仔猪黄痢的鉴别诊断与防治

仔猪黄痢又称为早发性大肠杆菌病,由致病性埃希氏大肠杆菌所引起.是出生仔猪的急性致死性肠道传染病,以下痢、脱水、排黄色或黄白色粪便为特征.     

茄子新品种吉茄6号的选育

吉茄6号是以高代自交系B4-3为母本,以C2-6为父本配制而成的茄子一代杂种。早熟,植株生长势较强,始花节位为第9~11节,果实中长棒状,纵径24~28cm,横径5cm左右,单果质量110~150g。果皮黑紫色,有光泽,肉质细嫩,坐果率高。耐低温、耐弱光性强,对茄子黄萎病抗性较强。每667m~2产量3100kg左右。适合吉林省各地栽培。     

新疆典型膜下滴灌棉花种植模式的用水效率与效益

膜下滴灌技术因具有显著的节水、保温、抑盐、增产效果,在新疆自治区棉田中已获得大面积推广应用。在目前的大田棉花生产条件下,结合当地的光热、土壤、机械等条件,因地制宜选择合理的膜下滴灌种植模式,对合理调控棉田土壤水盐分布、促进棉花生长与增产、提高劳动生产率和增加棉农收入等具有十分重要的意义。迄今为止,在新疆自治区主要存在3种典型的膜下滴灌棉花种植模式,分别为传统模式、机采模式和超宽膜模式。该文通过开展实地调查取样,基于种植密度、灌溉定额、根区土壤水盐分布、覆膜宽度以及其它影响棉花生产收益的因素,对3种典型膜下滴灌棉花种植模式的水分利用效率与经济效益进行了对比分析。结果表明,由于各种种植模式下不同的种植密度、灌溉定额、根区土壤水盐分布、覆膜宽度等对棉花的耗水与产量产生了较大影响,导致棉花的水分利用效率存在明显差异：超宽膜模式棉花的水分利用效率为1.04 kg/m3,明显高于传统模式的0.98 kg/m3与机采模式的0.89 kg/m3。另外,经济效益受种植模式（影响前期投入与棉花产量）与采棉方式（影响采棉支出与籽棉收购单价）的影响较大：超宽膜模式具有最高的经济效益,其单位面积纯收入达18582元/hm2,稍高于机采模式下的18298元/hm2,传统模式下纯收入最低,仅11725元/hm2。因此,为高效利用农业水资源并增加种棉收益,建议在新疆自治区大力推广超宽膜模式,并对现有采棉机进行适当改进以在超宽膜模式下实现采棉机械化;或适当调整现有机采模式下的滴灌带布置形式（如将其布置在窄行的中央）,但相关的效应仍有待更进一步研究。     

Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in Arabidopsis

Stabilization of the levels of active oxygen species (AOS) is important to the survival of organisms. To clarify the system controlling levels of AOS in plants, this study used an electron spin resonance (ESR) method to directly measure superoxide radical (O(2)(.-)) scavenging activities in the wild-type Arabidopsis thaliana (Col and Ler ecotypes), two anthocyanin mutants (tt3 and ttg1), and an ascorbic acid mutant (vtc1). Under ordinary growth conditions, Arabidopsis contained superoxide-scavenging activity (SOSA) of approximately 300-500 SOD units/g of fresh weight. The ESR pattern indicated that most (40-50%) of this activity was due to ascorbic acid. For the analysis of SOSA under conditions of oxidative stress, synthesis of AOS was induced by gamma-irradiation. The radical scavenging activity in irradiated plants increased approximately 10-fold following an associated increase in the accumulation of ascorbic acid and anthocyanin. The accumulation of ascorbic acid and anthocyanin was suppressed by treatment with an antioxidant before irradiation and was induced by treatment with a radical-generating reagent. The contributions of ascorbic acid and anthocyanin to the total superoxide radical scavenging activity differed among ecotypes. In the Ler ecotype, ascorbic acid accumulated at twice the level of that in the Col ecotype, and induction of anthocyanin was half that in Col. To confirm the activity of ascorbic acid and anthocyanin against AOS stress, the viability of the wild type and mutants (tt2, tt3,tt5, ttg1, and vtc1) was examined after gamma-irradiation. Only the plants in which ascorbic acid and anthocyanin were induced had the ability to grow and flower.     

中国区域畜禽粪便能源潜力及总量控制研究

为了评估中国畜禽粪便资源总量及其对环境的影响,以环保部、统计局和农业部发布的区域畜禽产排污系数为基础,利用2010年的统计数据,研究了中国及各省的畜禽粪便资源总量、能源潜力及农地的氮磷负荷,并以欧盟的农地氮磷施用标准对中国畜禽养殖的环境容量和污染风险进行了初步评估。结果表明,2010年,中国畜禽粪便总量达22.35亿t,可产沼气1072.75亿m3,山东等6省市粪便资源超过1.00亿t;全国单位面积农地氮磷平均负荷为43.73kg/hm2(TN)和9.16kg/hm2(TP),北京等6省市农地氮磷负荷超标;全国畜禽养殖环境容量为129.56亿头猪当量(以N为基准),159.74亿头猪当量(以P为基准),实际养殖总量约占环境容量的1/4,考虑化肥施用的影响,约有20个省超过本省50%环境容量。研究结果为区域畜禽养殖总量控制、合理布局和粪污的综合利用提供决策依据。     

苹果开心形树冠不同部位光合与蒸腾能力的研究

 以18年生开心形富士苹果(Malus pumila Mill. ‘Red Fuji’) 为试材, 定点测定了冠层不同部位晴天时的光照分布、气孔导度、光合速率和蒸腾速率。结果表明, 树冠外围的光照强度、单叶光合能力比较高, 内膛、中部和外围叶片最大光合速率分别为13.46、14.69和15.98μmol·m^{- 2} ·s^{- 1}。气孔导度、光合速率和水分利用效率的日变化呈双峰曲线, 而蒸腾速率呈单峰曲线, 在中午外围叶片的蒸腾速率略有降低。内膛、中部和外围叶片在晴天的平均光合速率分别为4.53、6.63和6.54μmol·m^{- 2} ·s^{- 1} , 平均蒸腾速率分别为3.36、4.06和4.40μmol·m^{- 2} ·s^{- 1}。这说明苹果在冠层不同部位的光合与蒸腾能力存在一定的差别, 冠层中部的光合速率和水分利用效率最大, 外围的蒸腾能力最强, 内膛最差。     

螺旋输送与筛筒组合式分离装置性能试验

大中型谷物联合收割机分离装置存在体积庞大、振动强等问题,小型谷物联合收割机分离装置缺乏对断茎秆的处理能力且分离能力较弱。为此,提出了一种螺旋输送与筛筒组合式分离装置。通过室内试验,研究了搅龙转速、装置倾角和分离筛孔尺寸对分离性能的影响,得出了最优结构和运动参数组合,实现了籽粒、颖糠及茎秆在输送过程中的有效分离,为清选装置在谷物联合收割机上的应用提供了依据。     

24％甲萘威·吡蚜酮可湿性粉剂对稻飞虱的控制作用

2009—2010年在广州番禺进行24%甲萘威·吡蚜酮可湿性粉剂防治水稻稻飞虱田间药效试验,结果表明,24%甲萘威·吡蚜酮可湿性粉剂制剂每667 m2用量110、140、170 g处理对水稻稻飞虱速效性差,但持效期长,具有较好的防治效果;药后1、3、7 d,3个剂量处理对稻飞虱连续两年的防治效果分别为64%～71%、75%～84%和80%～95%,能有效控制稻飞虱的危害。生产上,推荐24%甲萘威·吡蚜酮可湿性粉剂防治稻飞虱的制剂用量为每667 m2用110～140 g。     

Black soybean promotes the formation of active components with antihepatoma activity in the fermentation product of Agaricus blazei

The antihepatoma activity and related active components in the fermentation products of Agaricus blazei (AB) cultured in the medium containing soybean (S) or black soybean (BS) were investigated. AB(BS)-pE and AB(S)-pE were the ethanolic extracts from the fermentation products of AB(BS) and AB(S), respectively. According to the IC 50 values, AB(BS)-pE (161.1 and 24.0 microg/mL for Hep 3B and Hep G2 cells, respectively) exhibited stronger cytotoxicities against hepatoma cells than AB(S)-pE (>200 and 99.9 microg/mL for Hep 3B and Hep G2 cells, respectively). AB(BS)-pE was separated by silica gel column chromatography and eluted with n-hexane/ethyl acetate/methanol gradient solvent system into 21 fractions. Fraction 3 [AB(BS)-pE-F3], eluted with n-hexane/ethyl acetate (97:3 and 19:1, v/v), was the most active fraction having inhibitory activity on the proliferation of Hep 3B and Hep G2 cells (IC 50 of 3.6 and 1.9 microg/mL, respectively). Three major compounds, compounds 1- 3, were further isolated from the AB(BS)-pE-F3 fraction by reversed-phase semipreparative high-performance liquid chromatography. Compounds 2 and 3 gave better antihepatoma activity than that of compound 1. The IC 50 values of compounds 2 and 3 were 2.8 and 4.5 microg/mL for Hep 3B cells and 1.4 and 2.0 microg/mL for Hep G2 cells, respectively. The structures of compounds 2 and 3 were identified by UV, IR, electron impact mass spectrometry, and (1)H and (13)C NMR to be blazeispirols A and C, respectively. Blazeispirols A and C existed in the mycelia but not in the broth and were more in AB(BS)-pE (49.9 +/- 8.9 and 14.2 +/- 2.4 mg/g, respectively) than AB(S)-pE (15.9 +/- 1.7 and 3.9 +/- 0.6 mg/g, respectively). Additionally, the result shows that the production of blazeispirols A and C was increased after cultivation in the medium containing black soybean on day 6 and reached the maximum on day 12, and the contents of blazeispirols A and C were negatively correlated with Hep 3B and Hep G2 cell viabilities ( r = -0.84 to -0.93, P < 0.01). It suggests that blazeispirols A and C could be used as biomarkers to produce the fermentation product of A. blazei with antihepatoma activity.     

小麦叶片光呼吸与蛋白质积累关系的研究

以蛋白质含量较高的小麦品种ＰＨ８２－２－２、蛋白质含量较低的小麦品种泰山１号为试验材料,测定ＮａＨＳＯ３处理后小麦叶片的光合速率、光呼吸、小麦叶片硝酸还原酶活性、游离氨基酸含量以及籽粒蛋白质含量。结果表明,蛋白质含量较高的小麦叶片光呼吸高于蛋白质含量较低的小麦品种叶片光呼吸,而且不管光呼吸抑制与否均有此规律。小麦叶片光呼吸与蛋白质积累有密切的关系。