应用TLC-CMS-IR技术检测北芪菇中的L-硒代蛋氨酸

采用薄层层析结合酶解法,从北芪菇中提取、制备硒代蛋氨酸,并对其进行表征分析。结果表明,经酶解展开(正丁醇∶乙酸∶水(7∶1∶2,V/V))和显色后,北芪菇浸提液出现紫色斑点数为4个,其中有1个与硒代蛋氨酸标准品斑点位置一致,其Rf值为0.368;质谱检测显示,北芪菇样液和硒代蛋氨酸标准品基峰质荷比(m/z)均为196 m/z;红外光谱分析显示,北芪菇样液和硒代蛋氨酸标准品均含有N-H,O-H,C-O,C-N,C-Se等功能团;北芪菇中硒代蛋氨酸含量为0.24%。     

外来杂草睫毛婆婆纳的化感作用

睫毛婆婆纳(Veronica hederifolia L.)又名常春藤叶婆婆纳,为玄参科婆婆纳属越年生或一年生草本植物.睫毛婆婆纳原产欧洲、西亚和北非等地中海沿岸国家,是当地极为重要的麦田杂草.该种在20世纪80年代传入我国,在江苏南京和浙江定海有分布.通过其地上部分的水浸提液对小麦、油菜、早熟禾和高羊茅种子的萌发培养试验,研究睫毛婆婆纳的化感效应.结果表明,睫毛婆婆纳地上部分的水浸提液对4种受试植物种子萌发、根长和幼苗干重均有显著的抑制作用,浓度越高,抑制作用越强.在3个生测指标中,根长表现最敏感.在最高浓度(0.20 g/ml)下,高羊茅、早熟禾、小麦和油菜根长的化感效应指数分别为-0.392 9、-0.511 5、-0.118 0和-0.776 5.不同植物对睫毛婆婆纳化感作用的敏感程度也不同, 油菜最敏感, 其种子萌发、根长和幼苗干重在最高浓度(0.20 g/ml)下的化感效应指数分别为-0.345 4、-0.776 5和-0.388 9.     

Effects of oxidative stress and inflammatory response on kidney injury in mice with hyperthyroidism

AIM To explore the effects of oxidative stress and inflammatory response on kidney injury induced by hyperthyroidism in mice. METHODS Forty male Kunming mice were randomly divided into control group （n=20） and L-thyroxine （T4） group （n=20）. The mice in T4 group were intraperitoneally injected with T4 diluent at a dose of 1 mg/kg to induce hyperthyroidism, and those in control group were injected with normal saline of the same volume. After 7 weeks, the mice were weighed and dissected, the kidneys were removed and weighed, and the length of tibia was also measured. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the kidney tissues were detected. The pathological changes of the kidney tissues were observed by HE staining. The levels of 4-hydroxynonenal （4-HNE）-modified proteins, interleukin-1 receptor-associated kinase 1 （IRAK1） and tumor necrosis factor receptor-related factor 6 （TRAF6） were determined by Western blot and immunohistochemistry. RESULTS Compared with control group, the body weight of the mice was decreased, while the kidney size and weight were increased significantly in T4 group. In addition, the ratios of kidney weight/body weight and kidney weight/tibia length were also increased （P<0.05）. In T4 group, the renal tubules were enlarged, and the epithelial cells of renal tubules were swollen and exfoliated, with vacuolar degeneration. Furthermore, reduced SOD activity, and increased MDA content and 4-HNE-modified proteins were found in T4 group, all of which were related to oxidative stress （P<0.05）. The levels of inflammation-related proteins IRAK1 and TRAF6 were significantly increased in T4 group （P<0.05）. CONCLUSION Excessive T4 may lead to kidney hypertrophy and injury in mice, and the mechanism may be related to oxidative stress and inflammatory response.     

^60 Co-γ射线辐射对苋菜种子发芽率及幼苗生长的影响

用不同剂量的^60 Co-γ射线对苋菜种子进行辐射，观测其对苋菜种子发芽率和幼苗生长的影响。结果表明，在该实验设计的辐射剂量范围（20-200Gy）内，对苋菜种子有刺激生长的作用，20Gy有利于苋菜种子发芽，60～80Gy有利于苋菜幼苗的生长。     

不同臭氧浓度对水培烤烟吸收~(32)P的影响

应用3 2 P研究了不同臭氧浓度对水培烤烟吸收磷素营养的影响。结果表明 :在水培法中低浓度臭氧使根对磷素吸收量所占全植株的百分率随培植时间而减少 ,而茎则随培植时间而增加 ;高浓度臭氧则在整个培植期间根、茎的吸收变化不大。两种培植方法磷素在叶中的分布情况都是 :下叶 >中叶 >上叶。烟株各部分的吸收情况 ,根 >茎 >下叶 >中叶 >上叶 ,且两种培植方法对磷的吸收量都与培植时间呈显著正相关 ,高浓度比低浓度臭氧吸收的磷多而快     

庆城县黄花菜生产现状及发展建议

在分析庆城县黄花菜生产现状和存在问题的基础上,从加大宣传力度,提高庆阳黄花菜的知名度;依托企业打造品牌,开展黄花菜绿色认证;加强产中、产后服务,提高黄花菜的经济效益等方面提出了庆城县发展黄花菜生产的建议。     

陕西太平森林公园旅游联动效应分析

以陕西省太平森林公园2005年5月、10月调查资料为基础,从经济联动效应、对社会就业的贡献、产业关联比三方面进行系统分析.结果表明:该公园旅游经济联动效应为1.00∶3.06,直接与间接就业人数比为1.00∶1.71,由旅游业带动的相关行业关联比为1∶6.在分析该公园旅游业目前发展状况的基础上,探讨了其在开发中存在的问题,提出了进一步发展的建议.     

浅析农业科技化后农民的出路

阐述了科学技术发展及其在农业中的运用给农民带带的喜悦及烦恼。针对农业科技化后农民的出路问题提出对策。     

江苏省长江以北地区麦田杂草群落的定量分析

用 7级目测法调查江苏省长江以北地区 6 9个样点的 6 38块麦田共计约 5 4 5hm2 的草害情况 ,数据转化成综合值后 ,进行主成分分析 ,并赋予生态学意义上的解释。研究结果表明 ,决定江苏长江以北地区麦田杂草发生、分布和危害的主要因素是土壤水分、土壤性质 地带性气候因素 ;主成分分析结果表明 ,生境相似 ,杂草群落有趋同性 ;可将 6 9个样点划分为 3个聚类群 ,即沿海旱茬麦类群、内陆旱茬麦类群和稻茬麦类群 ,各聚类群均有相应的优势杂草 ,粘毛卷耳、波斯婆婆纳、刺儿菜、猪殃殃等为沿海旱茬麦田优势杂草 ,内陆旱茬麦田的主要杂草是播娘蒿和麦家公 ,稻茬麦田的杂草优势种为硬草、棒头草 ,并就该地区杂草发生的特点提出了综合治理的建议     

玉米吸收磷素营养的代谢及分布

应用^32P示踪研究玉米的磷素代谢及分布,结果表明：^32P经玉米吸收后,代谢各成分所占百分数：RNA－^32P最多为61．26％,DNA－^32P为23.08%,脂类－^32P为1．32％,有机-^32P为13．04％,无机-^32P最小为1．30％。整株吸收率与时间成显著正相关。说明磷素在玉米植株体内的转化、代谢活动较强。