Apparent Electrical Conductivity,Soil Properties and Spatial Covariance in the U.S. Southern High Plains

Site-specific soil and crop management will require rapid low-cost sensors that can generate position-referenced data that measure important soil properties that impact crop yields. Apparent electrical conductivity (EC_a) is one such measure. Our main objective was to determine which commonly measured surface soil properties were related to EC_a at six sites in the Texas Southern High Plains, USA. We used the Veris 3100 and Geonics EM-38 EC mapping systems on 12 to 47 ha areas in six center-pivot irrigation sites. Soil samples were taken from 0–150 mm on a 0.1 to 0.8 ha grid and analyzed for routine nutrients and particle size distribution. At four of the six sites, shallow EC_a measured with the Veris 3100 (EC_a-sh) positively correlated to clay content. Clay content was negatively related with EC_a-sh at one site, possibly due to low bulk density of the shallow calcic horizon at that site. Other soil properties that were often correlated with EC_a included soil extractable Ca²⁺, Mg²⁺, Na⁺, CEC, silt and soluble salts. Extractable K⁺, NO₃^–, SO₄^–, Mehlich-3-P, and pH were not related to EC_a. Partial least squares regression (PLS) of seven soil properties explained an average of 61%, 51% and 37% of the variation in observed shallow EC_a-sh, deep EC_a with the Veris 3100 (EC_a-dp) and EC_a with the Geonics EM-38 (EC_a-em), respectively. Including nugget, range and sill parameters from a spherical semivariance model of the residuals from PLS regression improved the fit of mixed models in 15 of 18 cases. Apparent EC, therefore can provide useful information to land-users about key soil properties such as clay content and extractable Ca²⁺, but that spatial covariance in these relationships should not be ignored.     

Runoff Water Quality Impact of Variable Rate Sidedress Nitrogen Application

In recent years, precision agriculture has received attention from producers, agribusiness, and governmental agencies in an effort to increase profitability and protect the environment. Many aspects of precision agriculture, such as soil fertility, application technology, and economic factors, have received substantial research attention; however, other aspects of precision agriculture have not been well documented. One important issue that warrants increased attention is water quality. Because of precision application technology, variable rate fertilizer application based on within-field heterogeneity has the potential to decrease negative water quality impacts. Therefore, the objective of this paired watershed study was to evaluate the impact of variable rate nitrogen (N) fertilizer application on surface water quality. The variable rate field was divided into management units designated as poor, moderate, and high based on measured yield potential and received 100–160 kg/ha of N fertilizer. A portion of the N application was uniformly applied pre-plant or at planting, and rest was sidedressed at variable rates. The uniform rate field received uniform N application at 135 kg/ha. Surface water runoff and water quality were monitored for each field, and collected samples were analyzed for N and phosphorus (P) constituents. During the 2-year monitoring period with 22 storm sampling events, variable rate N application resulted in few water quality differences compared to uniform rate application, but overall median NO₃ + NO₂–N concentrations were significantly lower for the variable rate field in the second year of variable rate N application. Overall and event mean NO₃ + NO₂–N concentrations from the variable rate field tended to be higher, but median concentrations from the uniform rate field tended to be higher.     

Immunohistochemical detection of intermediate filament proteins in formalin fixed normal and neoplastic canine tissues.

Normal and well differentiated neoplastic canine tissues were immunohistochemically stained for keratin, vimentin and desmin intermediate filament proteins using commercially available monoclonal antibodies. Keratin was detected in 56 of 57 carcinomas, vimentin in 59 of 62 sarcomas and desmin in three of four muscle cell tumors. Most normal and neoplastic tissues expressed only one type of intermediate filament; exceptions were one hemangiosarcoma and one pulmonary carcinoma in which there was coexpression of vimentin and keratin proteins. Since immunohistochemical detection of intermediate filaments has tissue-specific distribution in the majority of well differentiated canine neoplasms, these stains may be useful in the differential diagnosis of anaplastic canine tumors. However, the monoclonal antibodies to cytokeratin which were tested in this study failed to detect intermediate filaments in liver, pancreas and salivary glands which suggests that these antibodies may also be unable to detect epithelial tumors derived from these tissues. In addition, in nine neoplasms, the normal tissues adjacent to neoplastic cells failed to stain for the intermediate filament normally expressed. When this occurs, evaluation of intermediate filament expression is invalid for the determination of tissue of origin of the neoplastic cells.     

Congenital Hemolytic Anemia in the Basenji Dog Due to Erythrocyte Pyruvate Kinase Deficiency

Congenital hemolytic anemia in the Basenji dog resembles pyruvate kinase (PK) deficiency in man as it is characterized by an abbreviated erythrocyte life span, an intense reticulocytosis, type II autohemolysis and splenomegaly. Glucose utilization and lactate production were inadequate with respect to the immature cell population. Analysis of enzymes involved in erythrocyte glycolysis revealed a deficiency of pyruvate kinase.     

Hematology of equine fetuses with comparisons to their dams

The Hematologic values of 19 equine fetuses between 202 and 238 days gestation were compared with those of their dams. The red blood cell (RBC) count, hemoglobin concentration, hematocrit, and mean corpuscular hemoglobin concentration were significantly lower in fetal blood, while the mean corpuscular volume, mean corpuscular hemoglobin, and red cell distribution width were significantly higher. Mares had a significantly higher nucleated blood cell count than fetuses, and all nucleated cells were leukocytes (WBC). Most WBC in mare blood were segmented neutrophils and lymphocytes. In contrast, over one-half of the nucleated cells in fetal blood were nucleated RBC, and the majority of WBC in fetal blood were lymphocytes. Mares also had significantly higher plasma protein and fibrinogen concentrations than their fetuses. Mild macrocytosis and mild polychromasia were observed in most fetal blood samples, but not in blood samples from mares. All fetal blood contained reticulocytes, and most samples contained Heinz bodies and Howell-Jolly bodies. The results of this study will contribute to the development of hematologic reference values that may be useful in equine fetal research and, possibly, in the diagnosis of equine fetal disease.     

Clinical and laboratory findings of a bleeding disorder in Simmental cattle

Eight Simmental cattle were presented to the Western College of Veterinary Medicine over the past decade with historic or current prolonged episodes of bleeding. Spontaneous epistaxis, superficial hematomas, and prolonged bleeding following injury or minor surgery (such as tattooing) were frequent observations. Animals presented because of prolonged hemorrhage were anemic and debilitated. Compensatory erythropoiesis was compromised in these animals by depletion of iron stores due to chronic blood loss. Screening tests to assess coagulation were normal as were numbers and morphology of platelets. In vitro platelet aggregation was abnormal in all affected cattle. Three animals were euthanized due to uncontrollable hemorrhage but the precise source of hemorrhage was undetectable. These findings are consistent with bleeding due to a platelet function disorder which has been described previously in Simmental cattle.