蚕蛹蛋白提取研究

以蚕蛹为原料,石油醚为溶剂进行脱脂,采用碱法提取蚕蛹蛋白,并对脱脂条件进行了研究。结果表明,以石油醚为脱脂溶剂,其脱脂条件为固液比1:4,温度50℃,时间4 h。重复4次,脱脂率达到96%以上。再以双氧水进行脱色、除臭,可以得到黄色无臭味蚕蛹蛋白粉,其蛋白含量为83.5%,蛋白质回收率为32.59%。     

提高食品微生物学实验课教学质量的探索

从采用灵活多变的教学手段、自行设计实验内容、强化实验操作技能和养成实验操作习惯等方面,提出如何提高食品微生物学实验课的教学质量,培养学生动手能力、独立思考解决问题的能力、创新能力。     

魔芋再制奶酪的研制

以天然切达奶酪和魔芋粉为主要原料,研究了魔芋再制奶酪的生产工艺,采用L9(34)正交试验法,分析了不同切达奶酪添加比例、魔芋粉的添加量、乳化盐添加量和乳化时间对魔芋再制奶酪色泽、滋气味和组织状态的影响,确定了生产魔芋再制奶酪的最佳工艺条件为:不同切达奶酪(3个月成熟∶9个月成熟)添加比例1∶3,质量分数6%的魔芋粉,质量分数2%的乳化盐,乳化时间6 min。由此制得的魔芋再制干酪,不仅可以保持传统再制干酪的组织状态,并且具有魔芋的滋气味。     

黄曲霉毒素的检测技术及预防措施

黄曲霉毒素是危害较大的毒素之一,给食品、饲料和畜牧等行业造成了极大的损失。由于它的强致病性及强致癌性,很早就受到了广大科学家及国际粮农组织的广泛关注。针对黄曲霉毒素的众多检测技术进行了归纳和总结,并比较了优缺点,为更好的检测黄曲霉毒素提供了理论依据。针对黄曲霉毒素的高度毒性,提出防治措施,以期保护公众健康、减少经济损失。     

农杆菌介导的地被菊遗传转化体系的优化

本研究以地被菊‘北林黄’无菌苗叶片外植体为受体材料,以卡那霉素抗性基因为选择标记基因,对农杆菌介导的地被菊‘北林黄’的遗传转化体系进行优化。研究结果表明:选取植株中部叶片为转化受体,外植体在经过1d的预培养,用活化的OD600为0.6的农杆菌侵染10min,共培养2d,再经过3d延迟培养后转移到筛选培养基上培养,有利于提高遗传转化效率,外植体的愈伤组织获得数和抗性芽数均最高。在筛选后期,不定芽生根阶段使用的Kanamycin(Kan)选择压力为25mg/L。获得的部分Kan抗性植株经PCR检测,扩增出特异条带,初步证明目的基因已整合到‘北林黄’基因组DNA中。本研究将为进一步利用分子手段对地被菊进行遗传改良,并获得综合抗逆性提高的优异种质奠定基础。     

Substrate integrated waveguide bandpass filter based on butterfly radial slot

A novel broadband substrate integrated waveguide bandpass filter was presented based on the highpass characteristics of substrate integrated waveguide(SIW) and the bandstop performance of butterfly radial slot photonic band gap(PBG) structure. In order to verify this idea,a wideband bandpass filter with 4.65 GHz center frequency and 40% fractional bandwidth was designed. The electromagnetic simulation results show good passband characteristics from 3.71 to 5.6 GHz, while the maximun insertion loss is about 0.40dB. An experimental filter prototype was fabricated with standard PCB process and measured by the vector network analyzer. The measured results show that, for the filter prototype, the passband is from 3.78 GHz to 5.76GHz, the fractional bandwidth is about 41%, the minimal insertion loss is about 0.72 dB and the maximun insertion loss is 1.65.dB The measured results are in good agreement with the simulated results.     

A fuzzy logic multicasting algorithm for ad hoc networks

To increase the multicasting efficiency of Ad hoc networks, a fuzzy logic multicasting algorithm (FLMA) is proposed. FLMA adopts the fuzzy logic to tolerate the imprecise information caused by dynamic network topology. The two input variables of the fuzzy logic system are the relative degree of the additional coverage node number and the relative degree of the residual energy. The deferring time of rebroadcast is the output variable of the fuzzy logic system, which is used to optimize the priority of the nodes to rebroadcast. FLMA reduces the redundant retransmission and the chance of the contention and collision, while balances the energy consumption of the nodes. Simulation results reveal that the FLMA achieves better performance than BCAST in terms of the network lifetime, average end-to-end delay, the average number of drops per node and the throughput.     

Stiffness optimization of vehicle engine magneto-rheological fluids mounts

As important tool for engine semi-automatic isolation system, the magneto-rheological fluids (MRC) can’t achieve tunable stiffness but damping. Therefore, the MRC’s initial stiffness affects the performance of isolation system. To obtain the better isolation effect of MRC in wide frequency, it is necessary to optimize the initial stiffness. As motor unbalanced force and isolation damping are not considered in traditional mount optimization, the feature of semi-automatic control can not be reflected. For a four-cylinder four-cycle in-line diesel engine, a new method which is suitable for stiffness optimization on vehicle engine isolation is presented by using Genetic Algorithm. The optimal isolation system has small absolute force transmissibility in abroad frequency range and small vibration intensity with impact load by using the new method.     

小菜蛾对抑太保的敏感性及其酰胺酶的活力

不同虫龄小菜蛾对抑太保的敏感性差异较大 ,其中 4龄是 1龄的 1 1 .6倍 .不同温度下抑太保对 3龄幼虫的 LC50 随温度的升高而迅速下降 ,1 0℃时为 1 2 43 .94mg·L- 1 ,3 5℃时则下降到 5 .0 6mg·L- 1 .酰胺酶的米氏常数 (Km)为 3 .65× 1 0 - 5nmol,最大反应速度 (Vm ax)为 0 .1 465 nmol· mg- 1 ·min- 1 .4龄幼虫酰胺酶的比活力和总活力随温度的升高而增大 .4℃饲养 1 5 d、1 0℃饲养 1 0 d及 2 5℃饲养条件下 ,4龄幼虫酰胺酶的比活力差异不大 ;但 3 5℃饲养 2 d与前 3个饲养条件相比 ,4龄幼虫酰胺酶的比活力差异较大 .酰胺酶的比活力和总活力均随虫龄的增长而增大 ,4龄与 1 -3龄相比酰胺酶的比活力差异较大 ,不同虫龄酰胺酶的总活力差异显著     

杀球灵抗鸡球虫的药效研究

采有笼饲试验和围栏试验,研究了一种新的抗球虫药——杀球灵抗鸡球虫的药效。笼饲试验表明:杀球灵抗柔嫩艾美耳球虫的指数为195.6,属于高效抗球虫药.围栏试验表明:杀球灵以1ppm剂量添加于饲料,可有效地控制鸡的3种主要球虫——柔嫩、堆型和巨型艾美耳球虫的混合感染,可阻止死亡、抑制盲肠病变产生和卵囊形成,并能维持正常的增重和饲料转化率。