP Singh D Kumar RA Shah T Anand MS Chauhan RS Manik SK Singla P Palta 《Reproduction in domestic animals》2013,48(2):284-291
When buffalo embryonic stem (ES) cell–like cells that expressed surface markers SSEA‐4, TRA‐1‐60, TRA‐1‐81, CD9 and CD90 and intracellular markers OCT4, SOX2 and FOXD3, as shown by immunofluorescence, and that expressed REX‐1 and NUCLEOSTEMIN as confirmed by RT‐PCR, were subjected to suspension culture in hanging drops in absence of LIF and buffalo foetal fibroblast feeder layer support, they differentiated to form three‐dimensional embryoid bodies (EBs). Of 231 EBs examined on Day 3 of suspension culture, 141 (61.3 ± 3.09%) were of compact type, whereas 90 (38.4 ± 3.12%) were of cystic type. The cells obtained from EBs were found to express NF‐68 and NESTIN (ectodermal lineage), BMP‐4 and α‐skeletal actin (mesodermal lineage), and α‐fetoprotein, GATA‐4 and HNF‐4 (endodermal lineage). When these EBs were cultured on gelatin‐coated dishes, they spontaneously differentiated to several cell types such as epithelial‐ and neuron‐like cells. When EBs were cultured in the presence of 1 or 2% DMSO or 10?8 m or 10?7 m retinoic acid for 25 days, ES cells could be directed to form muscle cell–like cells, the identity of which was confirmed by expression of α‐actinin by immunofluorescence and of MYF‐5, MYOD and MYOGENIN genes by RT‐PCR. MYOD was first detected on Day 10 in both treatment groups and on Day 15 in controls, whereas MYOGENIN was first detected on Day 10, Day 15 and Day 25 in the presence of retinoic acid, in the presence of DMSO and in controls, respectively. The present study demonstrates the ability of buffalo ES cell–like cells to undergo directed differentiation to cells of skeletal myogenic lineage. 相似文献
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Audrey C. Muguet‐Chanoit DVM MS Ji‐Hey Lim DVM PhD Ryan Gallagher DVM Zachary Niman DVM Diplomate ACVIM Neurology Stacy Dillard DVM Diplomate ACVIM Neurology James Campbell DVM PhD Peter Early DVM Diplomate ACVIM Neurology Karen R. Muñana DVM MS Diplomate ACVIM Neurology Courtenay Freeman DVM Marc Kent DVM Diplomate ACVIM Neurology Carley Giovanella DVM Diplomate ACVIM Neurology 《Veterinary surgery : VS》2012,41(2):200-206
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Julia P. Sumner BVSc MACVSc Diplomate ACVS Robert J. Hardie DVM Diplomate ACVS Diplomate ECVS Jamie N. Henningson DVM PhD Diplomate ACVP Randi Drees DMV Diplomate ACVR Diplomate ECVDI Mark D. Markel DVM PhD Diplomate ACVS Dale Bjorling DVM MS Diplomate ACVS 《Veterinary surgery : VS》2012,41(6):655-663
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