植物与土壤中醇类化合物对高寒草甸退化指示作用初探

醇类化合物是一类重要的生物标记物,被广泛用于土壤及沉积物中有机物质的溯源。为探究醇类化合物对高寒草甸退化指示作用规律,采用野外调查与气相色谱质谱联用(GC-MS)分析方法,对高寒草甸植物与土壤中醇类进行测定和分析。结果表明,高寒草甸植物中醇种类数为2~21,碳数为4~37,不饱和醇种类数高于饱和醇。退化土壤中检出9-甲基-10-顺式十五烯-1-醇、2-亚甲基-5α-胆甾烷-3β-醇、1-三十七烷醇,而在未退化土壤中未检出。高寒草甸中植物和土壤醇的种类大小关系为退化植物退化土壤未退化植物未退化土壤,植物和土壤中相同的醇有很强的相关性(R2=0.871)。植物和土壤醇类的分布特征及相关性分析表明,醇类化合物可以用作标记物来指示高寒草甸退化。     

串叶松香草的引种研究

通过对串叶松香草的形态特征、生物学特性、产量及营养价值等项目的观察和测定,对其利用价值进行了综合评价,指出串叶松香草是一种有前途的优质多年生牧草.     

新形势下基层农业技术推广队伍的调查研究——以河北省为研究案例

中国农技推广队伍曾为农业的发展作出了巨大的贡献,但是随着经济的发展,这一队伍中暴露出了许多与新形势不适应的问题。为了发现并解决这些问题,课题组以河北省为研究案例,对玉田等县的农技推广队伍的现状进行了调研,在此基础上,提出了农技推广队伍发展的相应建议。     

Enterococcus faecium NCIMB 10415 supplementation improves the meat quality and antioxidant capacity of muscle of broilers

The present study was conducted to investigate the effects of Enterococcus faecium (E. faecium) on the meat quality and antioxidant capacity of muscle in broilers. A total of 600 Arbor Acre broiler chickens (1‐day‐old, male) were randomly divided into five treatments with six replicates (20 chickens per replicate) for each treatment. The five treatments were the control treatment (CON, basal diet), antibiotic treatment (ANT, basal diet supplemented with 0.1% chlortetracycline) and E. faecium‐supplemented treatments (LEF, MEF and HEF, basal diet supplemented with 50, 100 and 200 mg/kg of E. faecium respectively). The experiment lasted 42 days in two periods of 21 days. Results showed that there were no differences in breast meat quality among different treatments (p > 0.05). Compared with the CON and ANT treatments, the yellowness of thigh meat in E. faecium‐supplemented treatments was significantly increased (p < 0.05); the shear force of thigh meat in the LEF and MEF treatments was lower than that of the CON treatment (p < 0.05). In addition, the concentration of the inosine monophosphate (IMP) in the breast and thigh meat of the MEF treatment was significantly higher than that of the other treatments (p < 0.05). At 21 days, the total antioxidant capability (T‐AOC) level and glutathione peroxidase (GSH‐Px) activity of breast meat and superoxide dismutase (SOD) in the thigh meat of the MEF treatment were greatly increased (p < 0.05). At 42 days of age, the catalase (CAT), GSH‐Px and T‐AOC activities in the breast meat of the MEF treatment were increased (p < 0.05) and the CAT activity of thigh meat in the LEF and MEF treatments was increased (p < 0.05). In conclusion, E. faecium supplementation increased the meat quality of the thigh muscle, increased the IMP content and the activities of CAT, SOD, T‐AOC, and GSH‐Px of muscle in broilers. Supplementation with 100 mg/kg E. faecium had the greatest effects.     

早生桑品种育151、育237的育成

采用杂交方法育成早生桑品种育151、育237,具有发芽早、产叶量高,叶质较优、抗黄化型萎缩病较强等特性,发芽期比早青桑捉早2-3天.比湖桑32号早8天左右.产叶量提高6-41%.     

凉山皮寒药药效初探

为了探讨凉山皮寒药抑菌、抗炎、调节免疫及抗疲劳的效果,本研究进行了抑菌试验、二甲苯致小鼠耳廓肿胀试验、免疫相关试验及负重游泳试验.结果表明,凉山皮寒药水针剂对选用了6种细菌具有一定的抑菌效果;能使小鼠耳廓肿胀度明显降低,与空白对照组比较,高、中剂量组均差异极显著(P＜0.01),低剂量组差异显著(P＜0.05);对小鼠T淋巴细胞有增值的趋势;能增加脾脏指数;能极显著延长小鼠负重游泳的时间(P＜0.01).结果提示,凉山皮寒药水针剂对部分革兰阳性和阴性菌有一定抑制作用,对小鼠耳廓肿胀有明显的抑制作用,能增强小鼠免疫力与抗疲劳能力.     

猪繁殖与呼吸综合症病毒单克隆抗体的制备和鉴定

以猪繁殖与呼吸综合征病毒（PRRSV）作为免疫原,免疫BALB／c小鼠,经间接ELISA、IPMA和IFA试验进行杂交瘤筛选,共获得2株能分泌针对PRRSV单克隆抗体（简称单抗）的杂交瘤细胞株,分别命名为3D10和4H11,3D10亚类为IgG1,4H11亚类为IgG2b,单抗腹水的间接ELISA效价均达到1．0×10^7,染色体数目介于90～110之间。2株单抗与猪细小病毒、猪伪狂犬病毒、猪圆环病毒2型均无交叉反应,IPMA和IFA结果显示单抗均能与接种于猴肾细胞（Marc145）的PRRSV发生特异性反应,证实抗PRRSV单抗具有良好的特异性和敏感性,为PRRSV抗原表位分析及相关抗原抗体诊断试剂盒的研制奠定了基础。     

甘肃省靖远县生猪定点屠宰场 “瘦肉精”检验报告

为逐步规范靖远地区动物及动物产品市场的质量安全,构建“瘦肉精”等违禁药物长效监管机制,维护公共卫生安全和人民群众身体健康,靖远县动物卫生监督所决定对本地区生猪进行宰前环节“瘦肉精”抽样检测,其目的在于了解靖远地区上市猪肉的健康状况,为下一步动物卫生监督工作有重点高效率的完成做好准备。     

大骨鸡MSTN基因的表达检测

以大骨鸡为试材,采用RT-PCR法检测了MSTN基因在大骨鸡不同器官中的表达情况。结果表明,MSTN基因在大骨鸡骨骼肌中表达水平较高,在心肌、肾脏、脑、肠、舌中也有微量表达,但在肺、肝脏中未检测出MSTN mRNA,而且在14日龄时表达水平高于35和56日龄。