饲料中的转基因成分在蛋鸡体内代谢残留的研究

用含20%抗草甘膦转基因豆粕的饲料喂养鸡蛋,通过检测其内脏、肌肉、血液、粪便及肠道微生物的豆粕转基因成分,探讨饲料中转基因成分在鸡蛋体内的代谢残留状况。以定性PCR检测含20%转基因豆粕的饲料及蛋鸡的8种内脏样品(肠、胰腺、食道、肝、腺胃、肾、心脏、肌胃)、肌肉、血液、蛋品、粪便、肠道微生物的转基因豆粕的工具基因CaMV35S启动子与NOS终止子以及目的基因CP4-EPSPS等外源基因成分。PCR检测结果表明:除了含20%转基因豆粕的饲料外,蛋鸡的8种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未检出转基因豆粕的CaMV35S、NOS、CP4-EPSPS等外源基因成分。检测结果表明:长期喂养含20%转基因豆粕饲料的蛋鸡的8种内脏器官、肌肉、血液、蛋品、粪便及肠道微生物中均未发现有转基因成分残留,说明饲料中的转基因成分,通过肠胃消化降解后吸收,不会在鸡的组织器官内残留。     

Design, synthesis, crystal structure analysis, and insecticidal evaluation of phenylazoneonicotinoids

On the basis of research of the proposed modes of action between neonicotinoids and insect nicotinic acetylcholine receptor (nAChR), a series of phenylazoneonicotinoids were designed and synthesized to further promote the π-π interaction between molecule and amino acid residues. The target compounds have been identified on the basis of satisfactory analytical and spectral ((1)H NMR, (13)C NMR, HRMS, and X-ray) data. The preliminary results revealed that tiny differences in substitutes resulted in different configurations and great bioactivity variations. Some compounds with electron-donating groups on positions 2 and 6 of the phenyl ring presented higher insecticidal activity than imidacloprid against cowpea aphids ( Aphis craccivora ). The impressive crystal structure of the excellent insecticidal activity compound 9q clearly proved that the functional electronegative pharmacophore was approximately vertical to the methyleneimidazolidine plane. The differences in the mode of interaction on nAChR of typical compounds 9h and 9q remain unclear.     

绿茶-火龙果复合饮料制取工艺研究

为优化绿茶-火龙果饮料的制取工艺,以绿茶、火龙果为主要原料,辅以一定的柠檬酸和蔗糖,配制出风味独特营养丰富的火龙果-绿茶饮料。设计单因素试验以确定正交试验各因素筛选水平范围,结合正交试验分析结果和感官综合评分,探讨火龙果绿茶饮料的生产工艺和操作要点。结果表明：绿茶与火龙果汁复合饮料的料比为2:1,脱脂奶添加量为9%,柠檬酸添加量为0.20%,蔗糖添加量为4%;在此生产工艺条件下,所得产品呈翠绿色、口感佳、稳定性良好,蛋白质含量为9.34 mg/mL,V_c含量为 438 μg/mL,总糖含量为2.5%,茶多酚含量为11.5%,无超标重金属、无菌类污染,可在实际生产中加以应用。     

The feature of clonal expansion of TCR Vβ T cells from peripheral blood in patients with acute monoblastic leukemia

AIM: To investigate the distribution and clonal expansion of TCR Vβ subfamily T cells in patients with acute monoblastic leukemia (M5). METHODS: The CDR3 of TCR Vβ 24 subfamily genes were amplified in peripheral blood mononuclear cells from 9 cases with M5 using RT-PCR and the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluate clonality of the detectable TCR Vβ T cells. RESULTS: Only 1-10 Vβ subfamily T cells were identified in M5 cases. Genescan analysis showed that oligoclonal (clonal expansion) T cells were found in some Vβ subfamilies from 8 cases with M5, Vβ 2 oligoclonal T cells were identified in six cases, whereas Vβ 7- or Vβ 9 clonal expansion T cells were detected in the other two cases, respectively. In addition, except Vβ 2, Vβ 7 or Vβ 21 oligoclonal T cells could be detected in two cases, respectively. CONCLUSION: The skew distribution and clonal expansion of TCR Vβ subfamily T cells could be found in patients with M5.It may be a specific ant i-leukemia immune response with which the host T cells were activated by the leukemia-associated-antigen.The clonal expansion T cells were tendentious in Vβ 2,which may be related to the M5 leukemia cells as sociated antigen.     

纸筒育苗移栽甜菜高产优质栽培试验的模糊分析

本文对纸筒育苗移栽甜菜的４ 因素二次通用旋转试验设计结果进行了模糊处理, 即首先对根产量和含糖率指标进行了模糊量化合成, 再以上述两个指标的合成值与密度（ ｘ１） 、Ｎ（ ｘ２） 、Ｐ２ Ｏ５（ ｘ３） 、Ｋ２ Ｏ（ ｘ４） 施用量建立４ 因素对合成值的回归模型。对模型寻优结果表明,当密度在７９９２０ 株／ｈｍ２ ,施肥量分别为Ｎ １４１．５／ｈｍ２ 、Ｐ２ Ｏ５ １６２．５ｋｇ／ｈｍ２ 、Ｋ２Ｏ ５８ ．４ｋｇ／ｈｍ２ 时可获最高的根产量和含糖;模糊合成值与４ 因素关联度分析结果表明,其顺序为Ｒ１（ 密度） ＞ Ｒ３（Ｐ２Ｏ５） ＞ Ｒ２（ Ｎ） ＞ Ｒ４（Ｋ２ Ｏ） ,说明密度与根产量关系最为密切, 其次为施磷肥量。生产上应首先考虑建立一个合理的群体结构,然后增施磷肥,做到Ｎ、Ｐ、Ｋ 肥合理搭配,才能获得较高的产量与含糖     

飞龙4L─0．75小型联合收割机清选装置的试验研究

将单风道风机加双层振动筛的清选装置改为双风道风机加双层振动筛的形式并进行了台架优化试验，在不附加杂余回收机构的条件下，可保证清选损失率小于０．５％、含杂率小于２％，使小型联合收割机的含杂率指标达到了国家标准的要求     

低碳农业经济与循环农业经济发展简析

从农村的生产方式、农民素质、技术研发和服务体系三方面分析低碳农业经济存在问题,论述传统农业经济发展方式与循环农业经济之间的差距,并提出农业废弃物的再利用与资源化、农业循环型产业链等循环农业方式,促进农业经济健康发展,提升农业经济的整体发展质量。     

常规粳稻超高产栽培的精确定量化——设计与实现

2005年在江苏武进水稻超高产精确定量栽培攻关。试验实施前,针对武进试验点粳稻新品种武运粳7号生育规律、产量形成特性,根据当地的生态条件,运用精确定量栽培的技术原理,以产量800 kg/667 m~2为目标设计栽培方案,包括穗粒结构、播栽期、栽插基本苗数、氮肥用量与运筹比例等关键栽培技术进行了定量化设计,并在试验过程进行了严格的实施。结果表明,试验保证了武运粳7号超高产量潜力的表现,平均产量达803 kg/667 m~2。     

防治稻纵卷叶螟新药剂筛选试验初报

稻纵卷叶螟是对水稻,特别是单季稻生产危害最严重的虫害之一,而药剂施用是目前最主要的防治措施。由于甲胺磷高毒高残留,在生产上已被禁用,因此需选择效果好、毒性低、药本省的药剂替代甲胺磷防治稻纵卷叶螟。试验表明,每667秆用15％氟铃·唑磷乳油60～80ml或50％敌·氟腈乳油90ml,在2、3龄高峰期加水30kg细喷雾,药后10d对稻纵卷叶螟的杀虫效果达95％,保叶效果达80％～85％。     

超高产小麦产量及旗叶生理特性的研究

为给超高产小麦生产能力的进一步提升提供理论依据,以超高产小麦品种温麦6号为对照,在水肥条件较好的高产麦田研究了两个具有超高产潜力的小麦品种洛麦21号和周麦18的产量及生育后期旗叶生理特点。结果表明,与温麦6号相比,洛麦21号和周麦18分别增产13.02%和9.92%,差异达极显著和显著水平。洛麦21号和周麦18每公顷穗数与对照差异不显著,但穗粒数和千粒重均显著高于温麦6号,两品种的经济系数也均高于温麦6号。洛麦21号和周麦18在生育后期都具有较高的光合速率、较大的光合叶面积指数和较高的PSⅡ最大光化学效率(Fv/Fm),并且其光合功能期长,叶绿素含量高;旗叶可溶性糖和可溶性蛋白含量不仅高而且下降缓慢。这些结果说明,改善生育后期旗叶光合性能、延缓叶片衰老进程、增加穗粒数和千粒重,以及提高经济系数可进一步提高超高产小麦品种的产量。