Consequences of sodium exclusion for the osmotic potential in the rhizosphere – Comparison of two maize cultivars differing in Na+ uptake

According to the biphasic model of growth response to salinity, growth is first reduced by a decrease in the soil osmotic potential (Ψ_o), i.e., growth reduction is an effect of salt outside rather than inside the plant, and genotypes differing in salt resistance respond identically in this first phase. However, if genotypes differ in Na⁺ uptake as it has been described for the two maize cultivars Pioneer 3906 and Across 8023, this should result in differences in Na⁺ concentrations in the rhizosphere soil solution and thus in the concentration of salt outside the plant. It was the aim of the present investigation to test this hypothesis and to investigate the effect of such potential differences in soil Ψ_o caused by Na⁺ exclusion on plant water relations. Sodium exclusion at the root surface of intact plants growing in soil was investigated by sampling soil solution from the rhizosphere of two maize cultivars (Across 8023, Pioneer 3906). Plants were grown in a model system, consisting of a root compartment separated from the bulk soil compartment by a nylon net (30 μm mesh size), which enabled independent measurements of the change of soil solution composition and soil water content with increasing distance from the root surface (nylon net). Across 8023 accumulated higher amounts of sodium in the shoot compared to the excluder (Pioneer 3906). The lower Na⁺ uptake in the excluder was partly compensated by higher K⁺ uptake. Pioneer 3906 not only excluded sodium from the shoot but also restricted sodium uptake more efficiently from roots relative to Across 8023. This was reflected by higher Na⁺ concentrations in the rhizosphere soil solution of the excluder 34 days after planting (DAP). The difference in Na⁺ concentration in rhizosphere soil solution between cultivars was neither due to differences in transpiration and thus in mass flow, nor due to differences in actual soil water content. As the lower Na⁺ uptake of the excluder (Pioneer 3906) was only partly compensated by increased uptake of K⁺, soil Ψ_o in the rhizosphere of the excluder was more negative compared to Across 8023. However, no significant negative effect of decreased soil Ψ_o on plant water relations (transpiration rate, leaf Ψ_o, leaf water potential, leaf area) could be detected. This may be explained by the fact that significant differences in soil Ψ_o between the two cultivars occurred only towards the end of the experiment (27 DAP, 34 DAP).     

Genetic diversity and cytogenetic analyses in <Emphasis Type="Italic">Curcuma zedoaria</Emphasis> (Christm.) Roscoe from Bangladesh

Curcuma zedoaria populations comprise 2n = 63 chromosomes with three satellite chromosomes showing elongated secondary constrictions. Flow cytometry results inferred that the 2C nuclear DNA values varied between the populations. The largest genome size was found in the population Chittagong (mean 3.37 pg) and the lowest in the Birganj population (mean 3.15 pg). RAPD based estimations of genetic diversity revealed that hilly populations maintain higher genetic diversity, which was also found to be distinct from plainland and plateauland populations. Genome sizes and genetic diversity values of the populations were positively correlated.     

Novel lysimeter techniques — a basis for the improved investigation of water,gas, and solute transport in soils

For many years lysimeters have been proven to be effective tools in assessing and predicting the effects of current land use and future land use changes in catchment areas on both water and solute balances. Although due to the diverse aspects of mass transport modelling, many different types of lysimeters exist, water and solute balances are by no means fully understood, especially in post‐mining areas. To tackle this problem, a new piece of equipment has been developed which enables the actual weight of a lysimeter to be precisely measured. The newly designed device, which has been used for one of the experiments described in this paper, permits the weighing of for example a 2 m³ lysimeter vessel with an accuracy down to 30 g. The second newly developed appliance presented here is the GAMS (Gas‐Migration‐Simulator). Basically comparable to a lysimeter, the difference is that the GAMS allows the detailed investigation of soil‐gas migration processes and their dependence on parameters like the diffusion coefficient and the gas permeability of the soil, alterations of the groundwater level and on various external influences such as changes of the actual meteorological conditions. These two newly developed techniques are described in this paper, and their respective suitability is demonstrated on the basis of data sets recorded during initial experiments.     

Spectral reflectance characteristics of citrus canker and other peel conditions of grapefruit

The presence of one fruit infected with citrus canker in a shipment may render the whole shipment unmarketable. Therefore it is important to classify fruit infected with citrus canker in the packinghouse before shipping the produce. The purpose of this research was to determine the significant wavelengths that could be used to classify canker among other peel conditions using grapefruit, a variety susceptible to canker. A spectrophotometer, with a wavelength range of 200–2500 nm, was used to measure the spectral reflectance data of peels from market quality fruit and fruits that were infected with cake melanose, wind scar, copper burn, greasy spot and canker. Statistical data analysis was performed on the spectral reflectance data to identify the wavelengths that had maximum discriminatory potential among the different peel conditions and to derive a discriminant function from the identified wavelengths. The discriminatory wavelengths were identified in the visible and the visible near infrared range. In addition, the classification based on the derived discriminant function resulted in 100% classification of canker. These results provide fundamental and practical use in the development of an automatic fruit sorter for canker classification based on spectral reflectance. Future research would involve the development of a vision-based classification system using the significant wavelengths identified in this study.     

Susceptibility to flavescence dorée of different Vitis vinifera genotypes from north-western Italy

The aim of this work was to evaluate the susceptibility to flavescence dorée (FD) of 12 Vitis vinifera cultivars grown in Piedmont, and representative of the wine-making tradition of this area. The experiments were conducted under controlled conditions to ensure constant infection pressure. Test plants were ex vitro potted vines, singly inoculated with four Scaphoideus titanus infected by FD-C phytoplasma (FDp), under greenhouse conditions. Vines were tested for FDp at 5 and 8 weeks postinoculation (wpi) and the phytoplasma load was measured in leaves and roots at 8 wpi. Within the 14 V. vinifera accessions (belonging to 12 cultivars), three susceptibility clusters were identified. Cultivars within the low susceptibility group showed low phytoplasma loads and low percentages of infected plants, suggesting a tolerant behaviour. To confirm these results, four Vitis cultivars, representing extremes of FD susceptibility from low to high, were grafted onto Kober 5BB rootstocks and inoculated with laboratory-infected S. titanus, under semi-field conditions. The transmission experiments onto grafted cuttings confirmed that susceptibility to the disease depends on the scion genotype. The data indicated that none of the tested V. vinifera genotypes are resistant to FD, although some cultivars with low susceptibility are available, and can be explored for identifying genetic traits involved in disease tolerance/resistance. Moreover, ranking Vitis genotypes for their susceptibility to FD is in itself a valuable tool to support vine growers in their decision management, by helping them to choose the most appropriate varieties according to their specific FD epidemiological contexts.