Saturnine gout: lead-induced formation of guanine crystals

Intravenous injection of a sublethal dose of lead acetate into a domestic pig resulted in a 4.5-fold increase of guanine in the urine, indicating an impairment in the conversion of guanine to xanthine. This impairment is probably due to the inhibition of guanine aminohydrolase (guanase), since the activity of this enzyme is inhibited by Pb2+ (the inhibition constant being 3.0 X 10(-6)M). Postmortem histological examination revealed concretions of crystalline material in the epiphyseal plate of the femoral head. Extraction of the section containing the concretions showed that they were guanine. The relation of these findings to saturnine gout is discussed.     

Trans-suppression of misfolding in an amyloid disease

The transthyretin (TTR) amyloid diseases, representative of numerous misfolding disorders, are of considerable interest because there are mutations that cause or suppress disease. The Val30 --> Met30 (V30M) TTR mutation is the most prevalent cause of familial amyloid polyneuropathy in heterozygotes, whereas a Thr119 --> Met119 (T119M) mutation on the second TTR allele protects V30M carriers from disease. Here, we show that the incorporation of one or more T119M TTR subunits into a predominantly V30M tetramer strongly stabilized the mixed tetramer against dissociation. Dissociation is required for amyloid formation, so these findings provide a molecular explanation for intragenic trans-suppression of amyloidosis. The data also suggest a potential therapeutic strategy, provide insight into tissue-specific deposition and amyloid composition, and support the validity of the amyloid hypothesis in human disease.     

What the Longest Exposures from the Hubble Space Telescope Will eveal

Detailed simulations are presented of the longest exposures on representative fields that will be obtained with the Hubble Space Telescope, as well as predictions for the numbers and types of objects that will be recorded with exposures of different durations. The Hubble Space Telescope will reveal the shapes, sizes, and content of faint, distant galaxies and could discover a new population of Galactic stars.     

Eclipse Measurements of Io's Sodium Atmosphere

The satellites of Jupiter eclipsed each other in 1985, and these events allowed an unusual measurement of the sodium in Io's extended atmosphere. Europa was used as a mirror to look back through the Io atmosphere at the sun. The measured column abundances suggest that the atmosphere is collisionally thin above 700 kilometers and may be collisionally thin to the surface. The sodium radial profile above 700 kilometers resembles a 1500 kelvin exosphere with a surface density near 2 x 10(4) sodium atoms per cubic centimeter, but a complete explanation of the dynamics requires a more complex nonthermal model: the calculated loss rates suggest that the atmosphere is being replaced on a time scale of hours.     

Block Estimating of Spatial Yield Data and its Uncertainty

On-the-go yield monitors have been available for both grain and bulk crops. Most of the yield monitors today provide yield measurement at a fixed time interval. Conversion of these point yield data into raster yield maps for further analysis is necessary. In this study, a data-blocking procedure is proposed to create raster yield maps from point yield data. The blocking procedure includes: (1) converting the fixed-time-interval data into fixed-distance-interval data; (2) using a moving average algorithm to estimate a cell value when there are sufficient data points within the cell; (3) using a geostatistical algorithm to estimate a cell value when there are not enough data points within the cell but values of its neighboring cells are known; and (4) calculating an uncertainty index for each cell value estimation. An example application of the yield-blocking procedure with potato harvest data in 1996 was given.     

Efficacy of murine malaria sporozoite vaccines: implications for human vaccine development

As part of a study of potential vaccines against malaria, the protective efficacy of sporozoite subunit vaccines was determined by using the Plasmodium berghei murine malaria model. Mice were immunized with recombinant DNA-produced or synthetic peptide-carrier subunit vaccines derived from the repetitive epitopes of the Plasmodium berghei circumsporozoite gene, or with radiation-attenuated sporozoites. Immunization with subunit vaccines elicited humoral responses that were equivalent to or greater than those elicited by irradiated sporozoites, yet the protection against sporozoite challenge induced by either of the subunit vaccines was far less than that achieved by immunization with attenuated sporozoites. Passive and adoptive transfer studies demonstrated that subunit vaccines elicited predominantly antibody-mediated protection that was easily overcome whereas irradiated sporozoites induced potent cell-mediated immunity that protected against high challenge doses of sporozoites. These studies indicate that new strategies designed to induce cellular immunity will be required for efficacious sporozoite vaccines.     

Photon emission at molecular resolution induced by a scanning tunneling microscope

The tip-surface region of a scanning tunneling microscope (STM) emits light when the energy of the tunneling electrons is sufficient to excite luminescent processes. These processes provide access to dynamic aspects of the local electronic structure that are not directly amenable to conventional STM experiments. From monolayer films of carbon-60 fullerenes on gold(110) surfaces, intense emission is observed when the STM tip is placed above an individual molecule. The diameter of this emission spot associated with carbon-60 is approximately 4 angstroms. These results demonstrate the highest spatial resolution of light emission to date with a scanning probe technique.