亚热带禾本科牧草闽牧42的植物学特征和利用价值

主要介绍了闽牧42的植物学特征和利用价值。闽牧42是福建省农业科学院甘蔗研究所以丰产、适应性广的甘蔗栽培品种Co419为母本,以大茎野生蔗的杂种后代PT43-52为父本,经有性杂交选育出用作饲草的新型的C     

高等学校林科类专业改造和建设的研究与实践

在对林科类专业改造和建设的必要性与紧迫性分析的基础上,全面介绍了浙江林学院面向农业农村农民("三农"),进行林科类专业改造和建设的成功经验,从而得出4点有益的启示:专业改造和建设的成效直接影响到学校的可持续发展;充分考虑自身条件,立足特色,寻找改革的着力点与突破口;以教学改革为核心,有利于学校统揽全局,推动学校其他工作;专业改造和建设应内外结合,突出重点,重拳出击.参4     

基于演化博弈的农村土地征收补偿分析

随着我国城镇化的深入,城中村及城市边缘农村成为改造、开发的主要对象,农村土地征收带来的政府与农户之间的利益矛盾也越来越突出。基于演化博弈理论,对当地政府和被征收农民的纠纷进行溯因,发现土地征收的主要症结在于当地政府是否愿意放下强势地位,让利于民,由此提出通过将地方政府声誉及公信力收益化,激励当地政府提高征地的货币补偿或提供其他生活保障,以保证农村土地征收的顺利进行。     

柑橘绿霉病拮抗细菌的筛选及其抑制效果

从根际土壤中筛选出1株对柑橘绿霉病菌Penicillium digitatum Sacc.具有较强拮抗活性的菌株HA-01。采用平板对峙法,测定了该菌株对柑橘绿霉病菌、柑橘青霉病菌Penicillium italicum、柑橘酸腐病菌Geotri-chum candidum等12种果蔬病原菌的拮抗活性。结果表明,菌株HA-01对12种供试病原菌均有不同程度的拮抗作用,表现出广谱抗菌活性。菌落形态观察、生理生化特性分析、16S rDNA序列同源性比对以及特异性PCR检测结果表明,该菌株为解淀粉芽孢杆菌Bacillus amyloliquefaciens。对马水橘的活体接种试验表明,菌株HA-01的发酵液、菌悬液和发酵滤液处理3 d后柑橘绿霉病的发病率均低于20%,病斑直径均在2.00 cm以下,而对照发病率为94.44%,病斑直径为3.60 cm。     

南海8种低值小杂鱼脂肪含量和脂肪酸组成分析

采用氯仿-甲醇法提取南海盛产的8种低值小杂鱼肌肉、鱼头、内脏中的粗脂肪,利用气相色谱分析其脂肪酸的组成。结果表明,低值小杂鱼头部粗脂肪含量为2.7%～9.7%,内脏粗脂肪含量为2.9%～6.2%,肌肉部分粗脂肪含量为2.0%～8.5%;不同低值小杂鱼的脂肪酸组成基本相同,但其含量有所差异,饱和脂肪酸含量最高,都在40%以上,内脏粗脂肪中的饱和脂肪酸含量较头部和肌肉中的含量高;多不饱和脂肪酸含量差异较大,粗脂肪含量较高的鱼中多不饱和脂肪酸的含量较低;不同鱼种不同部位的粗脂肪中EPA、DHA含量有所差异。蓝圆鲹的头部粗脂肪中DHA和EPA最高,含量达到28.4%,而双带海鲱鲤内脏粗脂肪中的EPA和DHA含量仅为7.26%。     

不同强度的UV-B辐射对蚕豆种子萌发及幼苗生长的影响

在0～0.3 W/m2的6个UV-B辐射强度处理下,对蚕豆种子萌发及幼苗的初期生长阶段进行研究.结果表明:本试验条件下增强UV-B辐射对蚕豆种子的萌发没有显著影响,对幼苗上胚轴的伸长生长有显著抑制作用;在强度大于0.15 W/m2时,植物可能通过加强呼吸来减轻伤害,使幼苗可溶性糖含量显著减少,从而导致幼苗生物量的降低;不同强度的UV-B辐射降低叶绿素a、b及总叶绿素含量;强度大于0.20 W/m2时,幼苗的MDA含量显著升高,表明膜系统受UV-B辐射的损伤;而本试验条件下增强UV-B辐射对可溶性蛋白含量无显著影响.     

Biological characteristics of bone marrow mesenchymal stem cells from GFP transgenic mice transfected with human β-nerve growth factor gene

AIM:To investigate the changes of biological characteristics of GFP transgenic mouse bone marrow mesenchymal stem cells (MSCs), which were transfected with human β-nerve growth factor (β-NGF) gene in a recombinant eukaryotic expression vector. METHODS:MSCs obtained from GFP transgenic mice were isolated, cultured and purified by the whole bone marrow adherence methods. Human β-NGF gene was transfected into the MSCs by a recombinant eukaryotic expression vector. The β-NGF expression in the MSCs was detected by the method of immunocytochemistry. Hippocampal neurons from neonatal mice were cultured with culture supernatant of the MSCs transfected with pcDNA3-β-NGF and the biological characteristics of the MSCs were investigated 3 d after culture under inverted phase-contrast microscope. RESULTS:The β-NGF positive rate of MSCs in pcDNA3-β-NGF transfection group ［(37.12±2.14)%］ was significantly higher than that in MSCs control group ［(2.36±0.62)%］ and blank control group ［(1.43±0.76)%］.The neurite length of neonatal mouse hippocampal neurons cultured with culture supernatant from pcDNA3-β-NGF-transfected MSCs ［(31±3)μm］ was significantly longer than that in negative control group ［(23±4)μm］, suggesting that MSCs transfected with β-NGF gene maintained better biological characteristics. CONCLUSION: The constructed recombinant eukaryotic expression vector of human β-NGF gene can be transfected into MSCs efficiently and NGF can be effectively expressed in MSCs. MSCs transfected with β-NGF gene are capable of stable expression and secretion of β-NGF, and maintenance of better biological characteristics.     

Mining Applicable Elite Alleles of Growth Duration, Plant Height and Panicle Number per Plant by Conditional QTL Mapping in Japonica Rice

Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between two japonica rice varieties Xiushui 79 and C Bao. The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the e...     

浙江省长柄双花木数量分布与林学特性

长柄双花木为我国特产种 ,产于浙、赣、湘三省 ,属国家二级珍稀濒危保护植物。首次报道了该种在浙江的分布数量 ,共计 3 6 72 0丛 ,数量居三省之首。纠正了前人报道的一些谬误 :①文献认为该种在开化已灭绝 ,经查证分析 ,系前人将分布点龙洞误为龙潭之故 ,本次调查在开化重新找到了该种 ,且发现了大面积分布。②文献记载龙泉分布点海拔为 10 0 0m ,经调查实际为 6 5 0～ 80 0m。③文献记载该种为阴性树种 ,经实地观察 ,认为应属中性树种。调查发现该种在浙江的分布特点与其他两省有所不同 ,一是海拔较低 ,二是生境以沟谷为主。详尽记述了该种在浙江的地理分布、生境特点以及群落学特征 ,分析了群落的演替趋势 ,并就其保护与利用等方面提出了具体建议。参 1 1