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31.
Tritiated digoxin binding to (Na+ + K+)-activated adenosine triphosphatase: possible allosteric site 总被引:9,自引:0,他引:9
Tritiated H(3)-digoxin specifically binds to a cardiac (Na(+) + K(+))-activated adenosine triphosphatase. In the presence of adenosine triphosphate and other nucleoside di- and triphosphates, binding is stimulated by sodium ion, the apparent rate constant being similar to that reported for phosphorus-32 incorporation from adenosine triphosphate and for the adenosine triphosphatase activity. In the presence of magnesium, manganese, inorganic phosphate, or other ions, sodium ion inhibits binding. The data support an allosteric type of sodium-potassium ion pump. 相似文献
32.
33.
A 100-year average recurrence interval for the san andreas fault at wrightwood, california 总被引:1,自引:0,他引:1
Evidence for five large earthquakes during the past five centuries along the San Andreas fault zone 70 kilometers northeast of Los Angeles, California, indicates that the average recurrence interval and the temporal variability are significantly smaller than previously thought. Rapid sedimentation during the past 5000 years in a 150-meter-wide structural depression has produced a greater than 21-meter-thick sequence of debris flow and stream deposits interbedded with more than 50 datable peat layers. Fault scarps, colluvial wedges, fissure infills, upward termination of ruptures, and tilted and folded deposits above listric faults provide evidence for large earthquakes that occurred in A.D. 1857, 1812, and about 1700, 1610, and 1470. 相似文献
34.
Protection against lethal hyperoxia by tracheal insufflation of erythrocytes: role of red cell glutathione 总被引:9,自引:0,他引:9
B S van Asbeck J Hoidal G M Vercellotti B A Schwartz C F Moldow H S Jacob 《Science (New York, N.Y.)》1985,227(4688):756-759
Intact erythrocytes placed into the tracheobronchial tree of hyperoxic rats dramatically improved their chances for survival. Over 70 percent of the animals so treated survived more than 12 days during continuous exposure to 95 percent oxygen, whereas all of the control animals died within 96 hours. Lungs from erythrocyte-protected rats showed almost none of the morphologic damage suffered by untreated animals. Erythrocytes containing cyanomethemoglobin were as beneficial as normal erythrocytes, but cells in which glutathione was partially blocked were significantly less protective. Analogous results were obtained in vitro: 51Cr-labeled target cells released 70 to 90 percent of their label when exposed briefly to hydrogen peroxide or to toxic oxygen species generated by phorbol ester-stimulated neutrophils. Addition of intact erythrocytes decreased release by approximately 75 percent, but significantly less than this if red blood cell glutathione was partially blocked. These results suggest that insufflated erythrocytes, through their recyclable glutathione, protect rats from toxic oxygen species engendered by hyperoxia. 相似文献
35.
36.
Neurohypophyseal hormnones and several synthetic analogs stimulate adenyl cyclase prepared from rabbit kidney medullary tissue. [8-Arginine]-vasopressinoic acid inhibits the stimulation of medullary adenyl cyclase by neurohypophyseal peptides but does not influence the action of parathyroid hormone on adenyl cyclase from kidiney cortex. 相似文献
37.
Molecular biology of learning: modulation of transmitter release 总被引:82,自引:0,他引:82
Until recently, it has been impossible to approach learning with the techniques of cell biology. During the past several years, elementary forms of learning have been analyzed in higher invertebrates. Their nervous systems allow the experimental study of behavioral, neurophysiological, morphological, biochemical, and genetic components of the functional (plastic) changes underlying learning. In this review, we focus primarily on short-term sensitization of the gill and siphon reflex in the marine mollusk, Aplysia californica. Analyses of this form of learning provide direct evidence that protein phosphorylation dependent on cyclic adenosine monophosphate can modulate synaptic action. These studies also suggest how the molecular mechanisms for this short-term form of synaptic plasticity can be extended to explain both long-term memory and classical conditioning. 相似文献
38.
Malignant lymphomas following allogenic disease: transition from an immunological to a neoplastic disorder 总被引:17,自引:0,他引:17
The graft versus host reaction which occurs in F(1) hybrid mice injected with parental spleen cells was used to examine several immunological theories of neoplasia. Long-term survivors of this reaction developed lymphoid neoplasms which resembled Hodgkin's disease and lymphosarcoma. Mice with these tumors were chimeras, but the parental cells present within their spleens had specific immunological tolerance toward host antigens. This, together with the finding that the tumors were transplantable only to isogenic recipients, indicates that the tumors were of host rather than donor origin. 相似文献
39.
Renal concentrating mechanism: possible role for sodium-potassium activated adenosine triphosphatase 总被引:2,自引:0,他引:2
M Martinez-Maldonado J C Allen G Eknoyan W Suki A Schwartz 《Science (New York, N.Y.)》1969,165(895):807-808
Sodium-potassium activated adenosine triphosphatase activity was found to be almost twice as high in renal medulla as in cortex. Infusion of digoxin, a specific inhibitor of the enzyme, into one renal artery of the dog resulted in unilateral natriuresis, impaired concentrating capacity, and reduction of the enzyme activity in both cortex and medulla. It is suggested that the sodium-potassium adenosine triphosphatase plays an important role in urine concentration mechanisms. 相似文献
40.
L S Freedman S Samuels I Fish S A Schwartz B Lange M Katz L Morgano 《Science (New York, N.Y.)》1980,207(4433):902-904
Rates of tyrosine and lysine transport and incorporation into protein were measured in control and undernourished weanling rats. Undernutrition was induced by feeding lactating dams a low protein diet (12 percent casein) from birth to day 21. At weaning, body and brain weights of undernourished rats were 50 percent and 88 percent, respectively, of control values. Lysine and tyrosine transport rates into skeletal muscle were reduced by over 75 percent, more than twice the reduction seen in brain. Rates of amino acid incorporation into muscle protein were reduced by approximately 50 percent; the change in rate of incorporation into brain protein was not statistically significant. These data indicate that, in spite of marked retardation of amino acid transport into brain, the brain seems fully capable of maintaining normal rates of protein synthesis. 相似文献